O-type foot and mouth disease virus strain with improved replication titer as well as construction method and application of O-type foot and mouth disease virus strain

A technology of foot-and-mouth disease virus and construction method, which is applied in the field of O-type foot-and-mouth disease virus strain and its construction, can solve the problems of long time for virus collection, low replication titer, unsuitability for large-scale production of FMD vaccine, etc.
CN114854698AActive Publication Date: 2022-08-05LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
1 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2022-08-05

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides an O-type foot and mouth disease virus strain with improved replication titer as well as a construction method and application thereof, and belongs to the technical field of biological products. Through a reverse genetic manipulation technology of the foot and mouth disease virus, a G-H ring gene of the foot and mouth disease virus O / NXYCh / CHA / 2018 strain is further used for replacing a corresponding gene on a recombinant virus skeleton of a main immune gene of the chimeric foot and mouth disease virus O / XJ / CHA / 2017 strain, the time of causing 100% infected cell lesion by the constructed recombinant virus rHN / XJ / NXGH is remarkably shortened to 12 h, and the time of causing 100% infected cell lesion by the recombinant virus rHN / XJ / NXGH is remarkably shortened to 12 h. And the replication titer of infected cells for 12 hours is improved by more than 5 times. The recombinant virus is continuous in passage and good in hereditary stability, and replacement of the G-H ring does not affect immunogenicity of the vaccine. Therefore, the recombinant foot-and-mouth disease virus strain rHN / XJ / NXGH provided by the invention has the potential to be used as a vaccine candidate strain for effectively preventing and controlling the O-type foot-and-mouth disease in China.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biological products, and in particular relates to an O-type foot-and-mouth disease virus strain with improved replication titer and a construction method and application thereof. Background technique

[0002] Foot-and-Mouth Disease (FMD) is a severe infectious disease caused by Foot-and-Mouth Disease Virus (FMDV), which infects more than 70 kinds of cloven-hoofed animals such as pigs, cattle and sheep. The disease spreads rapidly, is highly contagious, and has a very high incidence rate. The World Organization for Animal Health (OIE) has listed it as a must-reportable animal disease. The outbreak and prevalence of FMD seriously endanger the productivity of livestock and the quality of livestock products, affect the international trade of livestock and their products, and cause huge economic losses to the livestock breeding industry in the affected areas. Therefore, the effective prevention and control of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More