Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Agglutinin II protein of rhizome of king solomonseal, and application

A technology of lectin and Polygonatum, which is applied in the field of Polygonatum lectin II protein, achieves the effects of simplifying the process, expanding the scope of application, and being suitable for large-scale

Inactive Publication Date: 2005-01-12
SICHUAN UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are dozens of anti-HSV drugs studied at home and abroad, but only acyclovir (ACV) is more effective in the clinical treatment of HSV infection, and many drug-resistant strains have been found to occur. HSV drug imperative

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Agglutinin II protein of rhizome of king solomonseal, and application
  • Agglutinin II protein of rhizome of king solomonseal, and application
  • Agglutinin II protein of rhizome of king solomonseal, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Preparation of Polygonatum Lectin II Protein

[0016] In this embodiment, the preparation method of the luteinum lectin II protein is as follows:

[0017] 1 Crude preparation

[0018] Extract sliced ​​Polygonatum tuber with normal saline solution (G / V=1:5) overnight, mash it into a paste with a high-speed tissue grinder, soak it with normal saline solution overnight, filter with gauze, and centrifuge (4°C) , 4500r / min, 30min), add solid ammonium sulfate to the supernatant to reach 30% saturation, place at 4°C overnight, centrifuge (4°C, 4500r / min, 30min) to remove precipitation, continue to add ammonium sulfate to the supernatant to 90%, The precipitate was collected by centrifugation, dissolved in distilled water, dialyzed to remove salt, and freeze-dried to obtain a crude sealogen lectin.

[0019] 2 Affinity column separation and purification

[0020] Take 200 mg of the crude product and dissolve it in 20 ml of normal saline solution, dialyze to balan...

Embodiment 2

[0023] Example 2: Inhibition of Polygonatum Lectin II Protein on Human Herpes Simplex Virus

[0024] 1 Materials and methods

[0025] 1.1 Materials and Instruments

[0026] Polygonatum lectin II protein: prepared according to the method described in Example 1.

[0027] Cells: African green monkey kidney cells (Vero cell line), cell lines provided by the School of Basic Medicine, West China Medical Department, Sichuan University. Virus: Herpes simplex virus type II (HSV-II), 333 strains, stored at -70°C, provided by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0028] Culture medium: RPMI-1640 (GIBCO product) was dissolved according to the instructions, adjusted to pH 7.2, sterilized by microporous membrane filtration, and calf serum (Chengdu Harry Biological Engineering Co., Ltd.) was added to a concentration of 10%, containing 100U / ml Penicillin (Chongqing Yaoyou Pharmaceutical Co., Ltd.) and 100 μg / ml streptomycin sulfate (Shanghai Siyao Co., Ltd.).

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

A polygonalectin II protein is prepared from siberian solomonseal rhizome through preparing coarse product, affinity column separating and purifying byuse of mannose-Sepharose 4B as absorbent, purifying by molecular sieve Sephacyl S-100, eluting with physiologic saline, collecting, dialyzing for removing salt, and freeze drying. It can suppress the activity of HSV, so it can be used to treat herpes simplex.

Description

technical field [0001] The invention belongs to plant lectins and applications thereof, in particular to Polygonatum cyrtonema Hua., a Chinese herbal medicine of the Liliaceae plant, Polygonatum cyrtonema Hua. Background technique [0002] Lectin is a kind of non-enzyme, non-immune-derived protein or glycoprotein that has sugar-binding specificity and can precipitate sugar complexes widely in nature. Since the first plant lectin was found in castor bean, hundreds of lectins have been discovered and isolated. [0003] Polygonatum cyrtonema Hua., a plant of the Liliaceae family, is a traditional Chinese herbal medicine. Bao Jinku et al. published a paper on "Journal of Biochemistry" (Volume 12, No. 2, April 1996) "Purification of Polygonatum cyrtonema Hua. Part of the property research "introduced a Polygonatum lectin II, this Polygonatum lectin II is an acidic glycoprotein, using the plant Polygonatum tuber as raw material, the preparation ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/16A61P31/22
Inventor 吴传芳常丽青荣艳珍高顺赵曦陈放鲍锦库
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com