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Deel-layer fermentation process for culturing Brazilian cordyceps mycelia

A technology of submerged fermentation culture and Cordyceps mycelium, applied in the direction of fungi, etc., can solve the problems of limited natural Cordyceps sources and high prices

Inactive Publication Date: 2003-02-19
广州市微生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the source of natural Cordyceps is limited and expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Shake flask culture

[0017] For a 50-liter seed tank, the inoculation amount is 3% based on the filling volume of 30 liters, and the volume of the shake flask medium is 30 liters×3%=0.9 liters. According to starch 1%, glucose 1%, pupa casein 1%, MgSO 4 0.05%, K 2 HPO 4 The formula of 0.05%, pH 6.8, with 0.9 liters of culture solution, is divided into 500 ml conical flasks, each bottle is 100 ml, sterilized after filling, cooled to 30 ℃, and inserted into test tubes. Each bottle is connected to 2 seed blocks about 0.5 square centimeters. The rotating speed of the shaker was 180 rpm, and the temperature was controlled at 25°C.

[0018] (2) Seed tank cultivation

[0019] The actual filling volume of a 50-liter seed tank is 30 liters, minus 0.9 liters of the shake bottle seed volume, it is 30-0.9=29.1 liters. According to the formula described in this example (1), with 29.1 liters of culture medium (actually only add water to 26 liters, it is expected that ther...

Embodiment 2

[0022] Example 2 Comparison of main components between Cordyceps mycelium powder and natural Cordyceps

[0023] Table 1, Table 2, and Table 3 are the comparisons of the main active ingredients, trace elements, and amino acid contents of Cordyceps mycelium powder and natural Cordyceps.

[0024] project

[0025] element

[0026] amino acid

[0027] From the results of Tables 1, 2 and 3 above, as the main active ingredients of Cordyceps sinensis D-mannitol (cordycepic acid), ergosterol (cordycepin), and crude polysaccharide, the content of cordyceps mycelium powder is slightly higher than that of natural cordyceps sinensis. The amino acid content of the two is basically the same, and the trace element content is not much different, indicating that the chemical composition of the two is basically the same, laying the foundation for the Cordyceps mycelium powder to replace the natural Cordyceps.

Embodiment 3

[0028] Example 3 Comparison of pharmacological effects between Cordyceps mycelium powder and natural Cordyceps

[0029] (1) Effects on the weight of immune organs and white blood cells

[0030] 80 NIH mice with a body weight of 12±2g were taken and divided into 8 groups, 10 mice in each group. They were given different doses by intragastric administration, qd×7d, with a volume of 0.2ml / 10g. The control group was given equal volume of distilled water. Immunocompromised groups 5-8 were given intraperitoneal injection of cyclophosphamide 50 mg / kg on the 4th day after administration. One hour after the last administration, orbital blood was collected to count white blood cells, and the animals were sacrificed by cervical dislocation. The thymus was dissected, and the spleen was weighed with a torsion balance. Wet weight, calculated immune organ index (mg / 10g body weight), the results are shown in Table 4.

[0031] group

[0032] Compared with distilled wate...

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PUM

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Abstract

A deep fermentation and culture method for Brazilian cordyceps mycelia features that the Brazilian cordyceps seed is used as initial fungus, a new culture medium is used, and the cordyceps mycelia are cultured at proper temp. stirring speed and basicity and then dried for preparing high-purity cordyceps mycelia powder. Its advantage is large production scale.

Description

technical field [0001] The invention belongs to the field of biological fermentation, and more specifically relates to a method for submerged fermentation and culture of Cordyceps braziliani mycelium. Background technique [0002] Cordyceps Sinesis (Berk) Sacc is a valuable tonic and strong medicine, it has a wide range of medicinal values, such as enhancing cellular immunity and humoral immunity, androgen-like effects. However, the source of natural Cordyceps is limited and expensive. SUMMARY OF THE INVENTION [0003] The invention provides a method for producing Cordyceps mycelium powder by submerged fermentation with Cordyceps brasiliensis strains. [0004] The object of the present invention is to be solved by the following technical solutions: [0005] Using Cordyceps brasiliensis strains, the newly developed medium formula is used to aerate and cultivate in a fermenter, and the appropriate temperature, stirring speed, pH and other culture conditions are controlled ...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 梁淑娃彭中健杜少平杨冠东
Owner 广州市微生物研究所
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