Method for preparing ginseng anti-cancer active component by immobilized enzyme

Abstract

The immobilized enzyme process for preparing effective anticancer ginseng component includes the steps of immobilizing industrial enzyme preparation, converting immobilized enzyme and collecting converted product; and features that the glycosyl radical in diol type ginsenoside molecule is fixed point excised by the immobilized enzyme to form the target compound C-K. The process is superior to available process, which has environmental pollution, drastic reaction, serious damage on aglycone, low product yield and other demerits. The process of the present invention is simple, low in cost and high in efficiency, and may be applied in preparing great amount of C-K for preparing antitumor medicine.

Description

(1) Technical field: [0001] The present invention relates to the method for preparing C-K by converting panaxadiol group saponin by immobilized industrial enzyme preparation, particularly relate to industrial enzyme preparation is β-glucosidase, cellulase, cellobiase; The immobilization method used is seaweed acid calcium method. (two) background technology: [0002] Immobilization technology refers to the positioning of free cells or enzymes in a limited space area by chemical or physical means, so that they can maintain activity, have a long acting time, are not easy to lose, have significantly increased anti-toxicity and tolerance, and are easy to separate solids and liquids. , and can be used repeatedly. It began in 1916 when Nelson and Griffin discovered that invertase adsorbed on bone carbon particles still maintained the same activity as free enzymes. After that, people continued to study various enzyme immobilization techniques and methods, and successively realized...

AI Technical Summary

Problems solved by technology

[0007] Although these two methods can hydrolyze saponins, their hydrolysis conditions are very severe and difficult to control, and because saponins can undergo dehydration, cyclization, double bond displacement, substituent displacement, and configuration transformation during the hydrolysis process and other changes, so that the by-products of hydrolysis increase, the target product is difficult to obtain or the content is low

More importantly, these two methods all discharge a large amount of pollutants such as organic solvents, acids and alkalis to the environment from raw materials to the reaction process, which is harmful to the environment to a large extent; and acids and alkalis are also harmful to the operators themselves. It will cause harm, so it is necessary to find new conversion technologies and methods, that is, to more effectively convert low-activity, high-content plant saponins into high-activity, low-natural content monomeric saponins, and to achieve no burden on the environment , which is a feasible way to make rational use of natural medicine resources, implement clean production, bring Chinese medicine to the world, and establish a green Chinese medicine industry