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Magnetic compound microsphere of surface blot gel for biological macromolecular template and its reverse-phase suspension polymerization for preparing its seed

A technology of magnetic composite microspheres and biomacromolecules, which is applied in the fields of material science and engineering and bioseparation engineering, can solve the problems of being expensive and unable to elute, and achieve the effects of rapid separation, easy elution, and convenient preparation

Inactive Publication Date: 2005-11-09
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to combine template imprinting technology with magnetic sensitivity through seed reversed-phase suspension polymerization method to prepare non-rigid biomacromolecule template surface imprinted gel magnetic composite microspheres (abbreviated as surface imprinted gel composite microspheres). And endow it with a certain magnetic responsiveness, so that the prepared surface imprinted gel composite microspheres can be easily separated under the action of an external magnetic field after the "active" adsorption and specific recognition of the template biomacromolecule is completed. , to achieve the purpose of active recognition and convenient separation; at the same time, it solves the problem that some template molecules cannot be eluted because some template molecules are embedded in the microspheres in the reversed-phase suspension method, and saves the amount of expensive biological macromolecular templates

Method used

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  • Magnetic compound microsphere of surface blot gel for biological macromolecular template and its reverse-phase suspension polymerization for preparing its seed
  • Magnetic compound microsphere of surface blot gel for biological macromolecular template and its reverse-phase suspension polymerization for preparing its seed
  • Magnetic compound microsphere of surface blot gel for biological macromolecular template and its reverse-phase suspension polymerization for preparing its seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Preparation of bovine serum albumin (BSA) template surface imprinting gel composite microspheres

[0045] (1) Preparation of bulbs

[0046] Put 100 ml of toluene into a 250 ml three-necked flask with a stirrer and a vent tube, add 0.2 g of ethyl cellulose, and stir to dissolve it. Add 1g of methacrylic acid to 11.6ml of 1mol / L NaOH solution, stir for 5min, add 8gAM, 1g Bis and 20ml of water, add 0.2g of ground magnetic Fe after dissolution 3 O 4 The powder is stirred and dispersed evenly and then added to the three-necked flask, and the stirring is continued; 0.1g of potassium persulfate and 0.05g of sodium bisulfite are dissolved in 10ml of water and then added dropwise to the three-necked flask. Deoxidize with nitrogen and react at room temperature for 2h. After the reaction is over, filter out the organic solvent part with a 180-mesh screen, then wash the gel microspheres with acetone, wash repeatedly with water, and soak in 50ml of 1mol / L hydrochloric acid so...

Embodiment 2

[0052] Example 2. Preparation of Lysozyme Surface Imprinting Gel Composite Microspheres

[0053] (1) Preparation of bulbs

[0054] Put 100ml of toluene into a 250ml three-necked flask with a stirrer and a vent tube, add 0.25g of ethyl cellulose, and stir to dissolve it. Add 2g of methacrylic acid to 23.2ml of 1mol / L NaOH solution, stir for 5min, add 7gAM, 1g Bis and 10ml of water, add 0.3g of ground magnetic Fe after dissolution 3 O 4 The powder is stirred and dispersed evenly and then added to the three-necked flask, and the stirring is continued; 0.1g of potassium persulfate and 0.05g of sodium bisulfite are dissolved in 10ml of water and then added dropwise to the three-necked flask. Deoxidize with nitrogen and react at room temperature for 2h. After the reaction is over, filter out the organic solvent part with a 180-mesh screen, then wash the gel microspheres with acetone, wash repeatedly with water, and soak in 50ml of 1mol / L hydrochloric acid solution. After soaking for sev...

Embodiment 3

[0060] Example 3. Preparation of ovalbumin template surface imprinting gel composite microspheres

[0061] (1) Preparation of bulbs

[0062] Add 120ml of benzene to a 250ml three-necked flask with a stirrer and a vent tube, add 0.25g of methylcellulose, and stir to dissolve it. Add 1g of methacrylic acid to 11.6ml of 1mol / L NaOH solution, stir for 5min, add 8gAM, 1g Bis and 20ml of water, add 0.4g of ground magnetic Fe after dissolution 2 O 3 The powder is stirred and dispersed evenly and then added to a three-necked flask, and stirring is continued; 0.1g of ammonium persulfate and 0.05g of sodium sulfite are dissolved in 10ml of water and then added dropwise to the three-necked flask. Deoxidize with nitrogen and react at room temperature for 2h. After the reaction is over, filter out the organic solvent part with a 180-mesh screen, then wash the gel microspheres with acetone, wash repeatedly with water, and soak in 50ml of 1mol / L hydrochloric acid solution. After soaking for seve...

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Abstract

A composite magnetic gel microsphere for surficial biological macromolecular template blot, based on template blot (molecular blot) technique, is prepared from the different biological macromoleculae as template molecule and different metals (or their oxides) as magnetic component through reverse-phase seed suspension polymerization. Its advantages are less consumption of template moleculae and high adsorption capacity. It can be used for separating biological macromoleculae with more precision and simple process.

Description

Technical field [0001] The invention belongs to the fields of material science and engineering and bioseparation engineering, and more specifically relates to a biomacromolecule template surface imprinted gel magnetic composite microsphere and a preparation method of seed reverse suspension polymerization. Background technique [0002] Molecular recognition and recognition specificity are universal and unique characteristics of the entire biology, and they play a special and important role in biological evolution. Template imprinting is a technology based on the biomimetic of biology, which uses artificial methods to prepare polymers that are specific to specific molecules (template molecules or imprinted molecules). Recently, molecularly imprinted polymers (MIPs) prepared by this technology have shown broad application prospects in separation and purification, immunoassays, mimic enzymes, and biosensors, and become one of the most promising new materials ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D71/00C08F2/12C08F2/44C08K3/22
Inventor 成国祥陆书来张立广庞兴收
Owner TIANJIN UNIV
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