Method for detecting 19-nortestosterone and special enzyme-linked immune reagent kit thereof

An enzyme-linked immunosorbent reagent and a technology of nortestosterone, which is applied in the field of detection of 19-nortestosterone, can solve the problems of cumbersome process, unsuitability for on-site monitoring and screening of a large number of samples, complex equipment, etc., and achieve simple sample pretreatment process, The inspection method is convenient and easy to implement, and the effect of high accuracy is
CN1811443AInactive Publication Date: 2006-08-02CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2006-08-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a method for detecting 19-nandrolone and its special-purpose ELIA kit. Said kit includes 19-nandrolone specific antibody, coating source and enzyme label. The described coating source is conjugate of 19-nandrolone semiantigen and carrier protein or antiantibody. The described enzyme label is enzyme-labelled antiantibody or enzyme-labelled 19-nandrolone semiantigen. When the described coating source in the conjugate of 19-nandrolone semiantigen and carrier protein, the described enzyme label is enzyme-labelled antiantibody, and when the described coating source is anti-antibody, the described enzyme label is the enzyme-labelled 19-nandrolone semi-antigen.
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Description

technical field

[0001] The invention relates to a method for detecting 19-nortestosterone and a special ELISA kit. Background technique

[0002] 19-nortestosterone (19-nortestosterone, NT) is a synthetic hormone that has androgenic effects and belongs to anabolic steroids. Clinically, it is mainly used to treat anemia and senile osteoporosis. It can also promote muscle growth and development, increase training endurance and training load. However, it also has very serious side effects. Ingestion by the mother will masculinize the female fetus and cause reproductive deformities. Long-term use by women will produce masculine features, and long-term use by men will cause premature baldness. Short-term high-dose use can lead to liver dysfunction, and may also lead to cancer, diabetes, mental disorders, etc. Therefore, it is necessary to strengthen the detection of this drug.

[0003] The chemical methods for detecting the residual amount of 19-nortestosterone mainly include ...

Claims

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