Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Longbract cattail general flavone extractive and its prepn and use

A technology of extracts and total flavonoids, applied in the field of medicine, can solve problems such as the undiscovered total flavonoids extract from Puhuang

Inactive Publication Date: 2004-01-21
FUDAN UNIV
View PDF1 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no patents or publications related to the total flavonoids extract of Puhuang have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Put 1kg of raw Pollen in a 10L round bottom flask, add 7kg of 70% ethanol, stir well, heat and reflux in a water bath for extraction for 2 hours, filter while hot, and then heat and extract the residue with 5kg of 70% ethanol for 1 hour , filtered while hot, and combined the filtrates. The filtrate is reclaimed under reduced pressure with a rotary evaporator to a thick extract with a specific gravity of 1.1 to 1.2, and 10% ethanol solution is added in a ratio of 1:10, and a chitosan clarifying agent is added in a ratio of 1% of the total volume simultaneously, and the Centrifuge and filter after reaching room temperature. The filtrate is adsorbed on the pre-treated 1kg HZ-802 macroporous adsorption resin (produced by Shanghai Huazhen Technology Trading Co., Ltd.) column bed. After all the filtrate passes through, the column bed is first washed with 8 to 10 liters of deionized water until clarified. , and then use 10 to 12 liters of 30% ethanol to rinse until the color ...

Embodiment 2

[0046] Put 1kg of raw Pollen in a 10L round bottom flask, add 7kg of 70% ethanol, stir well, heat and reflux in a water bath for extraction for 2 hours, filter while hot, and then heat and extract the residue with 5kg of 70% ethanol for 1 hour , filtered while hot, and combined the filtrates. The filtrate was recovered to 3 L (the specific gravity was about 1.05) with a rotary evaporator under reduced pressure, and 500 ml of saturated lead acetate aqueous solution was added, fully stirred, and allowed to stand for precipitation. The precipitate was collected by centrifugation. Suspend the precipitate in 2.5L of 95% ethanol, pass through H2S for metathesis, centrifuge to remove the lead sulfide precipitate, recover ethanol from the filtrate, vacuum-dry and pulverize to obtain 143 g of total flavonoids extract of Puhuang. After determination, the total flavonoid content is 54.2%. Embodiment 3: the preparation technology of high-purity total flavonoids of Puhuang

Embodiment 3

[0047] Get 5g of the total flavonoids extract of Puhuang obtained in the above-mentioned embodiment 1 or embodiment 2, dissolve in an appropriate amount of methanol, 500g Sephadex-LH20 column chromatography on the solution, elute with methanol, collect the main color bands respectively, reclaim the solvent to After drying, 1.25 g of isorhamnetin-3-O-(2G-α-L-rhamnosyl)-rutinoside and 0.98 g of isorhamnetin-3-O-neohesperidoside were obtained respectively. After determination, the content of the former component is 96.4%; the content of the latter component is 98.5%. Embodiment 4: the preparation technology of sodium salt derivative

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the active general flavone components extracted from longbract cattail as one kind of Chinese medicinal materials. The extractive includes mainly isorhamnetin, quercetin, kaempferol and their glycoside derivatives, and also includes the degraded products of the said components, their metal salt derivatives, metal complexes, etc. The extractive may be obtained via solvent extraction process and other process. The extractive has the functions of lowering blood fat, resisting atherosclerosis, raising the tolerance of cardiac and cerebral tissues to deficiency of oxygen and blood, resisting platelet aggregation, etc. and may be used in preventing and treating cardiac and cerebral vascular diseases, traumatic injury, haemorrhages and other diseases.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a total flavonoid extract of the traditional Chinese medicine Puhuang, its preparation process and its application in medicine and health food. Background technique [0002] Cattail is a commonly used traditional Chinese medicine, derived from Typha angustifolia L., Typha angustifolia L., T. arientalis Presl., or the dried pollen of other plants belonging to the family Typhaceae. The theory of traditional Chinese medicine believes that Puhuang has the functions of stopping bleeding, removing blood stasis, and dredging stranguria. disease. Modern pharmacological studies have shown that water or alcohol extracts of Puhuang can significantly increase coronary flow, improve microcirculation, increase the tolerance of myocardium and brain to hypoxia, or reduce the oxygen consumption of heart and brain tissue, dilate blood vessels, lower blood lipids, Anti-atheroscleros...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/7048A61K36/88A61P7/04A61P9/00C07D311/30C07D311/40
CPCC07D311/40C07D311/30A61K31/7048A61K31/352A61K36/88A61P7/04A61P9/00A61P29/00A61K2300/00
Inventor 周铜水
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com