Method for preparing fermention medium, and producing biological pesticide by using wastewater from fermentation

A technology for fermenting medium and fermenting waste water, which is applied in the field of biopesticide production, and can solve problems such as failure to meet requirements, environmental pollution, and waste of resources, and achieve the effects of low pollution probability, good antibacterial effect, and low cost

Active Publication Date: 2007-02-21
CHINA NAT RICE RES INST
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no perfect treatment technology in the world. In our country, most of them are directly discharged into rivers, causing extremely serious environmental pollution and wasting precious resources.
Using yeast fermentation to treat monosodium glutamate wastewater to produce feed protein can turn waste into treasure, but the investment is large and the cost is high, and the treatment still does not meet the requirements
Abroad also use concentrated first isoelectric point extraction process to conduct evaporation and concentration treatment of monosodium glutamate wastewater. Because the evaporation and concentration process consumes a lot of energy, this method is not suitable for my country's national conditions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0023] The preparation of the seed medium used: biogas slurry 200ml, water 800ml, glucose 18g, K 2 HPO 4 0.4g, adjust the pH to 7.0 before sterilization.

[0024] The preparation of the fermentation medium used: biogas slurry 2.4L, water 9.6L, glucose (sucrose) 240g, urea 140g, K 2 HPO 4 9.5.0g, CaCO 3 5.0g, MgSO 4 4.5g, KCl 3.5g and other inorganic salts, vitamin C 2.0g, ZnSO 4 1.0g, FeCl 3 1.0g, adjust the pH value to 6.9-7.1 before sterilization.

[0025] Seed culture: Inoculate milky yellow bacteria such as Streptomyces griseus, Xanthomonas campestris pv.oryzae, Bacillus subtilis and other milky yellow bacteria into the seed medium respectively, and set the temperature at 100rpm and 28°C Shaking table shaking culture for 4 days, as a first-class seed fungus, similarly, the first-class seed fungus is expanded and cultivated according to the ratio of "first-class seed fungus: strain culture solution = 1: 10", as a second-class or large-scale seed fungus. Cultur...

Embodiment 4-6

[0028] The fermentation medium used is: beer wastewater 2.0L, water 10.0L, glucose 180g, urea 180.0g, K 2 HPO 4 9.0g; Inorganic salt: MgSO 4 5.5g, CaCO 3 5.0g, KCl 4.0g, etc.; trace elements: vitamin C 2.2g, ZnSO4 1.2g, FeCl 3 1.2g, adjust the pH value to 6.9-7.1 before sterilization.

[0029] All the other steps and conditions are the same as in Example 1-3.

Embodiment 7-9

[0031] The fermentation medium used is: MSG wastewater 2.0L, water 10.0L, sucrose 210.0g, starch 300.0g, K 2 HPO 4 9.6g; Inorganic salt: CaCO 3 6.0g, MgSO 4 5.0g, KCl 4.5g and other inorganic salts, vitamin C2.2g, ZnSO 4 1.5g, FeCl 3 1.5g, adjust the pH value to 6.9-7.0 before sterilization.

[0032] All the other steps and conditions are the same as in Example 1-3.

[0033] The seed medium and fermentation medium in the above examples were prepared according to the following proportions, and the same tests were carried out under the condition that the steps and other process conditions were unchanged, and the same effects were obtained.

[0034] Seed medium: (1) Biogas slurry 250ml, water 750ml, glucose 15g, K 2 HPO 4 0.5g, adjust the pH value to 7.0 before sterilization; (2) biogas slurry 170ml, water 830ml, glucose 20g, K 2 HPO 4 0.5g, adjust the pH value to 7.0 before sterilization; (3) Potato, sucrose or glucose (PD) liquid medium.

[0035] Fermentation m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention relates to a method for preparing fermentation culture medium and producing biological pesticide from fermentation wastewater. The method comprises: (1) utilizing fermentation liquid, ethanol wastewater and monosodium glutamate wastewater as the raw materials, and Streptomyces griseus as the strain; (2) fermenting at 28 deg.C and 100-150 rpm for 7-10 days; (3) filtering, concentrating and purifying to obtain biological pesticide. The method utilizes fermentation wastewater to prepare fermentation culture medium and produce biological pesticide, thus can effectively combine the treatment of fermentation wastewater and utilization of useful resources in fermentation wastewater. The treatment rate is high and the cost is low. Besides, the method for produing biological pesticide has such advantages as simple process, low cost, high productivity, and low probability of culture medium pollution and good antibacterial effect of the biological pesticide.

Description

technical field [0001] The invention belongs to the technical field of microbial wastewater treatment, in particular to a method for preparing a fermentation medium and producing biological pesticides by using fermentation wastewater. Background technique [0002] At present, most of industrial and agricultural fermentation wastewater in our country, such as biogas slurry, beer wastewater, and monosodium glutamate wastewater, have not been properly utilized, which not only wastes resources, but also pollutes the environment. For example, biogas slurry, due to its low nutrient content, is often used in a large amount when used as a fertilizer for crops, and the effect is poor. Therefore, farmers are reluctant to use biogas slurry as fertilizer and discharge it everywhere, which has become one of the important sources of rural environmental pollution. It is expensive and difficult to process. However, the biogas slurry still contains high nutrients...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C02F1/00
Inventor 黄世文王玲唐绍清朱德锋倪建平
Owner CHINA NAT RICE RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap