Application of lactobacillus rhamnosus strain LRa05 in preparation of immunity enhancing product and/or eczema relieving product
A technology of Lactobacillus rhamnosus and enhancing immunity, which is applied in the field of microorganisms and can solve the problem of small selection space
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experiment example 2
[0061] The control strain Lactobacillus rhamnosus LGG strain used in Experimental Example 2 was purchased from the Guangdong Microorganism Culture Collection;
[0062] Escherichia coli, Staphylococcus aureus, and Salmonella used in Experimental Examples 6-7 were purchased from Guangdong Microorganism Culture Collection Center.
[0063] The mice used in Experimental Examples 8-9 were purchased from the Animal Center of Huazhong Agricultural University.
[0064] The first group of examples, the use of the product of the strain LRa05 of the present invention
[0065] The examples in this group provide the use of Lactobaccillus rhamnosus strain LRa05 with a deposit number of CGMCC No. 24377 in the preparation of immunity-enhancing products and / or eczema-relieving products.
[0066] Any use, preparation, production, sale, offering for sale, cultivation, propagation, fermentation of Lactobaccillus rhamnosus strain LRa05 with the deposit number of CGMCC No. 24377 and its use in the ...
experiment example 1
[0102] Experimental example 1. Isolation, screening and identification of Lactobacillus rhamnosus
[0103] (1) Isolation and screening of strains
[0104] Collect fecal samples from healthy humans, take 1 g to 9 mL of sterile saline, fully shake and disperse the samples, take 100 μL of samples for gradient dilution, select an appropriate gradient and evenly spread them on MRS agar plates, place them under anaerobic conditions, and incubate at 37 ° C for 36 After ~48h, a single suspected single colony was selected, gram staining was performed, and the morphological characteristics of the colony were observed by microscopy, and the strains suspected of Lactobacillus were initially screened. The strain was repeatedly streaked and purified on MRS solid medium to obtain a purified strain of Lactobacillus, and then the purified strain was stored in a -80°C glycerol tube.
[0105] MRS medium formula (g / L): peptone 10, beef extract 5, yeast powder 5, glucose 20, anhydrous sodium acet...
experiment example 3
[0120] Experimental Example 3. Comparison Experiment of Lactobacillus rhamnosus Fermentation Tank Enlargement
[0121] Seed culture: insert Lactobacillus rhamnosus into 10 mL of seed medium, and stand at 37°C for 6-8 hours to be the first-class seeds. In 200mL seed medium, 37 ℃ static culture for 5-6h is the secondary seed.
[0122] Fermentation tank culture: the volume of the fermenter is 15L, 10L of fermentation medium is put into it, sterilized at 115°C for 20min, and all the secondary seeds obtained from the culture are inoculated into the fermenter with an inoculation amount of 2% (v / v). Fermentation was started under the conditions of 35°C, stirring speed of 100 rpm, and initial pH of 6.5. During the fermentation, 23% sodium carbonate was used to maintain the pH at 5.8, and nitrogen was introduced to maintain the tank pressure at 0.03 MPa for 10 hours. The number of viable bacteria in the obtained fermentation broth is shown in Table 2 below.
[0123] The formula of th...
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