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ABO blood group inverse reagent

A technology of ABO blood type and anti-typing, applied in the direction of biological testing, material inspection products, etc., can solve the problems of blood type red blood cell complexion darkening and blackening, short storage period of blood type red blood cell, and high detection cost, so as to delay hemolysis and prevent antigen The effect of weakening and maintaining stability

Inactive Publication Date: 2007-03-07
BEIJING KINGHAWK PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The blood type anti-typing reagent prepared with Alfred's solution can be stored at 4°C for one month. Excluding the transportation and distribution time, it will only be valid for about 2 weeks when it reaches the end user, and the long-distance transportation will not take into account the stability of the cell membrane and cell viability. The impact of the detection cost is very high for many users
[0016] Another example is Japan's MAP liquid, AS-5 liquid, etc., which are not ideal because of the short storage period (only 1 month) and the complexion of red blood cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Preservation solution preparation:

[0144] Trisodium citrate 14.705g

[0145] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.408 g

[0146] Disodium hydrogen phosphate (Na 2 HPO 4 )0.716g

[0147] Disodium edetate 1.0 g

[0148] Sodium chloride (NaCl) 8.182 grams

[0149] Glucose monohydrate (Glucose) 19.810g

[0150] Adenine 0.405g

[0151] Inosine 2.682g

[0152] Chloramphenicol 0.66g

[0153] The above components were adjusted to 2 liters with distilled water.

[0154] preparation:

[0155] The above components were mixed and dissolved in distilled water to a constant volume, and 0.45 μl of a stainless steel high-pressure filter membrane was used to sterilize at 121° C. with 0.15 Mpa high-pressure steam for 30 minutes.

[0156] Blood type erythrocytes that have been accurately identified as blood type A are added to the above preservation solution for preparation, and the concentration of blood type erythrocytes is 3%.

[0157] Blood type erythrocytes w...

Embodiment 2

[0161] Preservation solution preparation:

[0162] Trisodium Citrate 10g

[0163] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.3 g

[0164] Disodium hydrogen phosphate (Na 2 HPO 4 ) 0.5 g

[0165] Disodium edetate 0.8 g

[0166] Sodium chloride (NaCl) 6 grams

[0167] Glucose monohydrate (Glucose) 15 grams

[0168] Adenine 0.3g

[0169] Inosine 2 grams

[0170] Chloramphenicol 0.5g

[0171] The above components were adjusted to 2 liters with distilled water.

[0172] preparation:

[0173] The above components were mixed and dissolved in distilled water to a constant volume, and 0.45 μl of a stainless steel high-pressure filter membrane was used to sterilize at 121° C. with 0.15 Mpa high-pressure steam for 30 minutes.

[0174] Blood type erythrocytes that have been accurately identified as blood type A are added to the above preservation solution for preparation, and the concentration of blood type erythrocytes is 3%.

[0175] Blood type erythrocytes with accuratel...

Embodiment 3

[0179] Preservation solution preparation:

[0180] Trisodium Citrate 20g

[0181] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.5 g

[0182] Disodium hydrogen phosphate (Na 2 HPO 4 ) 0.9 g

[0183] Disodium edetate 1.2g

[0184] Sodium chloride (NaCl) 10 grams

[0185] Glucose monohydrate (Glucose) 25 grams

[0186] Adenine 0.5g

[0187] Inosine 3 grams

[0188] Chloramphenicol 0.8g

[0189] The above components were adjusted to 2 liters with distilled water.

[0190] preparation:

[0191] The above components were mixed and dissolved in distilled water to a constant volume, and 0.45 μl of a stainless steel high-pressure filter membrane was used to sterilize at 121° C. with 0.15 Mpa high-pressure steam for 30 minutes.

[0192] Blood type erythrocytes that have been accurately identified as blood type A are added to the above preservation solution for preparation, and the concentration of blood type erythrocytes is 3%.

[0193] Blood type erythrocytes with accurat...

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PUM

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Abstract

This invention relates to one ABO blood reverse shape agent, which is based on the a's liquid and adopts the improved buffer system, that is from single citrate buffer system into citrate and phosphate double buffer system to better keep the system PH value stable, wherein, the invention adds gland purine as nutrient to make the erythrocyte keep bright color during abundant oxygen process; the anti-erosion system not adopting any chemical antigen to avoid impacting the oxygen content to make the red cell dark; adding EDTA sodium salt to prevent blood-stop cell hemolytic phenomenon.

Description

Technical field: [0001] The invention relates to a detection reagent, in particular to a blood detection reagent, in particular to a reverse typing blood detection reagent for ABO blood type. Background technique: [0002] Red blood cell blood type is the earliest human blood type discovered, and it is a genetic marker of human beings, which is of great significance to anthropology, genetics, blood transfusion, matching, organ transplantation and neonatal immune diseases. Since Landsteinor (1900) discovered the ABO blood type, Hoktoen proposed the importance of blood type identification for blood transfusion therapy as early as 1907, and Ottenberg et al. further proposed the concept of donor-recipient blood cross-mating experiment in 1908, emphasizing the importance of blood type identification for transfusion therapy. The far-reaching significance of clinical blood transfusion. Due to the importance of ABO blood type in blood transfusion, correct identification of ABO bloo...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/52
Inventor 张志曹健荣温重秋
Owner BEIJING KINGHAWK PHARMA
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