Assembled type compound array chip and its preparing method

Abstract

An assembled compound array chip is composed of supporter, several carriers assembled on said supporter, and polymer moleculae (oligonucleotide, polypeptide, polyose and peptide nucleic acid) synthesized at known positions on relative carrier. After the polymer moleculae act on particular fluid, the change of its natural can be detected optically.

Description

technical field [0001] The assembled compound array chip and its preparation method of the present invention relate to an assembled compound molecular detection chip and its preparation process. Background technique [0002] Combinatorial chemistry is a fast way to obtain a large number of different compounds. For macromolecular compounds such as oligonucleotides and polypeptides, which are composed of a small number of small molecular monomers, a large number of different compounds of the same type can be obtained in a relatively short period of time by combinatorial chemistry methods. These different compounds can be constructed into array biochips by means of assembly. Biochips mainly refer to microarrays composed of biologically active substances (including nucleic acids, proteins, cells, and microtissues) assembled on the surface of solid-phase carriers to realize the detection of compounds (including drugs), proteins, nucleic acids, cells, and other biological compone...

AI Technical Summary

Problems solved by technology

The main disadvantage of in situ synthesis is that the contamination of coupling reagents during the synthesis process leads to errors in the synthesis of products, and it is difficult to ensure that the reaction conditions of all sites are completely consistent during the coupling process, resulting in the compound molecules on the synthesis sites. There is a difference between quality and quantity, and the final information error rate is high (if the same oligonucleotide sequence is synthesized, the results given by hybridization will be different)

In addition, although in-situ synthesis has low cost in mass production, the existing in-situ synthesis methods have the disadvantage of poor flexibility, and it takes a lot of manpower and financial resources to prepare a set of mask plates (masks) to prepare any kind of chip. The number of plates is generally four times the number of bases in the synthetic oligonucleotide) design and production; the preparation of low-density chips by the spotting method is to first synthesize and purify the oligonucleotide sequence, and then synthesize it in the oligonucleotide sequence The active groups on the substrate are connected to the active groups on the corresponding positions on the substrate

Its main disadvantage is the high cost of purchasing probes, and the price of synthesizing an active group (such as amino group, etc.) fixed on the substrate on the probe is currently equivalent to the price of synthesizing a 50-base oligonucleotide sequence

Secondly, the difference in sample volume and reaction conditions during the sample application process will also lead to inconsistencies in the number of molecules of the target compound at each position.

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