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Microwave degradation method for polysaccharide

A microwave degradation and polysaccharide technology is applied in the microwave degradation field of polysaccharides to achieve the effects of less environmental pollution, improved bioavailability and simplified post-treatment

Inactive Publication Date: 2005-08-17
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of using microwaves to degrade polysaccharide macromolecules has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take an appropriate amount of Lycium barbarum polysaccharide with a number average molecular weight of about 20KDa, and make 150g.L -1 polysaccharide solution. Add an appropriate amount of acetic acid so that the concentration of acetic acid in the above solution is 0.3mol.L -1 , and stir well. Put this solution into a microwave generator, adjust the microwave power to 150W, degrade at 70°C for 1min, and then neutralize it to pH=7 with sodium hydroxide solution. Use an M1KDa dialysis bag, and use water dialysis as the mobile phase to remove the sodium acetate generated during the neutralization process. Freeze-dry the dialyzed internal solution to obtain oligomeric Lycium barbarum polysaccharide. The number-average molecular weight of the product tested by high-performance gel permeation chromatography (HPGPC) is 7KDa~11KDa, and the test conditions are chromatographic column: 7.8×300mm (Ultrahydrogel TM 120, 250, 1000, provided by Waters Corporation); column temper...

Embodiment 2

[0024] Take an appropriate amount of Lycium barbarum polysaccharide with a number average molecular weight of about 20KDa, and make 10g.L -1 polysaccharide solution. Add appropriate amount of acetic acid so that the concentration of acetic acid in the above solution is 2.5mol.L -1 , and stir well. Put this solution into a microwave generator, adjust the microwave power to 300W, degrade at 90°C for 5min, and then neutralize it to pH=7 with sodium hydroxide solution. Use an M1KDa dialysis bag, and use water dialysis as the mobile phase to remove the sodium acetate generated during the neutralization process. Freeze-dry the dialyzed internal solution to obtain oligomeric Lycium barbarum polysaccharide. The number average molecular weight measured by high performance gel permeation chromatography (HPGPC) is 1KDa~2KDa, and its testing conditions are the same as in Example 1.

Embodiment 3

[0026] Take an appropriate amount of chitosan with a number average molecular weight of about 500KDa, and make 5g.L -1 polysaccharide solution. Add appropriate amount of hydrochloric acid so that the concentration of hydrochloric acid in the above solution is 1.5mol.L -1 , and stir well. Put this solution into a microwave generator, adjust the microwave power to 250W, degrade at 80°C for 30min, and then neutralize it to pH=7 with sodium hydroxide solution. Use an M1KDa dialysis bag, and use water dialysis as the mobile phase to remove the sodium chloride generated during the neutralization process. Freeze-dry the dialyzed inner fluid to obtain oligomeric chitosan. The number average molecular weight measured by high performance gel permeation chromatography (HPGPC) is 3KDa~5KDa, and the test conditions are the same as in Example 1.

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Abstract

The microwave polysaccharide degrading process includes compounding polysaccharide solution of 5-150 g / L concentration and adding acid in concentration of 0.1-2.5 mol / L; setting the solution inside microwave generator and regulating power to 50-300 W to degrade at 10-90 deg.c for 1-30 min; and neutralizing pH to 7 with alkali, dialyzing to eliminate generated small molecular salt and freeze drying to obtain polysaccharide oligomer. The polysaccharide is originated from plant, animal and microbe and has numerically average molecular weight of 5-2000 KDa. The present invention has simple technological process, low cost and less pollution. The said technological process makes it possible to obtain various polysaccharide in specific molecular weight section and with greatly raised clinical bioavailability.

Description

technical field [0001] The invention relates to a microwave degradation method for polysaccharides. Background technique [0002] Polysaccharides are a class of natural products with various biological activities such as immune regulation, anti-tumor, anti-virus, anti-infection, and hypoglycemia. Studies have shown that some polysaccharides with specific molecular weight segments can effectively control a certain type of disease and its complications, but the content of polysaccharides with specific molecular weight segments in polysaccharides is very small. Therefore, degrading polysaccharide macromolecules into polysaccharides with specific molecular weight segments is an effective way to improve the bioavailability of polysaccharides. [0003] Traditional degradation methods mainly include acid degradation, photodegradation and enzymatic degradation. The acid degradation method is to dissolve polysaccharides in some inorganic acids such as phosphoric acid and hydrochlor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/28C08L5/00
Inventor 闫红毕雅静钟儒刚曾毅
Owner BEIJING UNIV OF TECH
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