Immobile bacillus and method for resolving and preparing chiral cyclopentenylone using said bacillus

Abstract

The present invention discloses a kind of immobile bacillus CGMCC0789 and the preparation process of chiral cyclopentenylone with the bacillus. During the preparation, microbe cell with esterase activity or its separated esterase is used as catalyst and fatty ester racemic alcohol ketone is used as material to produce conversion reaction to obtain the mixture of (R)-alcohol ketone fatty ester and(S)-alcohol ketone fatty ester. The mixture is then made to produce sulfonylation and alkali hydrolysis and the final product single (S)-alcohol ketone natipode is collected from the hydrolysate. Thepresent invention has simple technological process, mid reaction condition, bacteria strain with high catalytic activity and stereo selectivity, and the product (S)-alcohol ketone has optical purity over 90%.

Description

technical field

[0001] The invention belongs to the field of biochemical engineering, and relates to an Acinetobacter and a method for preparing enantiomers of chiral compounds by using the microorganism to catalyze the resolution, in particular to a method for preparing cyclopentenone-type chiral alcohol enantiomers by resolution . Background technique

[0002] The cyclopentenone-type chiral alcohol compound is an important intermediate for producing pyrethroid insecticides. The basic skeleton of the cyclopentenone-type alcohol compound involved in the present invention is: 4-hydroxyl-3-methyl- Cyclopent-2-en-1-one mainly includes two varieties, the chemical structural formula is as follows:

[0003] Wherein: formula (I) is 2-allyl-3-methyl-4-hydroxycyclopent-2-en-1-one, hereinafter referred to as allyl alcohol ketone; formula (II) is 2-propargyl- 3-methyl-4-hydroxycyclopent-2-en-1-one, hereinafter referred to as propargyl alcohol ketone; the two can be collectively ref...