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Antibiotic in lactam class, and prepartion method

A technology of lactams and antibiotics, applied in the field of antibiotics, to achieve the effects of simple fermentation and post-treatment process, excellent antibacterial activity, and cheap and easy-to-obtain raw materials

Inactive Publication Date: 2010-04-07
海南博士威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing problem is that due to long-term and extensive use, many germs, such as Staphylococcus aureus, Streptococcus, pneumococcus, etc., have produced obvious drug resistance to existing β-lactam antibiotics, so it is urgent to research and develop new ones. Antibiotics, especially those with novel molecular structures

Method used

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  • Antibiotic in lactam class, and prepartion method
  • Antibiotic in lactam class, and prepartion method
  • Antibiotic in lactam class, and prepartion method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Compound Preparation

[0032] 1 Fermentation

[0033] (1) Seed culture medium (8g of glucose, 8g of soluble starch, 6g of beef extract, 15g of peptone, 5g of sodium chloride, 1000ml of distilled water, pH 7.2), use a 250ml triangular flask to dispense 100ml per bottle, and then use 1ml of The bacterial water washes the spores of Streptomyces qinlings off the slope and makes a spore suspension, so that the concentration is 1 × 10 6 ~1×10 7 spores / ml, add 1ml of spore suspension to each bottle, and place on a shaker for 24h incubation at 30±1℃;

[0034] (2) Fermentation adopts liquid shake flask fermentation, the culture medium (10g of millet, add 1000ml of distilled water, boil for 15min, filter out the rice grains, add 15g of glucose, 2g of calcium carbonate, 5g of sodium chloride, 5g of peptone, pH 7.2) at 1×10 5 Sterilize under Pa for 30min, and divide into 250ml conical flasks, each 50ml. The inoculum amount was 10% (V / V), the shaking speed was 210r / mi...

Embodiment 2

[0052] Example 2: Determination of Antibacterial Activity

[0053] 1. Determination of antibacterial activity of Qinlingmycin against common bacteria

[0054] The antibacterial activity of Qinlingmycin was determined by the tube-disk method. The bottom medium is 2% agar medium, and the upper medium is beef extract medium. Add 10ml of the bottom medium to each petri dish, and after condensation, pour the melted upper medium (add the test bacteria) to the surface of the solidified bottom medium, and then place the Oxford cup after condensation. . 0.2 ml of Qinlingmycin liquid was added to each Oxford cup, and each treatment was repeated 3 times, and clear water was used as a control. Incubate in an incubator at 26±1℃ for 24h, and measure the diameter of the inhibition zone. The results are shown in Table 1:

[0055] Table 1 Antibacterial activity of Qinlingmycin

[0056]

[0057] As can be seen from Table 1, Qinlingmycin has significant inhibitory effects on both Gram-p...

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Abstract

This invention discloses a method for preparing lactam antibiotic from Streptomyces qinlingnensis sp.nov (preserved as CGMCC No.1831). The method comprises: (1) culturing Streptomyces qinlingnensis sp.nov strains, and fermenting in a liquid; (2) filtering, adsorbing by a macroporous adsorption resin, and eluting with methanol; (3) performing chromatographic separation with a silica gel chromatographic column to obtain crude crystal, and purifying by high performance liquid chromatography to obtain the high purity product. The antibiotic has a wide spectrum antibacterial performance, and can inhibit a variety of pathogenic bacteria.

Description

technical field [0001] The invention belongs to the field of antibiotics, in particular to a lactam antibiotic and a preparation method thereof Background technique [0002] β-lactam antibiotics are commonly used antibiotics in clinical practice. They are widely used because of their broad antibacterial spectrum, obvious antibacterial effect and definite curative effect. The current problem is that many bacteria, such as Staphylococcus aureus, Streptococcus, Pneumococcus, etc., have obvious resistance to the existing β-lactam antibiotics due to long-term extensive use. Therefore, there is an urgent need to research and develop new Antibiotics, especially those with completely new molecular structures. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to overcome the deficiencies existing in the prior art, and provide a lactam antibiotic, which has a brand-new molecular structure and has high inhibitory activity against various pathogenic bacteria....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/04C12P17/18A61K31/551A61P31/04C07D205/00C07D243/00
CPCY02A50/30
Inventor 姬志勤吴文君龙建友师宝君祁志军胡兆农
Owner 海南博士威生物科技有限公司
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