Microcapsule preparing process utilizing waste beer yeast cell as wall material

A technology for beer waste yeast and yeast cells, which is applied in the field of microcapsule preparation, can solve the problems of high cost of microcapsules, difficult drying, difficult granulation operation, poor dispersibility, etc., and achieves reduced preparation cost, low cost of raw materials, and high entrapment rate. Effect

Inactive Publication Date: 2006-05-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But adopt this method to prepare microcapsules, generally all have special requirement to used yeast: as yeast needs through special culture condition, make intracellular fat content reach more than 40% (mass fraction) (U.S., USP4001480,1977); Microcapsule preparation process The yeast cells in the medium need to maintain biological activity (European Patent Office, EP0242135A2, 1987; United States, USP 5288632, 1994); and usually used in the form of wet cells, the water content of the cells is high, and the yeas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Wash the waste beer yeast recovered by the fermentation factory with 3 times of sterile water, pass through 80 mesh (diameter) sieves, remove impurities with larger particle diameters, and collect the thalline with 3000r / min centrifugation for 10min; follow the same steps Rinse the bacteria with sterile water for 2 times; collect the yeast precipitate, add water to make a 10% suspension; use a centrifugal atomizer with a rotation speed of 9500r / min, send the yeast suspension into a dryer for drying, and obtain Dry yeast granules with a diameter of about 0.5-2 mm and a moisture content of less than 2%.

[0013] Precisely weigh 2g of dry yeast, put it into a ground-mouthed Erlenmeyer flask, add 2.0g of cinnamaldehyde liquid, cover it and put it into a constant temperature water bath oscillator; shake it at 50°C (speed 80r / min) for 4h, and it can pass through the osmotic diffusion It enters the inside of yeast cells to form microcapsules; suction filters and reco...

Embodiment 2

[0015] Example 2 The purification and drying process of waste beer yeast is the same as in Example 1. Accurately weigh 2g of dry yeast, put it into a ground-mouthed Erlenmeyer flask, add 2g of clove oil, put it into a constant temperature water bath shaker after being capped; at 70°C (speed 80r / min) shake for 9h; suction filter and recover the unencapsulated clove oil; wash the essential oil remaining on the cell surface with absolute ethanol; vacuum dry at 30°C for 20min; collect the microcapsules and weigh them. The embedding rate of clove oil can reach 41.26%. Example 3 The purification and drying process of spent beer yeast is the same as in Example 1. Precisely weigh 2.0g of dry yeast, put it into a grinding-mouth Erlenmeyer flask, add 2.5g of rosemary essential oil, cover it and put it into a constant temperature water bath shaker; shake at 60°C (speed 100r / min) for 6h to form Microcapsules; suction filtration and recovery of unencapsulated essential oils; absolute etha...

Embodiment 4

[0016] Example 4 The purification and drying process of spent beer yeast is the same as in Example 1. Accurately weigh 2.0g of dry yeast, put it into a grinding mouth Erlenmeyer flask, add 2ml of 1mol / L ethanol solution of phenyl salicylate, cover it and put it in a constant temperature water bath shaker; Shake for 6 hours to form microcapsules; wash the solution remaining on the cell surface with absolute ethanol; vacuum dry at 40°C for 60 minutes; collect the microcapsules and weigh them. The embedding rate of phenyl salicylate can reach 67.64%.

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Abstract

The present invention discloses process of preparing microcapsule with waste beer yeast cell as wall material. Waste beer yeast recovered from fermentation plant is prepared into microcapsule through the steps of washing, sieving, centrifuging, etc. to purify; vacuum drying or spray drying to obtain dry yeast grains of 0.5-2 size and water content of 2 %; adding capsule core material in 1-4 times the dry yeast mass and vibrating at 30-80 deg.c for 4-12 hr to make the capsule core material penetrate into yeast cell; washing and drying. The present invention has the advantages of low cost, high core material embedding rate, fast embedding, and simple post washing and drying.

Description

technical field [0001] The invention relates to a method for preparing microcapsules by using waste beer yeast cells as wall materials, and belongs to a microcapsule preparation technology. Background technique [0002] Microcapsule technology is to use natural or synthetic polymer compounds as film-forming materials to prepare microcapsules. The substance enclosed in the microcapsule is called the capsule core, and the outer membrane formed by the film-forming material is called the capsule wall. Through microencapsulation, the core material can be protected from the influence of the external environment, reduce volatility, and improve stability, and microencapsulation also has the advantages of shielding odor, reducing toxicity, and controlling release. The preparation of microcapsules usually adopts three types of chemical methods, physical methods and physicochemical methods. However, these methods generally have limitations such as complex preparation process, high pr...

Claims

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Application Information

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IPC IPC(8): A61K9/50B01J13/02
Inventor 王金宇李淑芬
Owner TIANJIN UNIV
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