Polypeptide composition and application thereof
A technology of polypeptide composition and composition, which is applied in the direction of active ingredients, applications, and animal repellents of hydroxyl compounds, which can solve problems such as inability to represent synergistic effects, achieve broad commercial application prospects, save costs, and facilitate transportation
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Embodiment 1
[0039] Example 1 Anti-Escherichia coli Performance Test of Polypeptide and Ethanol Compounding
[0040] Fractional inhibitory concentration (FIC) index is one of the parameters of antibacterial pharmacodynamics (PD), which is the combined drug susceptibility of two antibacterial drugs. Irrelevance and addition of four cases.
[0041] Calculation of FIC index: FIC index=MIC(group A combined) / MIC(group A used alone)+MIC(group B combined) / MIC(group B used alone).
[0042] FIC index interpretation standard: when the FIC index is ≤0.5, the two drugs are synergistic; when the FIC index is 0.5-1, the two drugs are additive; when the FIC index is 1-2, the two drugs are irrelevant Effect; when the FIC index>2, the two drugs are antagonistic.
[0043] Since both peptides and ethanol are antibacterial substances, the FIC index was selected to evaluate the effect of the two compounds.
[0044] Operate according to the provisions of 2.1.8.4 of the Technical Specification for Disinfectio...
Embodiment 2
[0054] Embodiment 2 Polypeptide and ethanol compound anti-Staphylococcus aureus performance test
[0055] Dilute the polypeptide with sterile ultrapure water to the concentration of 0mg / L, 12.5mg / L, 25mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, ethanol with sterile Ultrapure water was diluted to the concentration of 0%, 1%, 3%, 6%, 9%, 12%, 15%, 18%, 21%, 24%, and 50 μL of the above-mentioned different concentrations of polypeptide and ethanol concentration were taken, Put it in a 96-well plate, add 100 μL of Escherichia coli bacterial suspension to each well, and set positive and negative controls, wherein the positive control is 100 μL sterile water + 100 μL bacterial suspension; the negative control is 100 μL sterile water +100 μL sterile (2X) LB medium. The experiment was set up in two parallels, named group 3 and group 4.
[0056] The 96-well plate was placed in a 37±2°C incubator for 48 hours, and the results were observed.
[0057] When the positive control has...
Embodiment 3
[0062] Example 3 Anti-Bacillus subtilis performance test of polypeptide and ethanol
[0063] Operate according to the provisions of 2.1.8.4 of the Technical Specification for Disinfection (2002).
[0064] Preparation of bacterial suspension: take the activated bacterial liquid, adjust the OD value, and make the initial bacterial suspension, so that the OD600 of the Bacillus subtilis bacterial liquid is 0.8, and the corresponding bacterial liquid concentration is about 10. 8 cfu / mL, then diluted with (2X) LB medium to a concentration of 5.0×10 5 -5.0×10 6 cfu / mL as the test bacteria suspension.
[0065] Dilute the polypeptide and ethanol with sterile ultrapure water into test solutions of different concentrations, add 50 μL of each to the 96-well plate, and then add 100 μL of the diluted bacterial suspension.
[0066] Dilute the peptide with sterile ultrapure water to the concentration of 0mg / L, 0.2mg / L, 0.39mg / L, 0.78mg / L, 1.56mg / L, 3.13mg / L, 6.25mg / L, 12.5mg / L. L, 25mg / L,...
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