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Inverse micelle method in use for degrading cellulose

A technology for degrading cellulose and reverse micelles, applied in the direction of fermentation, etc., can solve the problems of insufficient glucose yield and poor solubility, and achieve the effects of improving cellulose enzymatic hydrolysis efficiency, increasing concentration and reducing environmental pollution

Inactive Publication Date: 2005-11-16
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the enzymatic hydrolysis of cellulose has been carried out a lot of fruitful research, but the pollution-free new process of converting cellulose into glucose on an industrial scale has not yet been realized, mainly because natural cellulose is a hydrophobic substrate, The poor solubility in aqueous solution causes the yield of glucose in the traditional enzymatic hydrolysis method to be less than 50%.
At present, the degradation of macromolecular substances in the reverse micellar system is still a blank field

Method used

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  • Inverse micelle method in use for degrading cellulose
  • Inverse micelle method in use for degrading cellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Method for degrading microcrystalline cellulose using reverse micelles formed by surfactant AOT

[0022] 1. At room temperature (25°C), prepare 1ml of cellulase buffer solution with pH 6.0 (refer to the principle and application of biochemical technology for the preparation of buffer solution), and the concentration is 380μM, which is recorded as solution A;

[0023] 2. Dissolve 0.15mmol of 2-ethylhexyl sodium succinate (AOT) in 3ml of isooctane, shake vigorously and let it stand for later use, and record it as solution B;

[0024] 3. Take 14μl from solution A and mix with solution B, shake vigorously and then let it stand for 20 seconds. After it is uniform and transparent, it is a reverse micelle system containing cellulase molecules;

[0025] 4. After keeping the above system at 50°C for 10 minutes, add 10mg of microcrystalline cellulose, and fully react for 10 minutes in a constant temperature shaker (50°C, 220rpm);

[0026] 5. After that, add 140μl of phosph...

Embodiment 2

[0028] Example 2: Method for degrading microcrystalline cellulose using reverse micelles formed by the surfactant Triton X-100

[0029] 1. At room temperature (25°C), prepare 1ml of cellulase buffer solution with pH 6.0 (refer to the principle of biochemical technology and its application for the preparation of buffer solution), and the concentration is 400μM, which is recorded as solution A;

[0030] 2. Dissolve 0.15mmol of polyoxyethylene alkyl phenyl ether (Triton X-100) in 1ml of xylene, then add 2ml of n-hexanol, shake vigorously, and let it stand for use, which is recorded as solution B;

[0031] 3. Take 18μl from solution A and mix with solution B, shake it vigorously and let it stand for about 15 seconds. After it is uniform and transparent, it will be a reverse micelle system containing enzyme molecules;

[0032] 4. After keeping the system at 50°C for 10 minutes, add 10mg of microcrystalline cellulose, and fully react for 10 minutes in a constant temperature shaker (50°C...

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Abstract

An anti-micellar method for degradating cellulose includes dissolving cellulase in buffering liquid, preparing the organic solution of surfactant, mixing them together, adding microcrystalline cellulse, phosphoric acid buffering liquid and extractant, strong waving, centrifugal layering, and taking the upper soluiton, laying to obtain glucose solution.

Description

Technical field: [0001] The invention belongs to the field of bioenergy technology development and industrialization. Specifically, the invention relates to a reverse micelle method for degrading cellulose. This method applies reverse micelles to cellulase-catalyzed hydrolysis of cellulose, a biological enzyme-catalyzed reaction with important application prospects. The product glucose is further processed into products such as feed, food, chemical raw materials, microbial protein, amino acids, antibiotics, etc. Develop a new pollution-free biodegradation process of waste cellulose to realize the comprehensive utilization of cellulosic biomass (such as crop straw, etc.). The method effectively overcomes the defects of the traditional enzymatic hydrolysis method, and greatly improves the efficiency of cellulose degradation. Background technique: [0002] Reverse micelles are aggregates of surfactants in a solution containing a non-polar organic solvent as the bulk, with a polar ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B15/02
Inventor 梁毅陈楠范俊宝项瑾周拯陈杰
Owner WUHAN UNIV
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