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Preparation method of anti-Newcastle disease transfer factor

A technology of transfer factor and chicken Newcastle disease, which is applied in the field of preparation of anti-chicken Newcastle disease transfer factor, can solve the problems of unstable treatment effect and no specificity of disease, and achieves the effect of improving immunity, strong specificity and good effect.

Pending Publication Date: 2022-07-01
DINGZHENG XINXING BIOTECH TIANJIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of preparation in the existing transfer factor technology, lack of specificity for diseases, unstable therapeutic effect, etc., and provide an in vitro immune method with strong specificity, low cost, high output rate and better effect. Preparation method of anti-Newcastle disease specific transfer factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method for preparing anti-chicken Newcastle disease specific transfer factor in vitro:

[0019] (1) Take chicken spleen or chicken blood under sterile environment, and prepare lymphocyte monolayer with chicken spleen or peripheral blood. Take 10ml of animal whole blood from sterile vein, add 0.2ml of 0.8% heparin solution for anticoagulation, let it stand for 20min, and use a pipette with a rubber bulb to absorb all the plasma above the erythrocyte layer, especially the leukocyte layer immediately above the erythrocyte layer. Dispense into sterilized centrifuge tubes, wash 3 times with PBS (pH is 7.2), and centrifuge at 800 rpm, then use 40ml of hydrolyzed milk protein containing 30% calf serum for leukocyte culture, mix well, and then divide into volumes In a 50ml cell culture flask, close the stopper, and place it in a cell culture incubator at 37°C. After 3 days, the leukocytes evenly adhere to the wall, which is a lymphocyte monolayer;

[0020] (2) Su...

Embodiment 2

[0023] A preparation method for preparing anti-chicken Newcastle disease specific transfer factor in vitro:

[0024] (1) Take chicken spleen or chicken blood under sterile environment, and prepare lymphocyte monolayer with chicken spleen or peripheral blood. Take 20ml of animal whole blood from sterile vein, add 0.2ml of 0.8% heparin solution for anticoagulation, let it stand for 40min, and use a pipette with a rubber bulb to absorb all the plasma above the red blood cell layer, especially the white blood cell layer immediately above the red blood cell layer. Dispense into sterilized centrifuge tubes, wash 2-3 times with PBS (pH is 7.2), and centrifuge at 800 rpm, then use 40 ml of hydrolyzed milk protein containing 30% calf serum for leukocyte culture, mix well, and then dispense In a cell culture bottle with a capacity of 100ml, close the bottle stopper, and place it in a cell culture incubator at 37°C. After 2 days, the leukocytes evenly adhere to the wall, that is, a lymph...

Embodiment 3

[0028] The detection of the anti-chicken Newcastle disease specific transfer factor prepared by the process of the present invention The following detection is carried out to the anti-chicken Newcastle disease transfer factor (hereinafter referred to as "this product") prepared by the process described in Example 1:

[0029] (1) The standard solution is a yellow clear liquid with a pH value between 6.1-7.0;

[0030] (2) Determination of polypeptide content: the polypeptide content of this product is 0.47mg / ml determined by the double-shrinkage method;

[0031] (3) Determination of nucleic acid content: The nucleic acid content of this product was determined by the lichenol method to be 0.39 mg / ml;

[0032] (4) Bacteriological testing: the presence of oxygen, anaerobic, saprophytic bacteria and fungi is not required in this product;

[0033] (5) Take 50 healthy mice and take the concentrated solution of this product orally, which is equivalent to 20 times the dose of normal or...

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Abstract

The invention relates to a method for preparing an anti-Newcastle disease specific transfer factor in vitro. Chicken spleen or peripheral blood is used as a raw material to prepare a lymphocyte monolayer, then phytohemagglutinin and the Newcastle disease are used for inducing and culturing lymphocytes, and the anti-Newcastle disease specific transfer factor is produced in vitro. The prepared specific transfer factor can be used for preventing and treating the Newcastle disease, and meanwhile, the immunity of chickens can be improved. The preparation method has the advantages of high specificity, low cost, high yield, good effect and the like, and is an ideal preparation method of the Newcastle disease transfer factor.

Description

technical field [0001] The invention relates to the field of veterinary medicine, more specifically, to a preparation method of anti-chicken Newcastle disease transfer factor. Background technique [0002] Chicken Newcastle disease (New Castle disease) is a highly contagious infectious disease caused by paramyxovirus. Also known as Asian chicken plague or pseudo-chicken plague. Often showed symptoms of acute sepsis. The main features are dyspnea, loose stools, neurological disturbances, and mucosal and serosal bleeding. High mortality, serious harm to the chicken industry. It was first discovered in Indonesia in 1926, and was soon discovered in Newtown, England. There are popular records all over the world. There are two types of virulent strains and weak virulent strains. The virus is divided into three types: low virulence type (ie slow-onset type), medium virulence type (ie medium-onset type), and virulent type (ie immediate-onset type). Most highly virulent strains...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/57A61P31/14A61P37/04
CPCA61K35/57A61P31/14A61P37/04
Inventor 刘鼎阔刘芳李源
Owner DINGZHENG XINXING BIOTECH TIANJIN
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