Application of spore wall protein in prevention and control of enterocytozoon hepatopenaei infection by mixing and feeding shrimps

Abstract

The invention discloses an application of spore wall protein in prevention and control of enterocytozoon hepatopenaei infection in mixed feeding of shrimps, and belongs to the technical field of disease prevention and control in aquaculture. According to the method, the spore wall protein SWP12 of the enterocytozoon hepatopenaei is produced through prokaryotic expression, after the spore wall protein is mixed into feed according to different concentrations to feed prawns, it is found that when the prawns fed with high-concentration protein (65 mg/kg) feed are fed with hepatopancreas containing the enterocytozoon hepatopenaei for at least 10 days after feeding of the SWP12 protein is stopped, the prawns can still resist infection of the enterocytozoon hepatopenaei by being fed with hepatopancreas containing the enterocytozoon hepatopenaei; even within 21 days after the feeding of the SWP12 protein is stopped, the infection rate of the enterocytozoon hepatopenaei is still obviously lower than that of the prawns which are not fed with the SWP12 protein or are fed with the low (medium)-concentration SWP12 protein, so that the capability of the prawns for resisting enterocytozoon hepatopenaei infection can be obviously enhanced by feeding the high-concentration enterocytozoon hepatopenaei spore wall protein SWP12. The method is safer, more effective and more operable.

Description

technical field [0001] The invention belongs to the technical field of aquaculture disease prevention and control, and in particular relates to the preparation of a spore wall protein and its application in the prevention and control of shrimp Enterocytosis hepatis infection in mixed feeding and feeding shrimp. Background technique [0002] Shrimp Enterocytozoon hepatopenaei (abbreviated as EHP) belongs to the order Microsporidia, Enterocytozoonidae, Enterocytozoon, and is a common intracellular parasitic microorganism in the culture of Litopenaeus vannamei. The sporozoites, mainly parasitic in the cells of the hepatopancreas, cause a disease called enteroplasmosis hepatosomiasis. Shrimp suffering from enterocytosis of shrimp mainly showed growth retardation, and its body weight was only 10% to 40% of the body weight of the same batch of healthy shrimp. The time of feeding and listing was prolonged, and the feed consumption was greater than that of the normal shrimp, but the...

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Problems solved by technology

Shrimp suffering from shrimp hepatoenterocytosis mainly shows growth retardation, and its body weight is only 10% to 40% of the body weight of healthy shrimp in the same batch. The time for feeding and marketing is prolonged, and the feed consumption is greater than that of normal shrimp, but the output is higher. It not only consumes more feed, but also reduces the number of shrimps raised in the same pond within a year, causing serious economic losses to

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