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Lichen spore bacillus strain and application of bacillocin produced by it

A technology of Bacillus licheniformis and bacteriocin, applied in the direction of bacteria, microorganism-based methods, applications, etc., can solve the problem of trypsin insensitivity

Inactive Publication Date: 2006-06-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But none of them are antagonistic to Staphylococcus aureus and fungi, and are insensitive to trypsin

Method used

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  • Lichen spore bacillus strain and application of bacillocin produced by it

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Experimental program
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Embodiment Construction

[0016] 1. Test materials:

[0017] 1.1 Antagonist

[0018] Bacillus licheniformis ZJU12 was isolated from the soil of Huajiachi Campus of Zhejiang University by our laboratory. The culture conditions were LB liquid or solid medium (peptone 10g / L, NaC110g / L, yeast extract 5g / L, solid plus agar 15-20g / L, pH7.0), 30°C, aerobic.

[0019] 1.2 Pathogenic bacteria

[0020] Acinetobacter baumanii, Bacillius subtilis, Branhamella catarrhalis, Enterobacter cloacae, Enterococcus faecium, Escherichia coli , Micrococcus flavus, Proteus sp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis. The culture condition is LB liquid or solid medium, 30°C, aerobic.

[0021] Alternaria brassicae, Xanthomonasoryzae pv.oryzae, Pyricularia grisea Sacc., Fusarium oxysporum, Rhizoctonia solani, Dendrobium black spot pathogen (Guignardia sp), the culture condition is PSA solid medium (potato 200g / L, sucrose 10g / L, agar 15-20g / L, pH7.0), 30 ℃, aerobic.

[0022] The pathogenic ...

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Abstract

The invention discloses bacillus licheniformis strain. Its preservation name is Bacillus licheniformis ZJU12. The preservation unit is Chinese typical culture preservation center. The data is July fourth in 2005 yare. The number is CCTCC M 205072. The invention also discloses the produced bacteriocin which has antagonism for gram-positive bacteria, plant pathogenic fungi, plant pathogeny gram-negative bacteria.

Description

technical field [0001] The invention relates to a bacillus licheniformis strain and the application of the bacteriocin produced by the bacillus licheniformis in food biological antisepsis and preservation, animal and plant disease prevention and control, medicine and the like. Background technique [0002] Bacillus licheniformis is a kind of facultative anaerobic saprophytic bacteria widely existing in nature. For a long time, this bacterium has been used as an industrial production bacterium for proteolytic enzymes, amylases, bioactive agents, antibiotics and other special chemicals, and has very low toxicity to human health and the environment. The peptide antagonist produced by Bacillus licheniformis was first isolated by Johnson et al. in 1945 from a strain of Bacillus subtilis var Tracy (Meleney changed its name to Bacillus licheniformis Var Tracy in 1994), and it was named as bacitracin. (Bacitracin), has been widely used in the fields of medicine and veterinary medic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C12P1/04C12R1/10
Inventor 何李黎陈卫良
Owner ZHEJIANG UNIV
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