45results about How to "Guaranteed application test results" patented technology

The invention relates to a sodium / potassium ion ratio detecting method, a sodium / potassium ion ratio detecting system and a sodium / potassium ion ratio detecting kit. The invention relates to a method for detecting a sodium / potassium ion ratio concentration. The method comprises the following steps: (1) preparing multiple solution samples with different sodium / potassium ion ratios; (2) putting the multiple solution samples under a fluorescence spectrophotometer, and testing fluorescence intensity values at a first wavelength and a second wavelength; (3) acquiring a standard curve of the sodium / potassium ion ratios; (4) adding DNA molecules with fluorescently-labeled groups and can form different G-four chain structures in the presence of sodium and potassium ions respectively into a liquid sample to be tested, and regulating pH value of the sample so as to obtain a test solution; (5) putting the test solution under the fluorescence spectrophotometer, and testing fluorescence intensity values at the first wavelength and the second wavelength; and (6) finding a corresponding sodium / potassium ion concentration ratio of the test solution in the standard curve of the sodium / potassium ion ratios according to a ratio of the fluorescence intensity value tested at the first wavelength to the fluorescence intensity value tested at the second wavelength in the step (5).

The invention relates to a method for detecting a ratio of sodium ions to potassium ions, a kit and a system. The invention relates to a method for detecting mol ratio of sodium ions to potassium ions in solution, comprising the following steps: (1) preparing a plurality of solution samples according to difference of ratios of sodium ions to potassium ions, wherein each solution sample contains cyanine dye with the same concentration and DNA molecules capable of respectively forming different G-quadruplex structures with the existence of sodium ions and potassium ions; (2) detecting fluorescence intensity level where wavelength is collected; (3) plotting by using the ratio of sodium ions to potassium ions in each solution sample as a horizontal ordinate or a vertical ordinate and using the fluorescence intensity level where wavelength is collected detected in the step (2) as a horizontal ordinate or a vertical ordinate so as to obtain a specification curve of ratios of sodium ions to potassium ions; (4) adding the DNA molecules in a liquid sample to be detected, and adjusting the pH value so as to obtain a test solution; (5) detecting the fluorescence intensity level of the wavelength where the wavelength is collected; and (6) finding out corresponding ratio of sodium ions to potassium ions in the test solution in the specification curve of ratios of sodium ions to potassium ions.

The invention relates to a kind of enzyme colorimetric method and a method of the coupling method measuring the density of citric acid and a diagnosis reagent box applying the citrate synthetase and coupling malic dehydrogenase enzymatic reaction continues monitoring method / ratio colorimetric method. The reaction of citrate synthetase enzymolysis citric acid generates oxaloacetic acid and then through the effect of coupling malic dehydrogenase at last oxidizes the reduced coenzyme (there is a absorption peak at the site of 340nm) becoming the coenzyme (there is a absorption peak at the site of 340nm) so we can assay the degree / speed of the fall-way absorbance at the place of 340nm. It can measure and calculate the density of citric acid by measuring the degree / speed of the fall-way absorbance at the place of 340nm. The method has high specificity and it is not polluted by the endogenous and exogenous object and the test result is precise and accurate. The invention can get the array result by the ultraviolet / visible light analytic instrument so it is convenience to extend and apply.

This invention disclosed a determination method for paraoxonase activity and the reagent box. The procedures are as follows: react with phenylacetate to produce acetic acid, oxidate the deoxidize style coenzyme to coenzyme, measure the coenzyme at 340nm absorbency, calculate the activity and concentration. The reagent box contains buffer, deoxidize style coenzyme, phenylacetate and stabilizer. The result obtained by this method was precise and accurate.

The invention relates to enzyme method measuring zinc ion concentration and zinc ion diagnose kit. The invention uses alkaline phosphatase reaction colorimetry method that uses alkaline phosphatase to enzymolysis four-nitrophenol phosphoric acid to form into four-nitrophenol under the activation of zinc ion, by detecting the ascending speed of 405nm dominant wavelength absorbance the zinc ionic content in sample would be quantitatively responsed. The method has high specificity, has no influence from inside and outside metal material, accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet / visible light, analyzer or semi-transfer / full automatic biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.

The invention is about the enzyme method of measuring magnesium ion and its diagnosis reagent box. Making use of the peculiarity that magnesium ion in the sample of plasma or serum and so on can activate the activation of glycerokinase, and producing glycerol-3-phosphoryl by reacting glycerol under the existence of adenosine triphosphate, and then causing coupled reaction with glycerol-3-phosphoryl dehydrogenase and transferring oxidized coenzyme to reduced coenzyme. Testing the descending range of dominant wave-length340nm absorbance and finally measuring the content of magnesium ion in the sample. This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet / visible light analyzer or semiautomatic / automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.