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Collagen and its preparing process

A preparation process and collagen technology, applied in the field of collagen and its preparation process, can solve the problems of restricting the application space of fish skin collagen, destroying the characteristics and structure of collagen, fishy smell and poor water solubility, etc. Integrity, good flavor and taste, good solubility

Inactive Publication Date: 2005-10-19
青岛博恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the production technology of collagen at present, such as the alkali method cannot remove the oil in fish skin, the hydrolysis of acid-base method destroys the characteristics and structure of collagen, the existing various enzymatic hydrolysis methods are incomplete, the processing cost is high, and Collagen products still have obvious fishy smell and poor water solubility, etc., which affect the quality of collagen and greatly restrict the application space of collagen made from fish skin

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0008] The preparation process of the collagen protein of the present invention is to wash off the impurities on the fish skin with water and remove the fish scales, then clean it with water, then grind it with a meat blender, pour it into a container and add 1 / 10 to 1 / 5 of the weight of the fish skin Deionized water, heated to 50-60°C, using hydrolytic protease at pH 6.5-7.5, the amount of hydrolytic protease is 2-5‰ of the weight of fish skin, hydrolyzed for 1-2 hours, and carried out preliminary hydrolysis; then use flavor hydrolytic enzyme Secondary hydrolysis is carried out, the hydrolysis is at pH 6.0-8.0, the dosage of flavor hydrolase is 2-10‰ of the weight of fish skin, and the reaction time is 2-4 hours. After the hydrolysis is completed, heat to 85-90°C and keep for several minutes to fully inactivate. Stop heating, let the hydrolyzate stand for half an hour until a clear interface is reached, remove the upper layer of grease, filter the hydrolyzate, pump it into ca...

Embodiment 1

[0013] Weigh 1 kg of frozen cod skin and thaw it at room temperature until the whole fish skin has no lumps. Wash off the sundries attached to the fish skin with clean water, manually remove fish scales, fish meat and some fish oil, and then wash with deionized water. Grind it with a common meat blender, pour it into an enzymatic hydrolysis tank, add 100 grams of deionized water, heat it to 50°C, and use the enzyme Protamex to hydrolyze for 1 hour under the conditions of pH 7.0, enzyme dosage 2 grams, and The fish skin was initially hydrolyzed; then the enzyme Flavourzyme was used for secondary hydrolysis, the enzyme dosage was 4g, and the time was 3 hours. After the hydrolysis is complete, heat to 85°C for a few minutes. Stop heating, let the hydrolyzate stand for half an hour, reach a clear interface, and remove the upper layer of grease. After the hydrolyzate is filtered with a 800-mesh screen, it is pumped into carbon dioxide supercritical equipment for degreasing treatm...

Embodiment 2

[0015] Take by weighing 1 kilogram of frozen cod skins, and thaw at room temperature until the whole fish skin has no lumps. Wash off the sundries attached to the fish skin with clean water, and then rinse with deionized water. Crumble with a meat blender, pour into an enzymatic hydrolysis tank, add 200 grams of deionized water, heat up to 60°C, first use the enzyme Protamex under the conditions of pH 7.5, enzyme dosage 3 grams, and hydrolysis time of 2 hours to process the fish skin. The first hydrolysis; then the second hydrolysis with the enzyme Flavourzyme, the enzyme dosage is 3g, and the time is 4 hours. Usually, the hydrolysis is complete by heating until there are no small lumps, then stop heating, let the hydrolyzate stand for half an hour, remove the upper fish oil layer after clear stratification, then filter the hydrolyzate with a 400-mesh screen, and pump into the carbon dioxide supercharger The degreasing treatment is carried out in critical equipment, and the f...

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Abstract

The present invention relates to collagen and its preparation process. The preparation process includes eliminating foreign matter and scale from fish skin, washing, chopping in meat chopper, adding deionized water and heating inside a container, hydrolyzing with hydrolyzing proteinase, secondary hydrolyzing with flavoring hydrolase, heating to inactivate, letting stand to laminate, eliminating upper oil and grease layer, filtering the hydrolyze, defatting in a supercritical CO2 equipment, microfiltering to obtain filtrate, and spray drying to obtain collagen powder. The collagen powder has high solubility, high yield and good taste. The collagen may be used widely in food, cosmetics, medical supplementary material, enzyme and bioactive matter carrier, etc.

Description

technical field [0001] The invention relates to a new process for preparing collagen from fish skin-collagen and its preparation process. Background technique [0002] Collagen is a kind of protein. The English name "collagen" is derived from the Greek word, which means "substance that produces glue". It mainly exists in the skin, bone, tendon, ligament and blood vessel of humans and animals. , is the main component of connective tissue, plays a role in supporting organs and protecting the body. Collagen is the most abundant protein in mammals, accounting for 25-30% of the total protein in the body, equivalent to 6% of body weight. The amino acid composition of collagen is very special, among which glycine (Gly) accounts for 30%, proline (Pro) accounts for 12%, and hydroxyproline (Hypro) accounts for 9%. [0003] For a long time, collagen has been extracted from animal skin, Achilles tendon and other tissues. However, due to the hidden dangers and rejection reactions of vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
CPCY02P20/54
Inventor 张爱军沈继红
Owner 青岛博恩生物科技有限公司
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