Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polymer, chip and preparation method and application thereof

A polymer, selected technology, applied in the direction of biochemical equipment and methods, chemical instruments and methods, laboratory containers, etc., can solve the problems of chip manufacturing process and application limitations, low probe density, etc., to overcome Air sensitivity and good stability

Active Publication Date: 2022-06-03
GENEMIND BIOSCIENCES CO LTD
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are still many defects or limitations in the existing chips, which limit their application.
For example, the density of probes that can be carried by the chip substrate is low, which makes the manufacturing process and application of the chip relatively limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polymer, chip and preparation method and application thereof
  • Polymer, chip and preparation method and application thereof
  • Polymer, chip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0113] Embodiments of the present invention also provide a method for preparing the polymer as described above, comprising the steps of:

[0114] The polymer is prepared by copolymerizing monomer A and monomer B; the monomer A forms the repeating unit A, and the monomer B forms the repeating unit B.

[0115] In one specific embodiment, the molar ratio of the monomer A and the monomer B is 1:(1-30). Specifically, the molar ratio of the monomer A and the monomer B is 1:1, 1:3, 1:5, 1:8, 1:10, 1:12, 1:15, 1:20, 1:1 25, 1:30.

[0116] In one specific embodiment, the copolymerization reaction refers to the polymerization reaction of the monomer A and the monomer B under the initiation of an initiator at 30-60° C. (reaction temperature). Specifically, the reaction temperature can adopt the following specific temperature values: 30°C, 35°C, 36°C, 37°C, 38°C, 40°C, 41°C, 42°C, 43°C, 45°C, 50°C, 52°C , 54℃, 55℃, 56℃, 58℃, 60℃.

[0117] In one specific embodiment, the initiator is s...

Embodiment 1

[0137] This embodiment provides a chip, the preparation method of which is as follows (for the process flow diagram, see figure 1 ):

[0138] (1) Polymer preparation

[0139] Using toluene as a solvent, according to the volume ratio, 5wt% of GMA (glycidyl methacrylate) and 0.1wt% of PEGDA (polyethylene glycol (glycol) diacrylate) with a molecular weight of 1000 g / mol were added to the system, 0.1 wt% AIBN (azodiisobutyronitrile; azobisisobutyronitrile, Chinese name is azobisisobutyronitrile) initiator, after nitrogen and oxygen removal, the polymerization reaction occurs at 55°C. After the reaction was carried out for 3 hours, oxygen was injected to terminate the reaction. After methanol was used to extract the polymer, the epoxy-based macromolecules of the body shape were obtained by vacuum drying, and GPC detection (gel permeation chromatography) was used. The detection results are as follows figure 2 As shown, an epoxy-based macromolecule with a peak molecular weight of ...

Embodiment 2

[0151] The present embodiment provides a chip, and its preparation method is as follows:

[0152] (1) Polymer preparation

[0153] Use water and DMF (dimethylformamide) as solvents, wherein the volume ratio of water and DMF is not 4:1. According to the mass ratio, 2% of acrylamide AM, 0.2% of GMA monomer, 0.1% of KPS (potassium persulfate) initiator and 0.01% of TEMED (tetramethylethylenediamine) gel accelerator were added to the system, After nitrogen and oxygen removal, the polymerization reaction occurred at 42°C. After the reaction was carried out for 3 hours, oxygen was injected to terminate the reaction, and methanol was used to extract the polymer, followed by vacuum drying to obtain linear epoxy-based macromolecules, which were detected by GPC (gel permeation chromatography), and the detection results were as follows: Figure 5 As shown, an epoxy-based macromolecule with a peak molecular weight of 191,619 was obtained.

[0154]

[0155] (2) Substrate surface modi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a polymer, a chip and a preparation method and application thereof. The polymer comprises a repeating unit A and a repeating unit B with specific structural characteristics. The biochip can load biological components with higher density, meets the requirements of continuously developing biomolecule preparation and / or analysis, and is good in stability.

Description

technical field [0001] The present invention relates to the technical field of chips, in particular to polymers, chips and preparation methods and applications thereof. Background technique [0002] Biochips are usually made using surface modification techniques and using polymer-coated substrates. Surface modification technology, from simple two-dimensional modification, to 2.5-dimensional modification, to three-dimensional modification, has greatly improved the density of surface functional groups. After the density of functional groups is increased, it can be used for high-throughput testing on the surface of biochips, such as gene chip surface PCR, surface sequencing, etc. [0003] However, the existing chips still have many defects or limitations, which limit their application. For example, the density of probes that can be carried by the chip substrate is relatively low, which limits the fabrication process and application of the chip. SUMMARY OF THE INVENTION [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08F283/06C08F220/32C08F220/56C08F220/60B01L3/00C12Q1/6837
CPCC08F283/065C08F220/56B01L3/502707C12Q1/6837B01L2300/12C08F220/325C08F220/60C12Q2563/107C12Q2565/501C12Q2565/629B01J20/267B01J20/3234B01J20/3278B01J20/3219C03C17/3405C09D133/24C09D133/068C08F222/1063B01L2200/12C03C17/30C03C17/32
Inventor 林志峰孙雷王琦冯叠文
Owner GENEMIND BIOSCIENCES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com