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Biological polysaccharide micro-capsule, prepn. method and application thereof

A biological polysaccharide and microcapsule technology, applied in the field of materials science, can solve the problems of not yet seen biological polysaccharide capsules, unsuitable for vaccine carriers, and too large in size.

Inactive Publication Date: 2006-08-09
上海中科伍佰豪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the excessive size of the aforementioned alginate capsules, they are not suitable for use as intravascular drug carriers nor for use as vaccine carriers
There is no report on the preparation of biopolysaccharide capsules with a diameter of less than 1 micron

Method used

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  • Biological polysaccharide micro-capsule, prepn. method and application thereof
  • Biological polysaccharide micro-capsule, prepn. method and application thereof
  • Biological polysaccharide micro-capsule, prepn. method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of calcium alginate microcapsules

[0038] (1) Take two 50 ml polypropylene centrifuge tubes, each weighing 400 mg of nonionic surfactant Span65, and dissolve in 16.6 ml of dichloromethane respectively. Add 2 ml of sodium alginate solution dissolved in 10 mM HEPES buffer (pH 7.4) and 2 ml of 120 mM CaCl 2 solution (containing 10mM HEPES, pH 7.4), then add mineral oil and mix gently until the oil phase has the same density as the water phase;

[0039] (2) Put the centrifuge tube on the ice bath, and use ultrasonic (Misonic, 1 / 4 inch) to dissolve the sodium alginate solution and CaCl 2 The solutions were emulsified for 4 minutes respectively;

[0040] (3) Sodium alginate emulsion and CaCl 2 Stir the emulsion in a beaker and react for 5 minutes to complete the phase change;

[0041] (4) After the reaction is completed, the reactants are evenly transferred to four 50 ml polypropylene centrifuge tubes, and then dichloromethane is added to 50...

Embodiment 2

[0048] Embodiment 2: the preparation of barium alginate microcapsule

[0049] (1) Take two 50 ml polypropylene centrifuge tubes, each weighing 400 mg of nonionic surfactant Span65, and dissolve in 16.6 ml of dichloromethane respectively. Add 2 ml of sodium alginate solution dissolved in 10 mM HEPES buffer (pH 7.4) and 2 ml of 100 mM BaCl 2 solution (containing 10mM HEPES, pH 7.4), then add mineral oil and mix gently until the oil phase has the same density as the water phase;

[0050] (2) Put the centrifuge tube on ice, and use ultrasonic (Misonic, 1 / 4 inch) to dissolve the sodium alginate solution and BaCl 2 The solutions were emulsified for 4 minutes respectively;

[0051] (3) Sodium alginate emulsion and BaCl 2 Stir the emulsion in a beaker and react for 5 minutes to complete the phase change;

[0052] (4) After the reaction is completed, transfer to four 50ml polypropylene centrifuge tubes on average, then add dichloromethane to 50ml, mix and then centrifuge at 1700RPM...

Embodiment 3

[0059] Embodiment 3: Preparation of ethylenediamine crosslinked alginate microcapsules

[0060] Diamine compounds are used to organically cross-link the alkaline earth metal ion cross-linked alginate microcapsules, and then a chelating agent is used to remove the bound ions.

[0061] (1) 200 milligrams (dry weight) of calcium alginate microcapsules are put into a 50 milliliter polypropylene centrifuge tube, centrifuged, and the supernatant is removed;

[0062] (2) Add 45 ml of 25mM MES (2-N-morpholinoethanesulfonic acid) buffer (containing 20mM CaCl 2 and 108mM NaCl), utilize ultrasound to disperse the capsules, centrifuge, and remove the supernatant;

[0063] (3) Repeat (2);

[0064] (4) Add 62.5 microliters of 1M ethylenediamine (2HCl, pH adjusted to 6.5) dissolved in MES, and mix;

[0065] (5) Weigh 800 mg of EDAC and NHS respectively, quickly dissolve in 5 ml of 20mM MES solution, mix with alginic acid capsules, and mix and react at room temperature for 183 hours;

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Abstract

A biological polyose microcapsule used as the carrier for preparing medicine or vaccine is prepared from the aqueous solution of biologic polyose and the aqueous solution of metallic salt through respectively adding them to the organic solvent containing surfactant, adding mineral oil, stirring to respectively obtain their suspension, respectively ultrasonic emulsifying on ice bath, mixing them together, centrifugal layering to obtain water phase, dewatering and washing.

Description

technical field [0001] The invention relates to the field of materials science, and more specifically relates to biological polysaccharide microcapsules and a preparation method and application thereof. Background technique [0002] With the development of bioengineering research, new drugs will continue to emerge, providing more disease treatments for human health. At the same time, the development of biological drugs and new treatment methods also put forward higher requirements for the development of drug delivery methods. [0003] Common biological polysaccharides include sodium alginate, Gellan gum (gellan gum), pectin, pectic acid, carrageenan, agar and agarose, etc., all of which have a chain structure formed by monosaccharide links. Among them, agar and agarose are thermal sols (Thermal gel), which can melt as the temperature increases and solidify as the temperature decreases; the aqueous solution of sodium alginate, pectin, pectic acid and carrageenan has weak pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K9/50A61K31/732A61K33/14A61K33/06A61K33/24A61K33/26A61K33/30A61K33/34
Inventor 沈秉谦
Owner 上海中科伍佰豪生物工程有限公司