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Antibiotic in lactam class, and prepartion method

A technology of lactams and antibiotics, applied in the field of antibiotics, to achieve the effects of excellent antibacterial activity, cheap and easy-to-obtain production raw materials, and simple fermentation and post-treatment processes

Inactive Publication Date: 2006-12-06
海南博士威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing problem is that due to long-term and extensive use, many germs, such as Staphylococcus aureus, Streptococcus, pneumococcus, etc., have produced obvious drug resistance to existing β-lactam antibiotics, so it is urgent to research and develop new ones. Antibiotics, especially those with novel molecular structures

Method used

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  • Antibiotic in lactam class, and prepartion method
  • Antibiotic in lactam class, and prepartion method
  • Antibiotic in lactam class, and prepartion method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: compound preparation

[0032] 1 fermentation

[0033] (1) Seed culture medium (glucose 8g, soluble starch 8g, beef extract 6g, peptone 15g, sodium chloride 5g, distilled water 1000ml, pH value 7.2), use 250ml triangular flasks to pack 100ml in each bottle, and then use 1ml without Bacterial water Wash the spores of Streptomyces qinlingensis on the slope and make a spore suspension to make the concentration 1×10 6 ~1×10 7 Individuals / ml, add 1ml spore suspension to each bottle, place on a shaker and incubate at 30±1°C for 24h;

[0034] (2) Fermentation Use a liquid shake flask for fermentation. The culture medium (10g millet, add distilled water 1000ml, boil for 15min, filter out rice grains, add glucose 15g, calcium carbonate 2g, sodium chloride 5g, peptone 5g, pH value 7.2) in 1 × 10 5 Sterilize under Pa for 30 minutes, and pack in 250ml Erlenmeyer flasks, 50ml per bottle. The inoculum size is 10% (V / V), the shaker speed is 210r / min, and the culture i...

Embodiment 2

[0052] Embodiment 2: Determination of antibacterial activity

[0053] 1. Determination of the antibacterial activity of Qinlingmycin against common bacteria

[0054] The antibacterial activity of Qinlingmycin was determined by the tube-and-disc method. The bottom medium is 2% agar medium, and the upper medium is beef extract medium. Add 10ml of the bottom culture medium to each petri dish, after condensation, pour the upper culture medium (adding the bacteria to be tested) that has melted and cooled to about 45°C into the surface of the solidified bottom culture medium, and place the Oxford cup after condensation . Add 0.2ml of qinlingmycin drug solution into each Oxford cup, and repeat each treatment 3 times, and clear water is used as a control. Incubate in an incubator at 26±1°C for 24 hours, and measure the diameter of the inhibition zone. The results are shown in Table 1:

[0055] Pathogens tested

Concentration (mg·L -1 )

Antibacterial zone diamete...

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Abstract

This invention discloses a method for preparing lactam antibiotic from Streptomyces qinlingnensis sp.nov (preserved as CGMCC No.1831). The method comprises: (1) culturing Streptomyces qinlingnensis sp.nov strains, and fermenting in a liquid; (2) filtering, adsorbing by a macroporous adsorption resin, and eluting with methanol; (3) performing chromatographic separation with a silica gel chromatographic column to obtain crude crystal, and purifying by high performance liquid chromatography to obtain the high purity product. The antibiotic has a wide spectrum antibacterial performance, and can inhibit a variety of pathogenic bacteria.

Description

technical field [0001] The invention belongs to the field of antibiotics, in particular to a lactam antibiotic and a preparation method thereof Background technique [0002] β-lactam antibiotics are commonly used in clinical practice. They are widely used for their wide antibacterial spectrum, obvious antibacterial effect and definite curative effect. The existing problem is that due to long-term and extensive use, many germs, such as Staphylococcus aureus, Streptococcus, pneumococcus, etc., have produced obvious drug resistance to existing β-lactam antibiotics, so it is urgent to research and develop new ones. Antibiotics, especially antibiotics with completely new molecular structures. Contents of the invention [0003] The purpose of the present invention is to overcome the shortcomings in the prior art and provide a lactam antibiotic with a brand new molecular structure and high inhibitory activity against various pathogenic bacteria. [0004] Another object of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04C12P17/18A61K31/551A61P31/04C07D205/00C07D243/00
CPCY02A50/30
Inventor 姬志勤吴文君龙建友师宝君祁志军胡兆农
Owner 海南博士威生物科技有限公司
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