Agglutinin II protein of rhizome of king solomonseal, and application

A technology of lectin and Polygonatum, which is applied in the field of Polygonatum lectin II protein, achieves the effects of simplifying the process, expanding the scope of application, and being suitable for large-scale

Inactive Publication Date: 2005-01-12
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are dozens of anti-HSV drugs studied at home and abroad, but only acyclovir (ACV) is more effective in

Method used

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  • Agglutinin II protein of rhizome of king solomonseal, and application
  • Agglutinin II protein of rhizome of king solomonseal, and application
  • Agglutinin II protein of rhizome of king solomonseal, and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Preparation of Polygonatum Lectin II Protein

[0016] In this embodiment, the preparation method of the luteinum lectin II protein is as follows:

[0017] 1 Crude preparation

[0018] Extract sliced ​​Polygonatum tuber with normal saline solution (G / V=1:5) overnight, mash it into a paste with a high-speed tissue grinder, soak it with normal saline solution overnight, filter with gauze, and centrifuge (4°C) , 4500r / min, 30min), add solid ammonium sulfate to the supernatant to reach 30% saturation, place at 4°C overnight, centrifuge (4°C, 4500r / min, 30min) to remove precipitation, continue to add ammonium sulfate to the supernatant to 90%, The precipitate was collected by centrifugation, dissolved in distilled water, dialyzed to remove salt, and freeze-dried to obtain a crude sealogen lectin.

[0019] 2 Affinity column separation and purification

[0020] Take 200 mg of the crude product and dissolve it in 20 ml of normal saline solution, dialyze to balan...

Embodiment 2

[0023] Example 2: Inhibition of Polygonatum Lectin II Protein on Human Herpes Simplex Virus

[0024] 1 Materials and methods

[0025] 1.1 Materials and Instruments

[0026] Polygonatum lectin II protein: prepared according to the method described in Example 1.

[0027] Cells: African green monkey kidney cells (Vero cell line), cell lines provided by the School of Basic Medicine, West China Medical Department, Sichuan University. Virus: Herpes simplex virus type II (HSV-II), 333 strains, stored at -70°C, provided by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0028] Culture medium: RPMI-1640 (GIBCO product) was dissolved according to the instructions, adjusted to pH 7.2, sterilized by microporous membrane filtration, and calf serum (Chengdu Harry Biological Engineering Co., Ltd.) was added to a concentration of 10%, containing 100U / ml Penicillin (Chongqing Yaoyou Pharmaceutical Co., Ltd.) and 100 μg / ml streptomycin sulfate (Shanghai Siyao Co., Ltd.).

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Abstract

A polygonalectin II protein is prepared from siberian solomonseal rhizome through preparing coarse product, affinity column separating and purifying byuse of mannose-Sepharose 4B as absorbent, purifying by molecular sieve Sephacyl S-100, eluting with physiologic saline, collecting, dialyzing for removing salt, and freeze drying. It can suppress the activity of HSV, so it can be used to treat herpes simplex.

Description

technical field [0001] The invention belongs to plant lectins and applications thereof, in particular to Polygonatum cyrtonema Hua., a Chinese herbal medicine of the Liliaceae plant, Polygonatum cyrtonema Hua. Background technique [0002] Lectin is a kind of non-enzyme, non-immune-derived protein or glycoprotein that has sugar-binding specificity and can precipitate sugar complexes widely in nature. Since the first plant lectin was found in castor bean, hundreds of lectins have been discovered and isolated. [0003] Polygonatum cyrtonema Hua., a plant of the Liliaceae family, is a traditional Chinese herbal medicine. Bao Jinku et al. published a paper on "Journal of Biochemistry" (Volume 12, No. 2, April 1996) "Purification of Polygonatum cyrtonema Hua. Part of the property research "introduced a Polygonatum lectin II, this Polygonatum lectin II is an acidic glycoprotein, using the plant Polygonatum tuber as raw material, the preparation ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P31/22
Inventor 吴传芳常丽青荣艳珍高顺赵曦陈放鲍锦库
Owner SICHUAN UNIV
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