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Method for culturing nostoc using bioreactor

A bioreactor, the technology of glutinous rice, which is applied in the biological field, can solve the problems such as the decline of the output of glutinous rice, the destruction of natural ecology and the reduction of product quality, and achieve the effects of good product quality, short growth process and convenient operation.

Inactive Publication Date: 2004-09-15
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

So contradictions emerged. On the one hand, the output of kudzu rice decreased, and the product quality gradually decreased; while people's demand increased, on the other hand, the production area decreased, and a large number of harvests destroyed the natural ecology, making the already poor kudzu rice. The production of sage rice continues to decline

Method used

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Embodiment Construction

[0022] Utilize bioreactor to cultivate the concrete implementation steps of method for kudzu gexianmi as follows:

[0023] 1. Culture solution preparation: The order of adding the weighed or prepared storage solution into water is: add 0.075g of magnesium sulfate, 0.036g of calcium chloride, 0.006g of citric acid, 0.006g of ammonium citrate, and ethylene diacetate per liter of water. Disodium tetraacetate (EDTA) 0.001g, sodium carbonate 0.03g, trace element A51ml, dipotassium hydrogen phosphate 0.07g.

[0024] 2. Preparation of Gexianmi seeds:

[0025] 2.1 First-level seed preparation: connect the original seed of the test tube to a small Erlenmeyer flask (150ml), place it on a culture rack (or box), and place it under a light of 10μE.m -2 .s -1 , static culture or aerated culture at a temperature of 28°C; cultivate until fine particles appear, then transfer to secondary seed culture.

[0026] 2.2 Secondary seed preparation: connect the primary seeds to a 500-1000ml culture...

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Abstract

The present invention discloses a method for culturing nostoc paludosum by utilizing biological reactor. Said method includes the following steps: (1) preparing culture liquor, adopting magnesium sulfate, calcium chloride, citric acid, ammonium ferric citrate, disodium oxalate tetracetate, sodium carbonate, trace element liquor A5 and dipotassium hydrogen phosphate to make preparation according to a certain proportion; (2). algae seed preparation, using the culture of spherical body obtained from second-grade or third-grade culture as inoculative seed source; (3). using biological reactor to make culture, controlling temp., light intensity and ventilation condition of culture liquor; (4). preparing inoculum; (5). culture; (6) monitoring; (7) harvesting; (8) raising mechanical strength of nostoc paludosum spherical body; and (9) drying.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a method for producing and cultivating Gexianmi by using a bioreactor. Background technique [0002] Gexianmi is a kind of cyanobacteria, formerly known as "Tianxianmi", scientific name Nostoc sphaeroides, is a species of Cyanophyceae, Nostocaceae, Nostoc genus. Gexianmi is a treasure on the table, a treasure in diet therapy, and a treasure in medicinal diet. It is even called the delicacy of the world, and it is a rare delicacy. The written records of Chinese people eating spirulina are the oldest in the world, and the understanding of Gexianmi has been known for 2,000 years. According to the world famous book "Compendium of Materia Medica": "Ge Xianmi, born on the rocks of the caves along the streams and mountains in Huguang, when heavy rains washed the caves, the rice came out with the flow. The natives scooped it up, and when they were fi...

Claims

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Application Information

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IPC IPC(8): A01H13/00
Inventor 沈银武刘永定吴国樵李敦海刘其芳丘昌强
Owner INST OF AQUATIC LIFE ACAD SINICA
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