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Biological agent for preventing and treating tobacco bacterial wilt as well as preparation method and application of biological agent

A technology for tobacco bacterial wilt disease and biological preparations, which is applied in the field of biological preparations for preventing and controlling tobacco bacterial wilt disease, can solve problems such as pesticide residue environment and pollution, and achieve the effects of reducing environmental pollution, promoting plant growth and improving crop safety.

Pending Publication Date: 2022-06-28
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a biological preparation, preparation method and application for preventing and treating tobacco bacterial wilt, which solves the technical problems of using chemical pesticides to prevent and control tobacco bacterial wilt, pesticide residues and environmental pollution in the prior art

Method used

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  • Biological agent for preventing and treating tobacco bacterial wilt as well as preparation method and application of biological agent
  • Biological agent for preventing and treating tobacco bacterial wilt as well as preparation method and application of biological agent
  • Biological agent for preventing and treating tobacco bacterial wilt as well as preparation method and application of biological agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This example illustrates the acquisition and identification of the Bacillus subtilis YWS-20-2072 of the present invention.

[0033] 1. The method for obtaining Bacillus subtilis YWS-20-2072 of the present invention

[0034] 1.1. Materials

[0035] Perennial tobacco planting area, healthy tobacco plant rhizosphere soil.

[0036] The LB solid medium and the NA medium were thawed, poured into sterile petri dishes, cooled, and made into plates. LB solid medium is used to isolate and preserve endophytic bacteria, and NA medium is used to cultivate tobacco bacterial wilt pathogenic bacteria and antagonistic bacteria to detect the activity of the bacteria.

[0037] 1.2. Separation and culture

[0038] The bacteria in the soil were separated by serial gradient dilution method and plate coating method, and the colonies with different shapes were randomly selected for purification, culture and preservation.

[0039] 1.3. Screening of antagonistic endophytic bacteria

[0040]...

Embodiment 2

[0046] This example describes the biological preparation for preventing and controlling tobacco bacterial wilt according to the present invention.

[0047] The preparation method of the biological preparation for the prevention and treatment of tobacco bacterial wilt in the present embodiment, comprises the following steps:

[0048] S1: Use Bacillus subtilis YWS-20-2072 as the strain, and activate the strain. Specifically, Bacillus subtilis YWS-20-2072 stored at -80°C was inoculated on a liquid LB medium, and cultured at 36°C for 12 h to obtain activated Bacillus subtilis YWS-20-2072.

[0049] S2: culturing the strain of Bacillus subtilis YWS-20-2072 to obtain the first-class seed liquid of Bacillus subtilis YWS-20-2072. Specifically, according to 10% of the inoculum, the activated Bacillus subtilis YWS-20-2072 was inoculated into the primary seed medium, and the culture temperature was 36 °C and the rotation speed was 160 rpm / min, and the shaking table was cultured for 24 ho...

Embodiment 3

[0057] This example illustrates the control effect of the biological preparation for preventing and controlling tobacco bacterial wilt prepared by the method of Example 2 on tobacco bacterial wilt.

[0058] The biological preparation for controlling tobacco bacterial wilt prepared by the method of Example 2 is hereinafter referred to as the Bacillus subtilis YWS-20-2072 preparation.

[0059] 1. The control effect of Bacillus subtilis YWS-20-2072 preparation on tobacco bacterial wilt

[0060] 1.1. Materials and methods

[0061] Indoor antagonistic activity detection: using the Oxford cup method: take 3 mL of the bacterial suspension of tobacco bacterial wilt pathogenic bacteria (1 × 10 6 cfu / mL), fully mixed with 60 mL of melted NA medium cooled to 45°C to prepare a medium containing the pathogenic bacteria of tobacco bacterial wilt; Oxford cups (hole diameter of 6 mm) were placed at 2.5 cm from the center of the edge of the plate. , draw 0.1 ml of the active substance to be ...

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Abstract

The invention discloses a biological agent for preventing and treating tobacco bacterial wilt as well as a preparation method and application thereof, relates to the technical field of tobacco bacterial wilt prevention and treatment, and solves the technical problems of pesticide residues and environmental pollution caused by adopting chemical pesticides to prevent and control tobacco bacterial wilt in the prior art. The preparation method comprises the following steps: by taking bacillus subtilis YWS-20-2072 as a strain, activating the strain; the method comprises the following steps: culturing a bacillus subtilis YWS-20-2072 strain, so as to obtain a bacillus subtilis YWS-20-2072 primary seed solution; the bacillus subtilis YWS-20-2072 primary seed solution is cultured, and a bacillus subtilis YWS-20-2072 secondary seed solution is obtained; the secondary seed liquid of the bacillus subtilis YWS-20-2072 is cultured, fermentation liquid of the bacillus subtilis YWS-20-2072 is obtained, and the fermentation liquid of the bacillus subtilis YWS-20-2072 is the biological preparation for preventing and treating the tobacco bacterial wilt. The biological agent for preventing and controlling tobacco bacterial wilt can effectively prevent and control tobacco soil-borne disease bacterial wilt, and can promote plant growth.

Description

technical field [0001] The invention relates to the technical field of tobacco bacterial wilt prevention and control, in particular to a biological preparation for preventing and controlling tobacco bacterial wilt, a preparation method and application of the preparation. Background technique [0002] Tobacco (scientific name: Nicotiana tabacum) belongs to Solanaceae, an annual herb. In tobacco planting, the occurrence of bacterial wilt is relatively serious, affecting the entire growth period of tobacco. Tobacco bacterial wilt is a vascular bacterial soil-borne disease caused by Ralstonia solanacearum, which has caused huge economic losses to the tobacco planting industry. At present, there is no specific medicine for the control of tobacco bacterial wilt. [0003] A large number of studies have shown that the prevention and control of tobacco bacterial wilt should first select and plant disease-resistant varieties; when transplanting, use 350-400 g / mu of 55% dixon wettabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00C12R1/125
CPCC12N1/20A01N63/22
Inventor 杨润霞赵崇钧毛明林李诚郭绍青潘菲字文霖
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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