Method for preparing fermention medium, and producing biological pesticide by using wastewater from fermentation
A technology for fermenting medium and fermenting waste water, which is applied in the field of biopesticide production, and can solve problems such as failure to meet requirements, environmental pollution, and waste of resources, and achieve the effects of low pollution probability, good antibacterial effect, and low cost
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Embodiment 1-3
[0023] The preparation of the seed medium used: biogas slurry 200ml, water 800ml, glucose 18g, K 2 HPO 4 0.4g, adjust the pH to 7.0 before sterilization.
[0024] The preparation of the fermentation medium used: biogas slurry 2.4L, water 9.6L, glucose (sucrose) 240g, urea 140g, K 2 HPO 4 9.5.0g, CaCO 3 5.0g, MgSO 4 4.5g, KCl 3.5g and other inorganic salts, vitamin C 2.0g, ZnSO 4 1.0g, FeCl 3 1.0g, adjust the pH value to 6.9-7.1 before sterilization.
[0025] Seed culture: Inoculate milky yellow bacteria such as Streptomyces griseus, Xanthomonas campestris pv.oryzae, Bacillus subtilis and other milky yellow bacteria into the seed medium respectively, and set the temperature at 100rpm and 28°C Shaking table shaking culture for 4 days, as a first-class seed fungus, similarly, the first-class seed fungus is expanded and cultivated according to the ratio of "first-class seed fungus: strain culture solution = 1: 10", as a second-class or large-scale seed fungus. Cultur...
Embodiment 4-6
[0028] The fermentation medium used is: beer wastewater 2.0L, water 10.0L, glucose 180g, urea 180.0g, K 2 HPO 4 9.0g; Inorganic salt: MgSO 4 5.5g, CaCO 3 5.0g, KCl 4.0g, etc.; trace elements: vitamin C 2.2g, ZnSO4 1.2g, FeCl 3 1.2g, adjust the pH value to 6.9-7.1 before sterilization.
[0029] All the other steps and conditions are the same as in Example 1-3.
Embodiment 7-9
[0031] The fermentation medium used is: MSG wastewater 2.0L, water 10.0L, sucrose 210.0g, starch 300.0g, K 2 HPO 4 9.6g; Inorganic salt: CaCO 3 6.0g, MgSO 4 5.0g, KCl 4.5g and other inorganic salts, vitamin C2.2g, ZnSO 4 1.5g, FeCl 3 1.5g, adjust the pH value to 6.9-7.0 before sterilization.
[0032] All the other steps and conditions are the same as in Example 1-3.
[0033] The seed medium and fermentation medium in the above examples were prepared according to the following proportions, and the same tests were carried out under the condition that the steps and other process conditions were unchanged, and the same effects were obtained.
[0034] Seed medium: (1) Biogas slurry 250ml, water 750ml, glucose 15g, K 2 HPO 4 0.5g, adjust the pH value to 7.0 before sterilization; (2) biogas slurry 170ml, water 830ml, glucose 20g, K 2 HPO 4 0.5g, adjust the pH value to 7.0 before sterilization; (3) Potato, sucrose or glucose (PD) liquid medium.
[0035] Fermentation m...
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