Process for preparing bioactive peptide-silk fibroin peptide

A silk fibroin peptide and enzymatic hydrolyzate technology, applied in the field of food biology, can solve the problems of serious membrane pollution and difficult to realize industrialized production, and achieve the effects of high desalination rate, large processing capacity and mild separation conditions

Inactive Publication Date: 2003-01-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the desalination process, membrane technologies such as ultrafiltration and electrodialysis are usually used for d...

Method used

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  • Process for preparing bioactive peptide-silk fibroin peptide
  • Process for preparing bioactive peptide-silk fibroin peptide
  • Process for preparing bioactive peptide-silk fibroin peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of silk fibroin peptide powder with degree of hydrolysis (DH)=12%

[0019] Waste silk (nodding) treated with 0.5% (w / v) Na 2 CO 3 After the solution is refined, place it in boiling 40% (w / w) CaCl 2 Dissolve in the solution for 5 minutes and 30 seconds (according to 100ml CaCl 2 The solution dissolves 14 g of scoured silk for dissolving), and cools rapidly. The silk-dissolving solution was filtered through gauze to obtain a concentrated silk-dissolving solution. Take 8 liters of the concentrated silk-dissolving solution, add 12 liters of water, carry out enzymatic reaction with Alcalase enzyme at pH 8.5 and 60°C, and use the pH-stat method to control the degree of hydrolysis. After the reaction is completed, the enzyme is heated to inactivate, centrifuged and filtered, and the filtrate is desalted with a polyamide nanofiltration membrane. During the desalination process, water is continuously added to dilute the feed solution, and the desalted feed solutio...

Embodiment 2

[0038] Preparation of silk fibroin peptide powder with degree of hydrolysis (DH)=10%

[0039] Waste silk (nodding) treated with 0.5% (w / v) Na 2 CO 3 After the solution is refined, place it in boiling 40% (w / w) CaCl 2 Dissolve in the solution for 5 minutes and 30 seconds (according to 100ml CaCl 2 The solution dissolves 14 g of scoured silk for dissolving), and cools rapidly. The silk-dissolving solution was filtered through gauze to obtain a concentrated silk-dissolving solution. Take 7 liters of the concentrated silk-dissolving solution, add 7 liters of water, carry out enzymatic reaction with Alcalase enzyme at pH 8.5 and 60°C, and use the pH-stat method to control the degree of hydrolysis. After the reaction is over, heat to inactivate the enzyme, and after centrifugation and filtration, the filtrate is desalted with a polyethersulfone nanofiltration membrane. During the desalination process, water is continuously added to dilute the feed solution. After desalination, th...

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PUM

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Abstract

The present invention, the preparation process of silk fibroin peptide as one bioactive peptide, belongs to the field of food biotechnology. Silkworm cocoon leftover or waste cocoon as material is refined in Na2CO3 solution and dissolved in CaCl2 solution to obtain silk dissolving liquid; the silk dissolving liquid is enzyme hydrolyzed at pH 2.5-12.0 and 30-80 deg.c, nano filtered to elimiante high-concentration CaCl2 salt, concentrated and spray dried to obtain the product silk fibroin peptide powder. The present invention has the advantages of great treated amount, high desalting rate, mildseparation condition, simple process and low power consumption. The said process may be used in industry to prepare silk fibroin peptide for functional food.

Description

technical field [0001] The present invention relates to a preparation method of biologically active peptide-silk fibroin peptide, in particular to a method for preparing silk fibroin peptide by hydrolyzing silk solution by enzymatic method, and then removing high-concentration calcium chloride in the enzymatic hydrolysis solution by nanofiltration technology, It belongs to the field of food biotechnology. Background technique [0002] Silk is a kind of fibrin, composed of silk fibroin and sericin, of which silk fibroin accounts for 70% to 80%, sericin accounts for 20% to 30%, silk fibroin is composed of 18 kinds of amino acids, and about 85% are glycine, alanine amino acid, serine and tyrosine. Silk peptide (silk peptide) is an intermediate product of silk fibroin hydrolysis. Usually, the degummed silk fibroin is dissolved, dialyzed, purified, degraded, concentrated and other processes, and then spray-dried to produce high-purity silk peptide powder. Silk peptide has the s...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 王璋杨瑞金许时婴周凤娟倪莉
Owner JIANGNAN UNIV
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