Anopheline of microbe prepared from starch wastewater, and preparation method

A technology for starch wastewater and microorganisms, applied in the fields of botanical equipment and methods, biocides, animal repellants, etc., to achieve the effect of breaking through limitations, reducing wastewater treatment costs, and expanding resource utilization approaches

Inactive Publication Date: 2007-02-21
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simultaneous Treatment of Starch Wastewater During the Preparation of Mosquito Killer

Method used

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  • Anopheline of microbe prepared from starch wastewater, and preparation method
  • Anopheline of microbe prepared from starch wastewater, and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 produces Bs mosquito killer

[0033] (1) Starch wastewater sample

[0034] Take starch wastewater from a starch factory in Beijing. The factory uses corn as raw material to produce starch. The processing process is: corn→sieving→soaking→crushing→germ separation→grinding→sieving→precipitation separation→starch washing→dehydration→ Dry → starch. The main sources of wastewater are soaking water and yellow slurry water. After analysis, the carbohydrate content in the starch wastewater was 6.8g / L, the crude protein content was 19.0g / L, the solid content was 8.61%, and the pH=4.80.

[0035] (2) Conditioning of wastewater medium

[0036] To adjust the solid content of starch wastewater to 5.0%, add 0.75g / L dipotassium hydrogen phosphate, 0.30g / L magnesium sulfate and 2.5g / L foam enemy. Adjust the pH to 9.5 with NaOH, sterilize in the seed tank and fermenter at 15 lb pressure and 121 °C for 60 minutes, cool to 35 °C for later use, and the pH after sterilization...

Embodiment 2

[0050] Embodiment 2 produces Bs mosquito killer

[0051] (1) Starch wastewater sample

[0052] The starch wastewater sample is the same as in Example 1.

[0053] (2) Conditioning of wastewater medium

[0054] To adjust the solid content of starch wastewater to 3.0%, add 0.50 g / L dipotassium hydrogen phosphate, 0.20 g / L magnesium sulfate and 5.0 g / L peanut oil. with Ca(OH) 2 Adjust the pH to 9.0, sterilize in the seed tank and fermenter at 15 lb pressure and 121°C for 60 minutes, cool to 35°C for later use, and the pH after sterilization is about 7.5.

[0055] (Other defoamers, such as foam enemy, soybean oil, polypropylene glycol, etc., replace peanut oil respectively, and the effect is the same.)

[0056] (3) Shake flask culture

[0057] The fermentation strain was changed from Bs 1593 to Bs 2362 strain, and the rest were the same as in Example 1.

[0058] (4) Seed tank fermentation

[0059] Inoculate 1.0% Bs 2362 shake flask bacterial solution, and ferment and cultiva...

Embodiment 3

[0067] Embodiment 3 produces Bti mosquito killer

[0068] (1) Starch wastewater sample

[0069] The starch wastewater from a starch factory in Shijiazhuang City, Hebei Province is taken. The factory uses sweet potatoes as raw materials to produce starch. The processing process is: sweet potatoes→washing→grinding→sieving→slurry→centrifugal separation→starch washing→dehydration→drying→starch. The main sources of wastewater are washing water and separation wastewater (potato pulp wastewater and protein water). After analysis, the carbohydrate content in the wastewater was 13.2g / L, the crude protein content was 6.1g / L, the solid content was 7.10%, and the pH=4.45.

[0070] (2) Conditioning of wastewater medium

[0071] Adjust the solid content of starch wastewater to 2.5%, add mineral salt 0.4g / L dipotassium hydrogen phosphate, 0.35g / L magnesium sulfate, and 2.0g / L defoamer polypropylene glycol. with Ca(OH) 2 Adjust the pH to 9.7, sterilize in the seed tank and fermenter at 15...

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Abstract

A microbial mosquito killer is prepared from the starch sewage through preparing culture medium, culturing in shake flask, fermenting in seeding tank, fermenting in fermentor, post-treating of fermented liquid and quality examination of product.

Description

technical field [0001] The invention relates to a mosquito killer, in particular to a microbial mosquito killer prepared from starch wastewater and a preparation method thereof. Background technique [0002] Starch wastewater is a kind of high-concentration organic wastewater produced in the process of starch processing. These wastewater contain a large amount of residual starch, glucose, fructose, maltose and other carbohydrates, proteins and organic acids, etc., and are easily corrupted and smelly. The COD is usually at 2000 Between -20000mg / L, it is one of the most polluted wastewater in the food industry. [0003] The main raw materials for starch processing are corn (accounting for about 80%), potatoes, wheat and other starch-rich plant roots, and the waste water mainly comes from the soaking and starch separation section. There are nearly 600 starch production enterprises in my country, and the annual discharge of wastewater exceeds 100 million tons. Most of the orga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P7/04
Inventor 周顺桂雷发懋王跃强
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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