Human alpha-lacto albumin gene transgenic cloned macro domectic animal production method

A technology of whey protein and production method, which is applied in the fields of transgene-cloning and bioengineering, and can solve the problems of α-lactalbumin full-genome DNA transgene report, inconvenient gene operation, and impact.

Inactive Publication Date: 2006-10-25
李宁
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1999, after doing a lot of analysis and research work by Fujiwara et al., they believed that the human α-lactalbumin gene containing 50kb 5'flanking region and 50kb 3'flanking region could obtain stable expression that was not affected by the position effect, but only contained Transgene expression in the 20kb 5' and 20kb 3' flanking regions is affected by position effects
In 2003, Liu Siguo and others used the 7.3kb 5' regulatory region of human α-lactalbumin gene, the 2.0kb coding region and the 227bp bovine growth hormone 3' regulatory region to c

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human alpha-lacto albumin gene transgenic cloned macro domectic animal production method
  • Human alpha-lacto albumin gene transgenic cloned macro domectic animal production method
  • Human alpha-lacto albumin gene transgenic cloned macro domectic animal production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] 1 Experimental materials

[0053] 1.1 Plasmids and strains

[0054] Host bacteria Escherichia coli DH5α and DH10B, pGEM-5Zf, pGEM-T were purchased from Promega Company, pCMV-EGFP-IRES-NEO and pIRES-NEO were purchased from Clontech Company

[0055] 1.2 Experimental animals

[0056] The 3-month-old fetuses of Holstein dairy cows were obtained from slaughterhouses, and the ovaries of yellow cattle were obtained from slaughterhouses in surrounding areas of Beijing.

[0057] 1.3 Main reagents

[0058] DMEM / F 12 Powder, DMEM powder medium, trypan blue (trypan blue), TCM199 powder, glutamine and G418 are products of Gibco Company; fetal bovine serum is product of Hyclone Company; dNTP and PCR primers were purchased from Shanghai Bioengineering Company; Taq enzyme was purchased from In the Agricultural Biotechnology Laboratory of China Agricultural University; endonuclease SspI and BamHI are products of Huamei Bioengineering Company; IPTG, X-gal, ampicillin (Amp), kanamycin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention deals with the method of cloning the big critter using the transferring human a-whey albumen gene, the handling process is showed as follows: (1) using whole Human a-whey albumen gene frame to construct the galactophore unusual expressing carrier, (2) constructing the in series fabric connecting the human a-whey albumen unusual expressing carrier and the double single choosing carrier and transmitting the in series fabric DNA to the body nucleolus to gain the transferring gene cell, (3) cloning the body cell using the transmitting gene cell for the nucleus feeding body to gain the transferring clone big critter containing the transferring human a-whey albumen. The transferring gene milch cow galactophore unusually expressing recombining human a-whey albumen can be tapped the health caring drug and the leechdom, the milk containing the recombining human a-whey albumen can be right tapped to the health care milk and the milk produce with the high appending value.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to the field of transgene-cloning technology. Background technique [0002] The research on transgenic animals means that human beings change the genetic composition of animals purposefully, planned, based, and predictably according to their own wishes, and the purposes of changing their genetic composition are various. For example, geneticists hope to observe phenotypic changes by changing the genetic composition of animals, and physiologists hope to study the impact of a gene on the physiological state of the body through the expression of a specific gene. Transgenic research related to animal production hopes to endow animals with new phenotypic traits through transgenic technology. The research relies on molecular biology, animal embryo and gamete manipulation techniques in experimental techniques. [0003] At present, the production methods of transgenic animals mainly include m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/09C12N15/12C12N15/65C12N15/85
Inventor 汤波戴蕴平龚国春曹更生于舒洋张磊李宁
Owner 李宁
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap