Human alpha-lacto albumin gene transgenic cloned macro domectic animal production method

Abstract

The invention deals with the method of cloning the big critter using the transferring human a-whey albumen gene, the handling process is showed as follows: (1) using whole Human a-whey albumen gene frame to construct the galactophore unusual expressing carrier, (2) constructing the in series fabric connecting the human a-whey albumen unusual expressing carrier and the double single choosing carrier and transmitting the in series fabric DNA to the body nucleolus to gain the transferring gene cell, (3) cloning the body cell using the transmitting gene cell for the nucleus feeding body to gain the transferring clone big critter containing the transferring human a-whey albumen. The transferring gene milch cow galactophore unusually expressing recombining human a-whey albumen can be tapped the health caring drug and the leechdom, the milk containing the recombining human a-whey albumen can be right tapped to the health care milk and the milk produce with the high appending value.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to the field of transgene-cloning technology. Background technique [0002] The research on transgenic animals means that human beings change the genetic composition of animals purposefully, planned, based, and predictably according to their own wishes, and the purposes of changing their genetic composition are various. For example, geneticists hope to observe phenotypic changes by changing the genetic composition of animals, and physiologists hope to study the impact of a gene on the physiological state of the body through the expression of a specific gene. Transgenic research related to animal production hopes to endow animals with new phenotypic traits through transgenic technology. The research relies on molecular biology, animal embryo and gamete manipulation techniques in experimental techniques. [0003] At present, the production methods of transgenic animals mainly include m...

AI Technical Summary

Problems solved by technology

In 1999, after doing a lot of analysis and research work by Fujiwara et al., they believed that the human α-lactalbumin gene containing 50kb 5'flanking region and 50kb 3'flanking region could obtain stable expression that was not affected by the position effect, but only contained Transgene expression in the 20kb 5' and 20kb 3' flanking regions is affected by position effects

In 2003, Liu Siguo and others used the 7.3kb 5' regulatory region of human α-lactalbumin gene, the 2.0kb coding region and the 227bp bovine growth hormone 3' regulatory region to construct vectors. Among the five transgenic mice, only One mouse can detect the expression of human α-lactalbumin (0.3mg / ml), indicating that this expression vector is not very ideal

Using genomic DNA as the target gene to make transgenic animals can achieve higher expression efficiency than cDNA sequences[5], but too large fragments are not convenient for gene manipulation, so it is of great significance to obtain high-efficiency expression small-structure transgenic vectors

So far, there have been no transgenic reports of α-lactalbumin whole genome DNA

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