Preparation method of anti-Newcastle disease transfer factor

Abstract

The invention relates to a method for preparing an anti-Newcastle disease specific transfer factor in vitro. Chicken spleen or peripheral blood is used as a raw material to prepare a lymphocyte monolayer, then phytohemagglutinin and the Newcastle disease are used for inducing and culturing lymphocytes, and the anti-Newcastle disease specific transfer factor is produced in vitro. The prepared specific transfer factor can be used for preventing and treating the Newcastle disease, and meanwhile, the immunity of chickens can be improved. The preparation method has the advantages of high specificity, low cost, high yield, good effect and the like, and is an ideal preparation method of the Newcastle disease transfer factor.

Description

technical field

[0001] The invention relates to the field of veterinary medicine, more specifically, to a preparation method of anti-chicken Newcastle disease transfer factor. Background technique

[0002] Chicken Newcastle disease (New Castle disease) is a highly contagious infectious disease caused by paramyxovirus. Also known as Asian chicken plague or pseudo-chicken plague. Often showed symptoms of acute sepsis. The main features are dyspnea, loose stools, neurological disturbances, and mucosal and serosal bleeding. High mortality, serious harm to the chicken industry. It was first discovered in Indonesia in 1926, and was soon discovered in Newtown, England. There are popular records all over the world. There are two types of virulent strains and weak virulent strains. The virus is divided into three types: low virulence type (ie slow-onset type), medium virulence type (ie medium-onset type), and virulent type (ie immediate-onset type). Most highly virulent strains...