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Fungus culture separator and separation thereof

A strain separator and strain separation technology, which is applied in the direction of fungi, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of low success rate of large fungi and cumbersome inoculation procedures in the field, and achieve less mold pollution Effect

Inactive Publication Date: 2007-04-04
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings of the low success rate of separation and purification of large fungi in field work and the cumbersome inoculation process in the field, the present invention provides a fungal strain separator and a method for using it to separate fungal strains

Method used

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  • Fungus culture separator and separation thereof
  • Fungus culture separator and separation thereof
  • Fungus culture separator and separation thereof

Examples

Experimental program
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specific Embodiment approach 1

[0011] Specific embodiment one: as shown in Figure 1~3, the fungal strain separator of the present embodiment is filled with the upper part glass container 3 of sawdust medium 2 and the lower part glass container 4 that is filled with PDA medium 1 two parts The container is composed of an upper part glass container 3 connected with a lower part glass container 4 to prevent the internal and external air circulation from producing bacteria. The wall is punched with the aperture of diameter 2mm and the inside of the lower half pipe is equipped with sawdust medium 2, and the inside of lower part glass container 4 is filled with PDA medium 1, and sawdust medium 2 is communicated with PDA medium 1 by aperture.

[0012] The culture medium formula in the present embodiment is as follows:

[0013] ①PDA medium formula:

[0014] 200g peeled potatoes;

[0015] Glucose 20g;

[0016] Agar 20g;

[0017] 1000ml of water;

[0018] pH natural;

[0019] Sterilize at 121°C for 20 minutes. ...

specific Embodiment approach 2

[0026] Specific embodiment two: the present embodiment utilizes the fungal strain separator to separate the fungal strains, and the specific separation method is as follows:

[0027] Separation: Cut the large fungal tissues with an area of ​​about 0.5 cm into pieces and put them into the upper port of the upper part of the glass container 3. Other miscellaneous bacteria are not easy to use the improved sawdust medium 2 because the improved sawdust medium 2 does not contain sugar or contains The amount of sugar is extremely low, and the nutrient source of mold and other miscellaneous bacteria carbon is mainly simple sugars, so the growth here is limited, while large fungi can decompose and use cellulose and lignin as carbon sources, grow and spread normally, until Drill out in the aperture 5 of half section pipe wall, then utilize the nutrition in the lower part glass container 4 to continue to grow.

[0028] Purification: In the ultra-clean workbench, unplug the upper glass co...

specific Embodiment approach 3

[0030] Specific implementation mode three: This implementation mode is compared with previous separation and purification methods:

[0031] This embodiment is compared with previous separation and purification methods:

[0032] (1) In the past, the operation steps of isolation and purification of large fungi in the field or laboratory:

[0033] Materials: (1) sterilized test tube / plate PDA medium, (2) tweezers, (3) scalpel, (4) 75% alcohol, (5) 75% alcohol cotton, (6) sterile water, (7) ) 1% mercury liter, (8) alcohol lamp, (9) marker pen, (10) alcohol watering can, (11) matches, (12) sterilized empty culture dish.

[0034] Specific steps:

[0035] 1. Indoor sterilization - ordinary room in the field: spray alcohol into the room to precipitate indoor dust.

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Abstract

A fungus separator and its method for separating fungus are disclosed. The process is carried out by filling PDA culture medium(1) and chip culture medium(2) separately, connecting them together, decomposing chip culture(2) by fungus tissue sliced sheet, diffusing to PDA culture medium(1), selecting turf from PDA culture medium(1) onto slope of the ultra-clear operating table and culturing at 25degree. It's fast, convenient and correct, has higher separating purification ratio and no mold pollution.

Description

technical field [0001] The invention relates to a strain separator and a method for separating fungal strains by using the separator. Background technique [0002] In the field, the collected fresh fruiting bodies of large fungi are separated and purified. Due to the limitation of inoculation conditions, during the operation of obtaining pure strains, they are easily polluted by terrestrial and widely distributed molds, and the success rate is very low; Even if the fresh fruit bodies are transported back to the laboratory from the collection place, and the separation and purification are carried out in the ultra-clean workbench, the above-mentioned problems also exist; difficulty. Therefore, wild collection and on-site separation are advocated. This requires improvements in separation equipment. [0003] The distribution and types of macrofungi are closely related to the types of vegetation controlled by temperature and precipitation. The number of plant species in China ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/14
Inventor 赵敏孙立娜王炎
Owner NORTHEAST FORESTRY UNIVERSITY