Method for regulating and controlling conduction velocity of tissue-engineered conduction bundle and application

Abstract

The invention provides a method for regulating and controlling the conduction velocity of a tissue-engineered conduction bundle and application. By changing the cell planting density and the tissue culture period in the tissue engineering heart conduction bundle construction and culture process, it is found that the conduction speed is regulated and controlled most effectively when the planting density is 1 * 10 < 7 >-1.1 * 10 < 7 > cells / ml and the culture period is 2 weeks. On the basis of the parameters, heart cells overexpressing Tbx3 and common heart progenitor cells are jointly planted on a collagen sponge stent to construct ECT, and it is found that the effect of precise regulation and control of the conduction velocity is better through the matching of 70-80% of Tbx3 + H9C2 cells and 20-30% of Tbx3-H9C2 cells. The conduction velocity of the ECT constructed by the method is close to the in-vivo atrioventricular junction level, the key problem of realizing physiological atrioventricular electrical conduction in the process of reconstructing an atrioventricular pathway through a tissue engineering conduction bundle (ECT) is solved, namely, the problem of how to ensure atrioventricular extension after ECT transplantation is solved, and an effective method is provided for regulating and controlling the conduction velocity of the ECT.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a method for regulating the conduction velocity of a tissue-engineered conduction bundle for treating heart atrioventricular block disease and a tissue-engineered conduction bundle constructed by the method. Background technique [0002] Severe atrioventricular block is a common complication of various diseases in cardiac surgery, which seriously endangers human health. At present, it is mainly treated by implanted electronic pacemaker. Electronic pacemakers can improve the symptoms of patients, but due to their mechanical structure, there are many problems such as susceptibility to electromagnetic interference, lack of neurohumoral regulation, electrode infection, battery replacement, and inability to develop with random body development. In view of the insufficiency of electronic pacemakers, engineered conduction tract or tissue (ECT), as a new idea for the treat...

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Problems solved by technology

Bakker et al. further overexpressed Tbx3 in cardiomyocytes in vivo and in vitro, and found that Tbx3 reset the gene program of mature cardiomyocytes, inhibited the expression of fast conduction channels such as Connexin43, Connexin40, Nav1. Martijn LBakker, Gerard J J Boink, Bas J Boukens, et al. T-box transcription factor TBX3 reprogrammes mature cardiac myocytes into pacemaker-like cells. Cardiovasc Res. 2012 Jun 1; 94(3):439-49.)

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