Method for producing super oxide mutase complex enzyne at large scale using grain seed

A technology of superoxide and dismutase, which is applied in oxidoreductase, ultrafiltration, pharmaceutical formulations, etc., can solve the problems of expensive medicine, environmental pollution, and high cost, and achieve good controllable effect of enzyme activity, low energy consumption, low cost effect

Inactive Publication Date: 2005-03-02
陈欣
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  • Summary
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AI Technical Summary

Problems solved by technology

Some processes need to use a large amount of ammonium sulfate to extract, causing environmental pollution
The medicaments used in some crafts are expensive, the cost is too high, and even poisonous, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment one, processing takes corn grain as basic raw material to produce superoxide dismutase compound enzyme and product:

[0032] liquid complex enzyme

[0033] 1. Air-dried corn grains with a weight of one ton and a germination rate of more than 70% (the germination rate is proportional to the enzyme yield) are first air-separated, then magnetically separated, placed in a germination tank, and 50-100ppm of sodium hypochlorite aqueous solution is added , so that the corn is submerged, stir well, and after about 30-60 minutes, release the sodium hypochlorite solution, add water and wash twice (test with PH test paper, no alkalinity is qualified, otherwise wash again). In the germination tank drained of water, add warm water at 28°C and keep it warm for 24 hours to make the corn kernels fully swell and release the remaining water. Gently ventilate the germination tank, keep warm at 28°C, and maintain saturated humidity to germinate corn and prevent anaerobic fermen...

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PUM

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Abstract

The method of producing superoxide dismutase complex enzyme at large scale with grain seeds features that the production process includes screening, disinfecting and washing grain seeds; soaking in hot water and germinating; grinding into coarse slurry and adding buffering liquid; adding cellulase, pulping into fine slurry and adding calcium chloride; separating liquid from solid, press filtering and centrifuging the filtrate to collect centrifuged liquid; ultrafiltering, adding amylase and concentrating with neutral ultrafiltering film to reach required SOD concentration. The present invention has the advantages of extracting SOD, peroxidase and hydrogen peroxidase, less impurity protein in product, high enzyme activity, etc.

Description

technical field [0001] The invention relates to a method for producing a superoxide dismutase compound enzyme, in particular to a method for producing a superoxide dismutase (SOD) compound enzyme and a series thereof on a large scale using five crop seeds of corn, wheat, rice, millet and sorghum product method. Background technique [0002] Superoxide dismutase (SOD) widely exists in organisms and was discovered by scientists in the 1960s. Its function is to remove superoxide free radicals that are highly toxic to organisms. Excessive free radicals are considered to cause cell aging to One of the leading causes of death. In recent years, some methods for preparing SOD have been developed and used in the fields of medicine, health products, food, cosmetics and daily chemicals, and achieved good results. In particular, it has good effects on anti-inflammatory, anti-aging, anti-radiation damage and inhibition of tumor growth. [0003] At present, SOD preparations are mostly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/20A61K9/48A61K38/44B01D61/14C12N9/02
Inventor 陈欣陈红漫曹淡君陈祖洁王成余
Owner 陈欣
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