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Method and device of solid-state fermentation by using porous adsorption carrier

A porous adsorption and solid-state fermentation technology, applied in the field of fermentation industry, can solve the problems of difficult application of fermentation products with high purity requirements, and achieve the effect of large-scale sterile inoculation, low equipment investment, and favorable gas-liquid-solid transfer

Inactive Publication Date: 2005-06-22
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a porous adsorption carrier solid-state fermentation method in order to solve the problems existing in the current solid-state fermentation tank that it is difficult to apply to fermented products with high purity requirements.

Method used

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  • Method and device of solid-state fermentation by using porous adsorption carrier
  • Method and device of solid-state fermentation by using porous adsorption carrier
  • Method and device of solid-state fermentation by using porous adsorption carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1: Porous adsorption carrier solid-state fermentation technology clavulanic acid

[0041] In the porous adsorption carrier cylinder 7 in the horizontal solid-state fermentation sealed tank body 5, pack the pulp porous adsorption carrier and its soluble medium (solid-liquid ratio is 1: 2.5), heat up to 120-125 ° C for 20-30 minutes, Turn on the circulating fan 4, after the culture medium is cooled to 40°C, the seed liquid in the liquid seed tank (relative to the 5% seed liquid of the culture medium weight) is inserted into the porous adsorption carrier by the Venturi tube 2, and the porous carrier is controlled. The temperature in the tank is 25--28 ℃, the humidity is 85%--95%, and the fermentation is carried out; during the solid-state fermentation process, a certain humidity in the tank is controlled by an ultrasonic atomizer, and the sterile air ventilation rate entering the tank is 1.0vvm ( The amount of air that passes into the tank body per minute is 1.0 ti...

Embodiment 2

[0051] Embodiment 2: Porous adsorption carrier solid-state fermentation technology genetically engineered bacteria alkaline protease

[0052] Its operation process is with embodiment 1;

[0053] Bacteria: Bacillus subtilis PG1723-3;

[0054] Porous adsorption carrier: sponge;

[0055] Medium: soluble starch 0.8%, tryptone 0.2%, lactose 0.2%, kanamycin 0.005%, calcium chloride 0.02%, pH 10.5;

[0056] Carrier (solid phase) to medium (liquid phase) ratio: 1:1.5;

[0057] Fermentation temperature: 28--32℃

[0058] Liquid inoculum volume: 10% (compared to solid);

[0059] Fermentation period: 3--4 days

[0060] Fermentation titer: 12160-12350IU / g (solid phase);

[0061] Purity of fermentation extract: 45--55%.

Embodiment 3

[0062] Example 3: Porous adsorption carrier solid-state fermentation technology genetically engineered bacteria high-density lipoprotein drug

[0063] Its operation process is with embodiment 1;

[0064] Strains: Pichia pastoris YAC;

[0065] Porous adsorption carrier: sponge or vermiculite;

[0066] Medium: 0.8% methanol, 12.5% ​​glycerol, 0.1% oleic acid, 0.1% histidine, 1.0% ammonium sulfate, pH5.5;

[0067] Carrier (solid phase) to medium (liquid phase) ratio: 1:1--1.5;

[0068] Fermentation temperature: 25--27℃

[0069] Liquid inoculum volume: 10% (compared to solid);

[0070] Fermentation period: 3--4 days

[0071] Fermentation titer: 1.12g / g (solid phase);

[0072] Purity of fermentation extract: 45--50%.

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Abstract

The invention relates to a solid-state fermentation method and device of a porous adsorption carrier; the method adopts a porous adsorption material as the carrier of the medium in the solid-state fermentation on the basis of the traditional horizontal solid-state fermentation; Separation of products; its device is also based on a traditional horizontal fermentation tank with a porous adsorption carrier cylinder; the present invention uses a porous adsorption inert carrier to completely avoid impurities in the solid from being brought into the fermentation extract; due to the porous adsorption carrier Solid-state fermentation in the absence of free water is beneficial to gas-liquid-solid transfer, the fermentation level is 3-5 times higher than liquid fermentation, and the equipment investment is much lower than that of liquid submerged fermentation; the medium is eliminated, liquid seeds are in-situ aseptically inoculated and The supporting aseptic operation methods such as on-site ultrasonic humidity control solve the problems of difficult sterilization of large-scale solid medium and large-scale aseptic inoculation; the invention will broaden the application range of solid fermentation, and has wide applicability and promotion significance.

Description

technical field [0001] The invention belongs to the technical field of fermentation industry, and in particular relates to a porous adsorption carrier solid-state fermentation technology and a device thereof. Background technique [0002] Solid-state fermentation is one of the longest-established technologies for humans to use microorganisms to produce products. It has been used by humans to make various traditional foods thousands of years ago. In recent years, the research on solid-state fermentation in the fields of organic acids, alcohol, antibiotics, genetic recombinant bacteria fermentation drugs and other fields has been rapidly developed and applied, and the application of solid-state fermentation has great potential. [0003] Solid-state fermentation generally refers to the fermentation process of one or more microorganisms in a state where the medium is solid and there is no or almost no free-flowing water. Solid-state fermentation has the following characteristic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/16C12P1/00
Inventor 陈洪章李春李佐虎
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI