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Mineralized polypeptide material in use for repairing bones, and preparation method

A bone repair and crystal technology, applied in the field of bioengineering, can solve problems such as hidden virus immune response, and achieve the effect of reducing hidden virus, excellent osteoinductive performance, and good biological activity.

Inactive Publication Date: 2007-05-09
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the currently used collagen is mainly derived from animals, there are risks of virus hidden dangers and immune reactions.

Method used

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  • Mineralized polypeptide material in use for repairing bones, and preparation method
  • Mineralized polypeptide material in use for repairing bones, and preparation method

Examples

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preparation example Construction

[0018] The method for preparing the above-mentioned mineralized polypeptide material for bone repair proposed by the present invention comprises the following steps:

[0019] (1) At a concentration of 1.5×10 -4 -1.5×10 -2 g / ml polypeptide acid solution or aqueous solution, add dropwise a solution containing calcium ions while stirring, the dropping amount is 0.003-0.053mol of calcium ions per gram of polypeptide, where the acid is one of hydrochloric acid, acetic acid, and nitric acid ;

[0020] (2) Slowly add dropwise the aqueous solution containing phosphate ion while stirring in the solution of step (1), the mol ratio of the phosphate ion added and calcium ion is Ca: P=1-2: 1;

[0021] (3) In the solution of step (2), add dropwise lye while stirring, and adjust the pH value to be 7.4-10;

[0022] (4) Stir the solution of step (3) and let it stand. After the precipitation and the supernatant are separated, remove the supernatant, centrifuge to separate the precipitation, ...

Embodiment 1

[0058] 1) Dissolve 60g of liquid polypeptide with a solid content of 1% in 300ml of deionized water, and add 40ml of CaCl with a concentration of 0.5mol / L dropwise 2 solution, continue to slowly add H at a concentration of 0.5 mol / L 3 PO 4 Solution 24ml, mix evenly with a magnetic stirrer while adding dropwise.

[0059] 2) Slowly add NaOH solution dropwise to the solution in the first step above while stirring until the pH value is stable at 7.4.

[0060] 3) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer for freeze drying, and then grind the dried product Get dry powder.

Embodiment 2

[0062] 1) Dissolve 70g of liquid polypeptide with a solid content of 1% in 4500ml of deionized water, and add 10ml of Ca(NO 3 ) 2 After the solution, continue to slowly drop Na with a concentration of 0.2mol / L 2 HPO 4 Solution 10ml, mix evenly with a magnetic stirrer while adding dropwise.

[0063] 2) Slowly add NaOH solution dropwise to the solution in the first step above while stirring until the pH value is stable at 10.0.

[0064] 3) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer for freeze drying, and then grind the dried product Get dry powder.

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Abstract

A mineralized polypeptide material used for treating bone defficiency and bone fracture features high bioactivity, biocompatibility and biodegradability. Its microstructure is a 3D network of polypeptide fibers with calcium-phosphorus salt crystals attached on their surfaces. The amino acid sequence of said po0lypeptide has the repeated Gly-X-Y structure, where X and Y may be any amino acid other than Gly.

Description

technical field [0001] The invention relates to a mineralized polypeptide material for bone repair and a preparation method thereof, belonging to the technical field of bioengineering. Background technique [0002] Currently, the autologous bone and allogeneic bone used in clinical bone repair have the risks of shortage, immune rejection and infection. The main components of biological bone tissue are collagen and hydroxyapatite. Pure hydroxyapatite has excellent biocompatibility, osteoconductivity and bioactivity, and has been widely used in plastic surgery and dental surgery. However, pure hydroxyapatite is brittle, has poor toughness, and is easy to fall off from the implant site, which limits its clinical use. In the prior art, the applicant once proposed a patent application titled "Preparation Method of Nanophase Calcium Phosphate Salt / Collagen / Polylactic Acid Bone Composite Porous Material" with application number 00107493.8 and publication number CN 1272383A. The ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/54A61L27/58
CPCA61K33/06A61K33/42A61K38/04A61K38/39A61L27/24A61L27/40
Inventor 崔福斋王玉胡堃
Owner TSINGHUA UNIV
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