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Method for breeding corn with low phytic acid content

A phytic acid and corn technology, applied in the breeding field of corn with low phytic acid content, can solve the problems of reduced bioavailability, high cost, environmental pollution, etc., to reduce the problem of phosphorus enrichment, improve utilization rate, and reduce production cost effect

Inactive Publication Date: 2004-09-15
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

Ingested phytic acid can further combine with other sources of micromineral nutrients to form phytate, resulting in a decrease in the bioavailability of these nutrients, thereby exacerbating micronutrient deficiencies
Although adding phytase to the feed can play a similar role, there are many restrictions on the feed processing technology, and the highest decomposition rate can only reach 80%, and the cost is high
In addition, 65-80% of phosphorus (P) in corn grains exists in the form of phytate phosphorus. These phytate phosphorus and other phytic acid (salt) cannot be absorbed and utilized by humans and monogastric animals, and are excreted with feces, causing serious environmental pollution problems , especially water eutrophication; due to the lack of decomposing microorganisms in the soil, even if livestock and poultry manure is returned to the field as organic fertilizer, it cannot be absorbed and utilized by crops

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Maize 1pa-HC2 with low phytic acid content was selected by physical mutagenesis of maize variety Yellow C:

[0020] The immature embryos of maize variety Huang C were taken to induce the formation of callus, and treated with cobalt-60 γ-ray mutagenesis at a dose of 5Gy. The above-mentioned calli were differentiated into seedlings, the mutagenized contemporary plants were planted, artificially pollinated, selfed, bagged and isolated, and the seeds of all the mutagenized plants of the first generation were harvested together. 15,000 plants of the second-generation population were mixed for mutagenesis, artificially pollinated, self-crossed and isolated in bags, and the seeds of the third-generation mutagenesis were harvested per plant, threshed and numbered per plant. Randomly select 8 mature seeds and take single grain as a unit, adopt the method of routine determination of inorganic phosphorus content to indirectly screen for low phytic acid mutations, select and measur...

Embodiment 2

[0022] Breeding corn 1pa-X178 with low phytic acid content by chemical mutagen ethyl methanesulfonate corn variety X178:

[0023] Take 3000 dry seeds of maize variety X178, and use the chemical mutagen ethyl methanesulfonate to induce mutagenesis at room temperature (20-25° C.), and the treatment concentration is 2 Mm / L·h. The seeds of the mutagenized generation were planted, artificially pollinated, self-crossed and isolated in bags, and the seeds of all the mutagenized plants of the first generation were mixed. 6,000 plants of the second-generation population were mixed for mutagenesis, artificially pollinated, self-crossed and isolated in bags, and the third-generation seeds were mixed for mutagenesis. 35,000 plants of the third-generation population were mixed and planted, artificially pollinated, self-crossed and isolated in bags. Randomly select 8 mature seeds and take single grain as the unit, adopt the conventional method of measuring inorganic phosphorus content to i...

Embodiment 3

[0025] Low-phytic-acid-content corn 1pa-HC2 was used as a parent to backcross and breed low-phytic-acid-content corn:

[0026] The low phytic acid content maize 1pa-HC2 was used as the male parent, and it was crossed with the common maize variety Huangyesi to plant the first-generation hybrids, artificially pollinated, self-crossed and isolated by bagging, and mixed the second-generation hybrid seeds of all the first-generation hybrid plants. 5,000 second-generation hybrids were planted in a mixed population, artificially pollinated, self-crossed and isolated in bags, and the third-generation hybrid seeds were harvested and threshed per plant. Randomly select 8 mature hybrid seeds of the third generation, and take a single seed as a unit, adopt the method of measuring inorganic phosphorus content to indirectly screen materials with low phytic acid content, select and measure all or a few seeds that are dyed blue in the seed extract, and eliminate the seeds for extraction Seeds...

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Abstract

The present invention relates to a breed selection method of low phytic acid content corn, said method uses conventional corn variety or parent of hybrid species as test material and adopts the following steps: using mutagenic agent to make mutagenization, in mutagenic second generation or mutagenic third generation adopting the method for determing inorganic phosphorus content to indirectly screen low phytic acid mutant, and in mutagenic third generation or fourth generation evaluating stability and isozygoty of low phytic acid mutant expression, on this basis, evaluating genetic control, agronomical character and combining ability of different low phytic acid mutants so as to breed out low phytic acid content corn, and using it as raw material to produce green feed.

Description

technical field [0001] The invention relates to a method for breeding corn with low phytic acid content. Background technique [0002] Corn is commonly known as "the king of feed". 75% of the total corn production is used for feed processing, accounting for about 60% of the total feed raw materials. Therefore, the high quality of corn for feed use and the research, development and utilization of "feed special corn" have great social and economic significance, and have a dual impact on improving the international competitiveness of my country's agriculture. For animals, many nutrients in corn exist in an unavailable state, and there are many anti-nutritional factors, which reduce the effective nutritional level of corn and cause environmental pollution. Phytates such as phytate phosphorus and calcium phytate are typical examples. Therefore, increasing the proportion of available nutrients and reducing the content of anti-nutritional factors is a b...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 吴殿星舒庆尧
Owner ZHEJIANG UNIV
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