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Fenvalerate direct competition enzyme joint immune absorption analysis technology and its kit

An enzyme-linked immunosorbent assay and fenvalerate technology is applied in the direction of analysis materials, measuring devices, instruments, etc., achieving the effect of simple application method, high sensitivity and advanced technology

Inactive Publication Date: 2007-01-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunoassay method for fenvalerate reported abroad has obvious cross-reaction with some other structural analogues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Example of fenvalerate direct competition enzyme-linked immunosorbent assay method

[0017] Condensed with 2-(4-chlorophenyl)-3-methylbutyryl chloride and amino acid, separated and purified (n=1-5), taking this as hapten, adopting active ester method or mixed acid anhydride method to covalently couple with bovine serum albumin and ovalbumin to synthesize artificial antigen and coating original. Mix and emulsify the artificial antigen with an appropriate amount of Freund's adjuvant to immunize rabbits, sheep, and mice to prepare polyclonal antibodies with specific affinity for fenvalerate or use hybridoma technology to prepare polyclonal antibodies with specific affinity for fenvalerate. Heli Monoclonal Antibody. The horseradish peroxidase was covalently coupled to the antibody by the improved periodate method to prepare the enzyme-labeled antibody, and the hapten was covalently coupled to the horseradish peroxidase by the mixed anhydride method or the active ester ...

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PUM

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Abstract

The invention relates to a analyzing method for fenvalerate direct competition enzyme immunity absorption analysis2 and its kit. Compose hapten (the chemical formula )(n=1-5) and it covalency coupling symphysis with protein to be artificial antigen, use the artificial antigen immunity animal to prepare for antibody with idiosyncratic affinity to fenvalerate and use horseradish peroxide to mark the hapten. Use antibody to surround and cover the plate with microholes, add it into the mixture of the sample (or the marked standard sample) and hapten marked by enzyme , fenvalerate , hapten marked by enzyme and the antibody on the surface of the micro holes have competitive bond , then clear away the educt, add substrate and color-developing agent of enzyme the intensity of enzymatic colour reaction is inversely proportional to the content of fenvalerate in the sample (or the standard sample), in accordance with it, the fenvalerate direct competition enzyme immunity absorption analysis technique is invented. The technology can be applied to choose relative reagent and material in the kit , for preparing kit of immunity test, and rapid test for the fenvalerate remained in agricultural products, tea leaf and environment.

Description

technical field [0001] The invention relates to an analysis method for direct competition of fenvalerate by an enzyme-linked immunosorbent assay and a kit thereof, which are mainly used in the rapid detection of residual fenvalerate in fruits and vegetables, grain, tea and the environment. Background technique [0002] Fenvalerate is a widely used pyrethroid insecticide. It is effective against pests of Lepidoptera, Homoptera, Orthoptera and Hemiptera that harm cotton, fruit trees, vegetables, tea, soybeans, wheat and other crops. (such as cotton bollworm, aphid, cabbage caterpillar, tea caterpillar, soybean borer, etc.) larvae have a good control effect, and can also control cutworms and other noctuid pests. Fenvalerate is moderately toxic to rats and highly toxic to fish and other aquatic organisms. People who take fenvalerate by mistake may cause vomiting, nervousness, palpitation, salivation and other symptoms, and tremor and general convulsions may occur in severe case...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/547G01N33/535G01N33/531
Inventor 刘曙照尤海琴
Owner YANGZHOU UNIV