Established cell line of microglia

a cell line and established technology, applied in the field of subcultivation established microglia, can solve the problems of difficult supplementary therapy, no other method than direct injection of the substance, and specific introduction into the brain, and achieve the effect of easy monitoring
US20040072346A1Inactive Publication Date: 2004-04-15JAPAN SCI & TECH CORP
0 Cites 2 Cited by

Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
JAPAN SCI & TECH CORP
Publication Date
2004-04-15
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

A subcultivatable, established microglia having the following properties. (a) Form: Both or either of a macrophage-like or spherical form in the presence of a granulocyte-macrophage colony stimulation factor and a branched form similar to branched microglia present in the brain in the absence of the factor. (b) Functional characteristics: specific affinity for the brain highly poor phagocytic action. (c) Cell proliferation: proliferative depending upon a granulocyte-macrophage colony stimulation factor. Preparation, separation, and screening methods of the microglia, a pharmaceutical composition using the microglia, and a method for treatment of cerebral diseases using the composition.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] The present invention relates to a subcultivatable, established cell line of microglia, a method of separating the same, and use thereof as a pharmaceutical carrier.

[0002] Further, the present invention relates to a microglia into which an extraneous gene or a drug was introduced, a method of introducing it, and a pharmaceutical composition comprising the same.

[0003] There are quite a number of hereditary diseases in the nervous system, which occur due to various causative factors, for example by defect of a single enzyme, etc. or by unknown reasons. Under these circumstances, supplementary therapy is used to cope with a large number of such diseases.

[0004] A large number of studies have been made worldwide on the system of selective delivery to the brain. For introducing a gene into the brain in an animal, a method of using neuronphilic viruses as adenovirus vectors is devised, and a system for introducing a gene specifically into neurons is known (Kozarsky, K. F and Wilson,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More