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Copolymerized polystyrene fluorescent microsphere and preparation method thereof

A technology of fluorescent microspheres and polystyrene, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of fluorescence effect attenuation, environmental pollution, active site occupation, etc., and achieve stable fluorescence performance, good monodispersity, Good fluorescent performance

Active Publication Date: 2022-08-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common preparation methods of fluorescent microspheres include physical adsorption method, embedding method, swelling method, copolymerization method, etc. The fluorescent microspheres prepared by physical adsorption method are unstable, and the fluorescent substances adsorbed on the surface of the microspheres are easy to fall off, and the surface of the microspheres Active sites occupied by fluorescent substances are not conducive to subsequent biological applications
The preparation process of the swelling method is simple, but the use of organic solvents is involved in the preparation process, which pollutes the environment
Secondly, the fluorescent substance inside the microsphere is unstable during storage and is prone to overflow, and because the fluorescent substance has a certain solubility in the solvent, the overflowing fluorescent substance dissolves, resulting in attenuation of the fluorescence effect

Method used

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  • Copolymerized polystyrene fluorescent microsphere and preparation method thereof
  • Copolymerized polystyrene fluorescent microsphere and preparation method thereof
  • Copolymerized polystyrene fluorescent microsphere and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0029] 1) Styrene refining:

[0030] Take an appropriate amount of basic alumina and put it in a chromatography column, and 100 mL of styrene is used to remove the polymerization inhibitor in the chromatography column. Put the treated styrene into a dry reagent bottle, and add an appropriate amount of molecular sieve to remove possible residual moisture. Styrene was stored in a 4°C refrigerator.

[0031] 2) Azobisisobutyronitrile purification:

Embodiment 2

[0033] The invention provides a copolymerized fluorescent microsphere and a preparation method thereof. The preparation raw materials include: 86.71% of styrene, 1.73% of azobisisobutyronitrile (AIBN), 7.95% of polyvinylpyrrolidone (PVP), anhydrous ethanol: Distilled water = 9:1, rare earth europium complex (Eu(BFA) 3 (Aphen)) 3.61%, the preparation method comprises the following steps:

[0034] 1) Add a certain amount of PVP, anhydrous ethanol and distilled water to the three-necked flask, stir evenly, pass nitrogen to empty for 30min, slowly add AIBN-dissolved anhydrous ethanol solution dropwise, continue stirring and then add styrene dropwise.

[0035] 2) Then slowly dropwise add Eu(BFA) dissolved in it 3 (Aphen) complex in anhydrous ethanol solution, the process has been N 2 Atmosphere protection, after stirring evenly, heated to 70 ℃.

[0036] 3) Keep a certain stirring speed and react for 12h. A milky white liquid was obtained, the reaction was stopped by centrifugat...

Embodiment 3

[0040] The preparation raw materials include: 87.34% styrene, 1.75% azobisisobutyronitrile (AIBN), 8.01% polyvinylpyrrolidone (PVP), absolute ethanol:distilled water=9:1, rare earth europium complex (Eu(BFA) 3 (Aphen)) 2.91%, the preparation method comprises the following steps:

[0041] 1) Add a certain amount of PVP, anhydrous ethanol and distilled water to the three-necked flask, stir evenly, pass nitrogen to empty for 30min, slowly add AIBN-dissolved anhydrous ethanol solution dropwise, continue stirring and then add styrene dropwise.

[0042] 2) Then slowly dropwise add Eu(BFA) dissolved in it 3 (Aphen) complex in anhydrous ethanol solution, the process has been N 2 Atmosphere protection, after stirring evenly, heat to 70 ℃

[0043] 3) Keep a certain stirring speed and react for 12h. A milky white liquid was obtained, the reaction was stopped and centrifuged with a centrifuge, the supernatant was discarded, washed with distilled water and then centrifuged, washed with ...

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Abstract

The invention discloses a copolymerized polystyrene fluorescent microsphere and a preparation method thereof, a fluorescent substance is a rare earth europium complex, and the rare earth europium complex takes a phenanthroline derivative introduced with double bonds as a second ligand and beta-naphthoyl trifluoroacetone as a first ligand. The rare earth europium complex and styrene are initiated by an initiator in a solvent, the fluorescent microspheres are obtained through free radical copolymerization, and the content of the europium complex in the fluorescent microspheres is regulated and controlled to obtain the optimal fluorescence performance. The copolymerized fluorescent microspheres disclosed by the invention have the advantages of good dispersity, uniform particle size, good luminous effect, high fluorescence intensity, long fluorescence lifetime and the like. The copolymerization type fluorescent microspheres are good in stability, overflow of internal fluorescent substances is avoided, and technical support is provided for application of the fluorescent microspheres in the fields of immunoassay, standard substance biological probes and the like.

Description

technical field [0001] The invention relates to a method for preparing fluorescent microspheres and, in particular, to a copolymerized polystyrene fluorescent microsphere and a preparation method thereof. Background technique [0002] In recent years, fluorescent microspheres based on rare earth complexes have been widely used in biology, medicine, detection and other fields. The main application directions are high-throughput drug screening, tracer imaging of biological cells, immunoassay, flow cytometry of biological cells, etc. Rare earth europium complexes have strong characteristic fluorescence, good chemical stability, long excitation lifetime and high theoretical quantum efficiency, and are often used in microspheres as luminescent centers. Research. [0003] The invention patent publication number CN101177607 discloses a copolymerized cross-linked polymer fluorescent microsphere and a preparation method thereof. The 8-hydroxyquinoline metal complex is used as the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F212/08C08F220/60C09K11/06
CPCC08F212/08C09K11/06C09K2211/182C09K2211/1466C08F220/606
Inventor 孙伯旺刘敏蔡卓尔徐杨侯煜淋
Owner SOUTHEAST UNIV
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