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Kit for measuring salivary liquefied carbohydrate chain antigen KL-6 and preparation method thereof

A sugar chain antigen, KL-6 technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of not paying attention to the stability of latex particles, and achieve the effect of improving anti-interference ability, sensitivity and detection efficiency

Pending Publication Date: 2022-05-27
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This application has the characteristics of high sensitivity and wide linear range, which can realize the accurate detection of sialoligated sugar chain antigens, but the kit does not pay attention to the stability of latex particles in reagent R2. In the future, the sensitivity of the kit after long-term storage

Method used

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  • Kit for measuring salivary liquefied carbohydrate chain antigen KL-6 and preparation method thereof
  • Kit for measuring salivary liquefied carbohydrate chain antigen KL-6 and preparation method thereof
  • Kit for measuring salivary liquefied carbohydrate chain antigen KL-6 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 A kind of salivary liquefied sugar chain antigen assay kit

[0063] The test kit includes reagent R1 and reagent R2;

[0064] Described reagent R1 comprises: Tris buffer solution 80g / L; Sodium chloride 80g / L; Cocamidopropyl betaine 10g / L; Proclin 300 1g / L;

[0065] The reagent R2 includes: tris buffer 80g / L; Tween-80 20g / L; sucrose 5g / L; sialic liquefied sugar chain antigen KL-6 antibody latex particles 20mg / L; Proclin 300 1g / L.

Embodiment 2

[0066] Embodiment 2 A kind of salivary liquefied sugar chain antigen assay kit

[0067] The test kit includes reagent R1 and reagent R2;

[0068] Described reagent R1 comprises: Tris buffer solution 120g / L; Sodium chloride 120g / L; Sodium cocoyl glutamate 30g / L; Proclin 300 1g / L;

[0069] The reagent R2 includes: tris buffer 80g / L; polyethylene glycol 4000 30g / L; sucrose 20g / L; sialic liquefied sugar chain antigen KL-6 antibody latex particles 20mg / L; 1g / L.

Embodiment 3

[0070] Embodiment 3 A kind of salivary liquefied sugar chain antigen assay kit

[0071] The test kit includes reagent R1 and reagent R2;

[0072] The reagent R1 includes: tris buffer 90g / L; sodium chloride 110g / L; sodium lauroyl glutamate 15g / L; sodium azide 1.2g / L;

[0073] The reagent R2 includes: tris buffer 90-110g / L; polyethylene glycol 10000 30g / L; bovine serum albumin 10g / L; sialic liquefied sugar chain antigen KL-6 antibody latex particles 25mg / L; sodium azide 1.2g / L.

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Abstract

The invention discloses a salivary liquefied carbohydrate chain antigen determination kit, and relates to the technical field of detection kits. The kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a buffer solution, a penetrating fluid, a surfactant A and a preservative; the reagent R2 comprises a buffer solution, a surfactant B, a stabilizer, salivary liquefied sugar chain antigen KL-6 antibody latex particles and a preservative. In the implementation process of the salivary liquefied carbohydrate chain antigen determination kit provided by the invention, a surfactant B in the reagent R2 is controlled to be a mixture of Tween 80 and polyethylene glycol 8000, and a stabilizer is a mixture of cane sugar, spirulina polysaccharide and phycocyanin, so that the stability of latex can be better ensured, the spontaneous agglutination phenomenon cannot occur after long-time placement, and the stability of the kit is improved. Therefore, the detection stability and accuracy are improved.

Description

technical field [0001] The invention relates to the technical field of detection kits, in particular to a salivary liquefied sugar chain antigen KL-6 detection kit and a preparation method thereof. Background technique [0002] The salivary liquefied sugar chain antigen KL-6 is a transmembrane high molecular weight mucin, belonging to the mucin MUC1 family, containing the salivary liquefied sugar chain structure. This sugar chain structure has a spatial structural epitope recognized by a specific antibody. KL-6 is mainly expressed in type II alveolar cells and bronchial epithelial cells. When lung inflammation occurs, massive regeneration of type II alveoli and disruption of the pulmonary interstitial epithelial cell barrier results in a massive release of KL-6 into the blood. Studies have shown that the detection of KL-6 content in blood samples can be used to diagnose lung inflammation, especially the occurrence of interstitial pneumonia. [0003] Interstitial pneumonia...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 章晓庆王志
Owner 浙江夸克生物科技有限公司
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