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Preparation method of debaryomyces hansenii freeze-dried microbial inoculum and application of debaryomyces hansenii freeze-dried microbial inoculum in controlling strawberry postharvest soft rot

A dry fungus and yeast technology, applied in the field of biological control, can solve the problems of less antagonistic yeasts and the need to improve the protection effect, and achieve the effects of strong antagonistic effect, good control effect, and avoiding potential harm to the human body and the environment

Pending Publication Date: 2022-08-05
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common lyoprotectants include sugars, proteins, antioxidants and amino acids, etc., but the protective effect needs to be improved
Moreover, the current research on the commercial application of antagonistic bacteria in the field of biological control of postharvest diseases of fruits and vegetables mainly focuses on antagonistic bacteria, and there are few reports on antagonistic yeasts.

Method used

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  • Preparation method of debaryomyces hansenii freeze-dried microbial inoculum and application of debaryomyces hansenii freeze-dried microbial inoculum in controlling strawberry postharvest soft rot
  • Preparation method of debaryomyces hansenii freeze-dried microbial inoculum and application of debaryomyces hansenii freeze-dried microbial inoculum in controlling strawberry postharvest soft rot
  • Preparation method of debaryomyces hansenii freeze-dried microbial inoculum and application of debaryomyces hansenii freeze-dried microbial inoculum in controlling strawberry postharvest soft rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Inoculate Hamson Dbaril yeast in the NYDB medium, at a temperature of 28 ° C and a speed of 180rpm, it is activated and cultivated for 24h to obtain the activated medium. After the body precipitation, the bacterial precipitation is washed twice with a sterile water, and the concentration is prepared with a sterile water to be 1 × 10 8 Cells / ml's yeast suspension;

[0063] (2) Add seaweed sugar, dehydrated milk, L-anti-hemoglobin and sodium L-glutamate to dissolve in sterile water to obtain a protective agent solution; the quality concentration of seaweed sugar in the protective agent solution is 2.22 %, and the quality concentration of dehydrated milk quality concentration It is 17.83 %, the mass concentration of L-anti-severic acid is 0.06 %, and the mass concentration of sodium L-glutamate is 4.06 %;

[0064] (3) Mix the protective solution of the yeast suspension with steps (2) of the step (1) (2). , Get a pre-frozen mixture; freeze the pre-frozen mixture for freezin...

Embodiment 2

[0070] Hamson Dbaril yeast frozen dry bacteria control effect on strawberries after being stored during storage

[0071] (1) Test plan

[0072] The preparation method of Hamson Dbarbari yeast frozen dry bacteria is the same as above. Hamson Dbaril yeast frozen dry bacteria at 28 ° C in the NYDB medium, 180rpm activation and culture for 24h. After the activation is completed, the strain fermentation solution is 4 ° C. The bacterial saline was cleaned twice under the same centrifugation conditions, and then the concentration of yeast concentration was adjusted with sterile physiological saline to 1 × 10 8 Cells / ml spare.

[0073] Place the selected strawberry fruit in a clean plastic basket for later use, use a sterile pitch to play a 3mm diameter and 3mm deep wound at the strawberry equator, add 30 μl of yeast suspension each wound, and add the volume of the volume Sterile physiological saline is used as a blank control; the strawberry fruit after treatment is naturally dried and t...

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Abstract

The invention belongs to the technical field of biological control, and particularly relates to a preparation method of a debaryomyces hansenii lyophilized fungicide and application of the debaryomyces hansenii lyophilized fungicide in controlling strawberry postharvest soft rot. Antagonistic yeast debaryomyces hansenii is used as a research object, a protective agent is creatively screened, a freeze drying technology is used for preparing the yeast preparation, and the control effect of the yeast preparation on the soft rot of the strawberries after picking is researched. The optimal protective agent scheme for freeze drying of the D.hansenii preparation is as follows: 2.22% of trehalose, 17.83% of skimmed milk powder, 0.06% of L-ascorbic acid and 4.06% of L-sodium glutamate; the D.hansenii freeze-dried preparation obtained by an optimal compounding scheme can keep a relatively high survival rate in a relatively long storage period, and has a remarkable control effect on the soft rot of the picked strawberries; the method is convenient to operate, safe and environment-friendly, and has good economic and social benefits.

Description

Technical field [0001] The present invention is a biological control technology field, which involves the preparation method of Hamson Dbaril yeast frozen dry bacteria and its use of soft corruption after controlling strawberry. Background technique [0002] Strawberries, also known as forerighs, ground fruits, scho -pear, etc., are perennial herbaceous plants. The fruits are delicious and juicy, rosy and seductive in color, and have a pleasant special fruit aroma. The name of "Queen of Fruit" around the world has been widely loved by consumers. However, strawberry fruits are delicate, soft, and thin. After picking, they are easily affected by mechanical damage and bad environment during their storage, transportation and sales. After the opportunity, the strawberry fruit eventually caused a fungal disease after harvesting. In the deterioration of strawberries caused by such diseases, soft rot diseases caused by Rhizopus Stolonifer are particularly serious. The occurrence of soft ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/04A23B7/155C12R1/645
CPCC12N1/16C12N1/04A23B7/155
Inventor 赵利娜兰晨张红印张晓云杨其亚王凯利
Owner JIANGSU UNIV