Preparation and detection method of two-channel visual multicolor fluorescent probe
A technology of fluorescent probes and detection methods, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve problems such as reducing sensitivity and selectivity
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Embodiment 1
[0041] A preparation method of a dual-channel visualization multicolor fluorescent probe is as follows:
[0042] 1. Amination modification of halloysite nanotubes
[0043]The manner of amination modification of halloysite nanotubes is as follows. First, 1 g of halloysite nanotubes were dispersed in 50 mL of xylene, sonicated for 15 minutes, and stirred at room temperature for 1 hour to make the solution more uniformly dispersed. 5mL of silane coupling agent 3-chloropropyltrimethoxysilane was added dropwise to the above solution, stirred at room temperature for 10 minutes, the mixture was refluxed in an oil bath at 80°C for 8h, passed through the silane coupling agent and halloysite. Coupling of silanol groups on the nanotube surface introduces chloropropyl groups to the halloysite nanotube surface. The supernatant was removed by centrifugation (7000 rmp, 3 min), washed with ethanol three times to remove unreacted silane coupling agent, and vacuum dried at 60° C. for 5 hours....
Embodiment 2
[0049] Detect DPA
[0050] Dissolve 2,6-pyridinedicarboxylic acid (DPA) in deionized water, prepare a certain concentration of DPA solution (0-81 μM), take 100 μL (1 mg / mL) of fluorescent nanocomposite probe with Tris-HCl (pH=8) Buffer to 2mL. After adding different concentrations of DPA, the sensitivity of HNT@CDs-MOF fluorescent nanoprobe to identify DPA was investigated under the excitation wavelength Ex=280nm channel. After adding DPA, the fluorescence intensity of the fluorescent nanoprobe at 545nm was significantly enhanced, and the red fluorescence at 616nm did not change significantly, such as figure 1 shown. In the range of 0-81 μM, with the increase of DPA concentration, the fluorescence change from red to green is realized. The concentration of DPA has a good linear relationship with the fluorescence intensity, and the correlation coefficient R 2 =0.99784, the associated linear equation is I 545 / I 616 =3.54072C DPA +0.44552, the detection limit was as low as ...
Embodiment 3
[0053] Detect TC
[0054] Dissolve tetracycline (TC) in deionized water, prepare a TC solution with a certain concentration, take 100 μL (1 mg / mL) of fluorescent nanocomposite probe with Tris-HCl (pH=9) buffer and dilute to 2 mL, and add a certain amount of TC (0-19μM), measured the fluorescence emission spectrum under the excitation wavelength Ex=370nm channel, the emission color changed from blue to red, the fluorescence intensity of the system at 616nm increased with the increase of TC, and the fluorescence intensity at 450nm decreased. . With the increase of TC concentration, the blue fluorescence of HNT@CDs-MOF nanosensor slightly decreased at 450 nm, and the red fluorescence intensity increased significantly at 616 nm, as shown in figure 2 shown.
[0055] In the range of 0-6 μM and 6-19 μM, the concentration of TC showed a good linear relationship with the fluorescence intensity, R 1 2 = 0.99761 and R 2 2 =0.98038, the related linear equation can be expressed as I...
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