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Preparation and application of non-immobilized photoelectric sensor for detecting ATP (adenosine triphosphate)

A sensor, photoelectrochemical technology, applied in the direction of analysis, instrument, measurement device, etc. by causing chemical reaction of materials, can solve the problems of signal interfering substances, complex steps, etc., to achieve good biocompatibility, simple preparation method, toxicity low effect

Pending Publication Date: 2022-07-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, most of the current photoelectrochemical sensors often modify the photoelectric materials layer by layer in order to obtain lower detection limits. This process is not only complicated but also easy to introduce signal interference substances. To avoid these problems, improve the detection sensitivity to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 A preparation method of a non-immobilized photoelectrochemical sensor for detecting ATP, a specific preparation method of a magnetic composite MOFs material encapsulating AA-Fe3O4@ZIF-90@AA:

[0022] 20.0 mg of PAA-functionalized Fe3O4 NPs were first dissolved in 50 mL of 2-ICA DMF solution (0.2 M) by sonication, and then AA was dissolved in the above solution with stirring to obtain Fe3O4 / 2-ICA / AA DMF solution. Then, 50 mL of Zn(OAC)2 DMF solution (0.1 M) was slowly dropped into the Fe3O4 / 2-ICA / AA DMF solution while stirring. After 10 min, the product Fe3O4@ZIF-90@AA was separated with a magnet. And washed three times with ethanol (12 000 rpm, 10 min), and finally re-dispersed in 100 mL of 0.1M Na2SO4 solution;

[0023] The AA was dissolved in the above solution under stirring, and the amount of AA added was 1.5 g.

Embodiment 2

[0024] Example 2 A non-immobilized photoelectrochemical sensor for detecting ATP is applied to the detection of ATP, and its specific detection method is:

[0025] (1) Add 50 μL of ATP standard solution (0.00, 1.00, 5.00, 10.0, 20.0, 40.0, 60.0, 80.0, 100, 120 mM) to 10 mL of the above dispersion of Fe3O4@ZIF-90@AA at 37 °C After 10 min of reaction, the released AA was quickly separated with a magnet, and 8 mL of the solution containing AA was transferred to the electrolytic cell with a pipette;

[0026] The electrolytic cell contains 15 mL of Na2SO4 (0.1M) solution;

[0027] (2) The working electrode W1, the platinum wire electrode as CE, and the Ag / AgCl reference electrode RE were inserted into the above-mentioned AA-added electrolyte to construct a three-electrode system. In a dark environment, a 300W Xe lamp was used as the excitation light source. The electrochemical workstation measures the photoelectric signal, applies a constant voltage to W1 by the time-current metho...

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Abstract

The invention discloses a preparation method and application of a non-immobilized photoelectrochemical sensor for detecting ATP (adenosine triphosphate), and belongs to the technical field of photoelectric biosensing analysis. The method comprises the following steps: synthesis of a magnetic MOFs material for packaging AA, namely Fe3O4 ZIF-90 AA, construction of a photoelectric sensor system, and photoelectrochemical analysis and detection of adenosine triphosphate (ATP). The method has the characteristics that a photoelectrochemical biological analysis method with the advantages of simple equipment, high response speed, high sensitivity and the like is combined with a specific response mechanism between the ATP and Zn < 2 + > in the ZIF-90, so that the dependence on a nucleic acid aptamer is eliminated, the interference of layer-by-layer modification on an electrode is avoided, and finally, the non-immobilized sensitive detection of the ATP is realized.

Description

technical field [0001] The invention relates to a preparation method of a non-immobilized photoelectrochemical sensor for detecting ATP. 2+ specific response mechanisms. It belongs to the technical field of photoelectric biosensing analysis. Background technique [0002] Adenosine triphosphate (ATP) is a nucleotide that acts as a "molecular currency" for energy transfer within cells, storing and transmitting chemical energy. It plays an important role in regulating cell metabolism, physiology and biochemistry. ATP is also an indispensable signaling molecule in many biological processes, and its abnormal levels are related to the occurrence of various diseases, such as pancreas, heart, vascular diseases, Alzheimer's disease, Parkinson's disease, hypoxia, hypoglycemia and some malignant diseases. tumor. The development of a reliable, sensitive, and selective assay for ATP content will help assess conditions such as cell damage, cell inhibition, cell proliferation, and micr...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N21/75C08G83/00
CPCG01N27/305G01N27/3275G01N27/3278G01N27/48G01N21/75C08G83/008Y02P20/133
Inventor 颜梅李成芳张晶郝梦娇魏全勇薄怡然
Owner UNIV OF JINAN
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