Process for obtaining transgenosis plant by ovary drop

Abstract

A process for preparing the transgenic plant by ovary dripping includes such steps as pollination, germination of pullen particles, male nucleus coming in embryonary sac via pollen tube, cutting off style and 0-1.0 mm of ovary, and dripping exogenous DNA to direct transform reproductive cells or oospores.

Description

technical field

[0001] The invention belongs to the field of biological engineering. The invention relates to a gene transformation method for obtaining transgenic plants through ovary dripping, which can be applied to flowering plants without going through genetic transformation processes such as protoplast culture, cell culture, tissue culture and regeneration plants. Background technique

[0002] For a long time, in order to introduce exogenous functional genes into plants, people have explored a variety of transformation methods (Zhu Shengwei et al., Research progress in direct introduction of exogenous DNA into recipient plants, Bulletin of Botany, 2000, 17, 11-16), At present, two transformation methods mediated by gene gun and Agrobacterium are relatively perfect and widely used. In addition, the pollen tube passage method established by Chinese scholars [Zhou GY, Weng J, Introduction of exnogenous DNA into cotton embryos, Method in Enzymology, 1983, (101): 433-448] ...