Multiprobe optical fibre evanescent wave biological sensor

Abstract

The invention is a multiple-probe fibre-optical evanescent-wave biosensor, including the laser exciting light path, the fluoescent receiving light path, the sample flow path and the scan system, respectively used to excite the fluorescence of the measured biological substance on the surface of the fibre-optical core wire, receive the fluorescent signal from the fibre-optical and finish the photoelectric conversion, indraft the sample and make the scanning detection on the fluorescent signal on multiple pieces of fiber-optical.

Description

Technical field: [0001] The invention relates to a multi-probe optical fiber evanescent wave biosensor, which is widely used in the fields of biomedicine, food inspection, environmental monitoring, etc. It can detect hormones, pollutants, toxic substances, explosives, deoxyribonucleic acid (DNA ), viruses, various bacteria (such as anthrax, Escherichia coli O157, etc.) and other biological substances, and can also monitor the dynamic process of biological reactions. Background technique: [0002] The multi-probe optical fiber evanescent wave biosensor uses the evanescent wave generated when the light wave is transmitted in the optical fiber by total reflection to excite biomolecules labeled with fluorescent dyes that attach to the surface of the optical fiber core wire through a biological affinity reaction. Put the optical fiber with biorecognition molecules immobilized on the surface into the sample. If the biomolecules labeled with fluorescent dyes in the sample come from...

AI Technical Summary

Problems solved by technology

[0005] 1. Low fluorescence excitation efficiency

The laser beam output by the laser light source 1 enters the optical fiber 5 after being focused by the coupling lens 4, the numerical aperture of the laser beam entering the optical fiber 5 is small, and the reflection times of the light beam in the optical fiber 5 are few; moreover, the incident angle range of the laser beam entering the optical fiber 5 It starts from 0°, because the smaller the angle of the light, the greater the intensity, and the lower the excitation efficiency of the light with the smaller angle

At the same time, the small-angle light has fewer reflections in the fiber, and the penetration depth is small, and the excitation range is small

[0006] 2. Low utilization rate of light energy

So the utilization rate of the laser beam is only 50%

[0007] 3. Low fluorescence reception efficiency

[0008] The above three reasons lead to the low detection sensitivity of the prior art, only 1.4×10 -8 mole / liter

[0009] 4. Low detection efficiency

Images