Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application

A technology of activated sludge and ammonia oxidizing bacteria, which is applied in chemical instruments and methods, water/sludge/sewage treatment, water pollutants, etc., to shorten the enrichment time and improve the enrichment effect.

Pending Publication Date: 2022-07-29
NORTH CHINA ELECTRIC POWER UNIV (BAODING)
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although complete ammonia oxidizing bacteria have these advantages, rapid enrichment of complete ammonia oxidizing bacteria in activated sludge is still a key point and difficulty faced by current research on complete ammonia oxidizing bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application
  • Device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application
  • Device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Construction of enrichment reaction system

[0031]First, all the 10L activated sludge taken from Xiaojiahe Sewage Plant was inoculated into the main reactor, and then the culture medium in the water inlet bucket entered the main reactor through the water inlet pipe under the action of the peristaltic pump, so that the medium was filled from bottom to top Complete the main reactor until the medium reaches the level where the overflow is located. At the same time, 15% of polyurethane fillers and 15% of Kaldness No. 3 were added to the main reactor, wherein the polyurethane fillers were purchased from Qingdao Banghao Environmental Technology Co., Ltd., and Kaldness No. 3 was purchased from Yixing Jushunfu Environmental Protection Equipment Co., Ltd., two The specific properties of the fillers are shown in Table 1. Then, turn on the water outlet peristaltic pump, so that the water in the main reactor is pumped to the water outlet pipe through the membrane module...

Embodiment 2

[0044] Embodiment 2 The detection of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen concentration of in and out water samples

[0045] Starting from the operation of the main reactor, the 0d, 5d, 10d, 15d, 25d and 30d samples were taken from the outlet pipe respectively, according to Nessler's reagent colorimetry, N-(1-naphthyl)-ethanedi Amine spectrophotometry and ultraviolet spectrophotometry were used to measure ammonia nitrogen, nitrite nitrogen and nitrate nitrogen concentrations in the water samples in and out, respectively. The results are as follows: Figures 2 to 4 shown. At the same time, the ammonia oxidation rate in the main reactor was calculated to judge the growth of microorganisms in the reactor.

[0046] Depend on Figures 2 to 4 It can be seen that: in general, the effect of the reactor is stable during the operation period. The ammonia nitrogen in the influent is all converted into nitrate nitrogen through nitrification, and no accumulation of nit...

Embodiment 3

[0047] Embodiment 3 Complete ammonia oxidizing bacteria accounts for the detection of all ammonia oxidizing microorganism gene Copy numbers ratio

[0048] On the 10th, 20th and 30th days of main reactor operation, activated sludge and biological samples on Kaldnes No. 3, polyurethane sponges were collected. Genomic DNA from each sample was extracted using the FastDNA kit (MP Biomedical, Illkirch, France) according to the manufacturer's instructions. The amoA genes in Comammox, AOA and AOB were amplified by q-PCR using specific primers amoAAF / SR, amoA 1F / 2R and archamoA 19F / 616R on the AppliedBiosystems 7300 system, respectively. The base sequences are shown in Table 2. q-PCR amplification reactions were performed in 20uL pre-reaction mix containing 10 μL of 2 × Super Real Premix Plus, 0.5 μL of forward and reverse primers, 2 μL of template DNA, and 5 μL of ribonuclease-free ddH 2 O. Before using the standard curve, the recovered PCR product has been ligated into the pMD18-T ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
PCR efficiencyaaaaaaaaaa
Login to View More

Abstract

The invention provides a device and method for rapidly enriching complete ammonia oxidizing bacteria from activated sludge and application, and belongs to the technical field of feed water treatment. The device mainly comprises a main reactor, a water inlet bucket, a waste liquid bucket, a water inlet peristaltic pump, a water outlet peristaltic pump, a water inlet pipe, a water outlet pipe, a stirring motor, polyurethane filler, Kaldness3 and a membrane assembly. According to the method, the activated sludge is cultured in a continuous water inlet and outlet mode, ammonium chloride is used as the only ammonia nitrogen source, water is fed under the ammonia nitrogen oligotrophic condition, the optimal culture medium and filler are used in a matched mode, the q-PCR detection means is adopted, and finally the highest proportion of complete ammonia-oxidizing bacteria in the Copy number of all ammonia-oxidizing microorganism genes is obtained. The beneficial effect of quickly enriching the complete ammonia oxidizing bacteria from the activated sludge is achieved. According to the method, the enrichment time of the complete ammonia oxidizing bacteria is greatly shortened, the enrichment effect of the complete ammonia oxidizing bacteria is comprehensively improved, and the method has the advantages of accuracy, high efficiency, rapidness and the like.

Description

technical field [0001] The invention belongs to the technical field of water supply treatment, and in particular relates to a device, method and application for rapidly enriching complete ammonia oxidizing bacteria from activated sludge. Background technique [0002] Nitrogen cycle is an important part of the cycle of elements in nature, and it is of great significance to study its cycle mode and cycle characteristics to maintain the ecological balance of nature and remove nitrogen-containing pollutants in sewage plants. As the rate-limiting step of nitrogen cycle, nitrification is generally considered to require two steps to complete, that is, ammonia nitrogen in water first passes through ammonia-oxidizing bacteria (AOB) or / and ammonia-oxidizing archaea in the presence of dissolved oxygen. (ammonia-oxidizingarchaea, AOA) is converted into nitrous nitrogen (NH 3 / NH 4 + →NO 2 - ), and then converted into nitrate by the action of nitrite-oxidizing bacteria (NOB). Until...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/12C02F101/16
CPCC02F3/1268C02F2101/16
Inventor 郑茂盛李云龙黄淑君牟广利顾瑷璐
Owner NORTH CHINA ELECTRIC POWER UNIV (BAODING)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com