Monoamine oxidase activity determination method and monoamine oxidase diagnostic kit

A technology of monoamine oxidase and diagnostic kit, which is applied in the field of medical inspection and measurement, can solve the problems of difficult measurement, inability to apply automatic biochemical instrument analysis, and limited practicability, and achieve credible detection results, low test cost, and high accuracy. Effect
CN1789428AInactive Publication Date: 2006-06-21SUZHOU ANJ BIOTECHNOLOGY CO LTD
0 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2006-06-21
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention relates measuring method of monoamine oxidase activity and its diagnostic kit. The invention comprises the following steps: using the monoamine oxidase to hydrolyze benzylamine, p-toluidine red-ª‰-azo naphthol, butyl amine, amyl amine, ª‰-phenylethylamine, tyramine and other aminated compounds to produce the ammonia, then the ammonia carried out coupling reaction with glutamate dehydrogenase to turn the oxidation type coenzyme to reduction type coenzyme, detecting the reducing velocity of absorbance of main wavelength 340nm to reflect the activity of monoamine oxidase in sample by definite quantity. The method has high specificity and good accuracy. The diagnostic kit is made to double agents or tri-agent to reduce the across impact and keep the agent stability and be good for long term storage. The method can be rapidly detected by the ultra-violet / visible light analysis meter or semi-automatic / full-automatic biochemical analysis meter, so the method is easy to spread and use.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for measuring monoamine oxidase activity by an enzymatic method and a monoamine oxidase diagnostic kit prepared by applying the method, which belongs to the technical field of medical inspection and measurement. Background technique

[0002] In the prior art, the methods for measuring the activity of monoamine oxidase (MAO) mainly include: fluorescence method, immunosuppression method and chemical spectrophotometry. The fluorescence method uses β-phenylethylamine as a substrate to detect monoamine oxidase, and the basis is that the reaction rate of β-phenylethylamine is the highest at 10-100 mg, which is higher than that of benzylamine and 5-hydroxytryptamine. The immunological method uses monoamine oxidase antibody to react with monoamine oxidase to measure the monoamine oxidase isoenzyme formed after the reaction. Currently, the most widely used monoclonal antibodies are MAO-A3C9, MAO-A4F10, MAO-A7B10, MAO-A7E10 and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More