Method for breeding corn with low phytic acid content

A phytic acid and corn technology, applied in the breeding field of corn with low phytic acid content, can solve the problems of reduced bioavailability, high cost, environmental pollution, etc., to reduce the problem of phosphorus enrichment, improve utilization rate, and reduce production cost effect

Inactive Publication Date: 2004-09-15
ZHEJIANG UNIV
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Problems solved by technology

Ingested phytic acid can further combine with other sources of micromineral nutrients to form phytate, resulting in a decrease in the bioavailability of these nutrients, thereby exacerbating micronutrient deficiencies
Although adding phytase to the feed can play a similar role, there are many restrictions on the feed processing technology, and the highest decomposition rate can only reach 80%, and the cost is high
In addition, 65-80% of phosphorus (P) in corn grai

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Maize 1pa-HC2 with low phytic acid content was selected by physical mutagenesis of maize variety Yellow C:

[0020] The immature embryos of maize variety Huang C were taken to induce the formation of callus, and treated with cobalt-60 γ-ray mutagenesis at a dose of 5Gy. The above-mentioned calli were differentiated into seedlings, the mutagenized contemporary plants were planted, artificially pollinated, selfed, bagged and isolated, and the seeds of all the mutagenized plants of the first generation were harvested together. 15,000 plants of the second-generation population were mixed for mutagenesis, artificially pollinated, self-crossed and isolated in bags, and the seeds of the third-generation mutagenesis were harvested per plant, threshed and numbered per plant. Randomly select 8 mature seeds and take single grain as a unit, adopt the method of routine determination of inorganic phosphorus content to indirectly screen for low phytic acid mutations, select and measur...

Embodiment 2

[0022] Breeding corn 1pa-X178 with low phytic acid content by chemical mutagen ethyl methanesulfonate corn variety X178:

[0023] Take 3000 dry seeds of maize variety X178, and use the chemical mutagen ethyl methanesulfonate to induce mutagenesis at room temperature (20-25° C.), and the treatment concentration is 2 Mm / L·h. The seeds of the mutagenized generation were planted, artificially pollinated, self-crossed and isolated in bags, and the seeds of all the mutagenized plants of the first generation were mixed. 6,000 plants of the second-generation population were mixed for mutagenesis, artificially pollinated, self-crossed and isolated in bags, and the third-generation seeds were mixed for mutagenesis. 35,000 plants of the third-generation population were mixed and planted, artificially pollinated, self-crossed and isolated in bags. Randomly select 8 mature seeds and take single grain as the unit, adopt the conventional method of measuring inorganic phosphorus content to i...

Embodiment 3

[0025] Low-phytic-acid-content corn 1pa-HC2 was used as a parent to backcross and breed low-phytic-acid-content corn:

[0026] The low phytic acid content maize 1pa-HC2 was used as the male parent, and it was crossed with the common maize variety Huangyesi to plant the first-generation hybrids, artificially pollinated, self-crossed and isolated by bagging, and mixed the second-generation hybrid seeds of all the first-generation hybrid plants. 5,000 second-generation hybrids were planted in a mixed population, artificially pollinated, self-crossed and isolated in bags, and the third-generation hybrid seeds were harvested and threshed per plant. Randomly select 8 mature hybrid seeds of the third generation, and take a single seed as a unit, adopt the method of measuring inorganic phosphorus content to indirectly screen materials with low phytic acid content, select and measure all or a few seeds that are dyed blue in the seed extract, and eliminate the seeds for extraction Seeds...

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Abstract

The present invention relates to a breed selection method of low phytic acid content corn, said method uses conventional corn variety or parent of hybrid species as test material and adopts the following steps: using mutagenic agent to make mutagenization, in mutagenic second generation or mutagenic third generation adopting the method for determing inorganic phosphorus content to indirectly screen low phytic acid mutant, and in mutagenic third generation or fourth generation evaluating stability and isozygoty of low phytic acid mutant expression, on this basis, evaluating genetic control, agronomical character and combining ability of different low phytic acid mutants so as to breed out low phytic acid content corn, and using it as raw material to produce green feed.

Description

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Claims

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Application Information

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Owner ZHEJIANG UNIV
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