Methods for eradication of nanobacteria

a nanobacteria and nanotechnology, applied in the field of nanobacteria eradication, can solve the problems of nanobacteria with complicated nucleic acid repair systems, difficult detection with conventional microbiological methods, and very unlikely presence of nanobacteria in complex systems, so as to prevent the recurrence of kidney stones, inhibit or prevent the growth and development of nanobacteria, and prevent the infection of nanobacteria

Inactive Publication Date: 2005-02-17
KAJANDER OLAVI E +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

The present invention also provides methods of preventing nanobacterial infection, and treating patients infected with nanobacteria. In particular, the present invention provides a method for preventing the recurrence of kidney stones in a patient that has suffered from kidney stones, comprising administration of an antibiotic in an amount effective to inhibit or prevent the growth and development of nanobacteria.

Problems solved by technology

Their small size (0.05-0.5 μm), and unique properties make their detection difficult with conventional microbiological methods.
Nanobacteria possess unusual properties making their detection difficult with standard microbiological methods.
Because of their minimal size, the presence of complicated systems for nucleic acid repair in nanobacteria seems very unlikely.
The morbidity and expense that result from this disease is significant.
Apatite crystals also cause inflammation when injected into the synovial space.
Although pulp stones have been extensively studied morphologically, their origin is still obscure and little is known about their chemical composition.
Bone and tooth formation in the body have similar mechanisms, leaving many unanswered questions.
However, many details remain unresolved, including the specific nature of the initial precipitating phases, the mechanism and factors which control the incorporation of ionic impurities into the crystal lattice, details of the crystallographic ultrastructure and morphology in mineralized tissues (bone, dentine), and the relationship of the inorganic components with the complex collagen based matrix.
It has been shown that an accumulation of calcium in mitochondria, which is presumably dependent upon residual substrate for energy production, appeared to cause calcification.

Method used

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  • Methods for eradication of nanobacteria
  • Methods for eradication of nanobacteria
  • Methods for eradication of nanobacteria

Examples

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example 1

Mineralization by Nanobacteria

In this study, the present inventors provide evidence that nanobacteria can act as crystallization centers (nidi) for the formation of biogenic apatite structures. The mineralization process was studied in vitro with one bovine isolate from commercial fetal bovine serum and with a human isolate. These findings are of concern in medicine because nanobacterial bacteraemia occurs in humans, and nanobacterial nidi might initiate pathological calcification.

Materials and Methods

Culture Methods for Nanobacteria.

Nanobacteria were cultured in DMEM (GIBCO) under mammalian cell culture conditions (37° C.; 5-10% CO2 / 90-95% air). Serum was used at 10% final concentration as the supplement and source of nanobacteria, which were fetal bovine serum (Sera Lab, lot 901045), or human serum from a 29-years-old Finnish male. The cultures were prepared using strict aseptic techniques in a cell culture facility. Nanobacterial samples were filtered through 0.2 μm filt...

example 2

Eradication of Nanobacteria

The selection of an appropriate test for nanobacterial disinfection is not straightforward, and accurate comparisons of the results obtained from different tests are problematic, due to the number of factors affecting disinfection. These factors include duration of exposure, presence of organic load, type, age, concentration and diluent of the disinfectant, and number, age, growth form of the microorganisms present, and the temperature. Currently there are several types of disinfection tests, but these are mainly suitable for rapidly growing bacteria. The disinfection tests of slowly growing Mycobacteria, some of which are extremely resistant, have long suffered from lack of appropriate, reliable standardization. Typically, centrifugation or very high dilution have been used to eliminate the effect of residual concentrations of disinfectants. Subsequently, plating on agar medium for colony count is done for evaluating the reduction in viability. For nan...

example 3

Dental Pulp Stones Made by Nanobacteria

The purpose of the experiments conducted in this example was to investigate if nanobacteria participate in the dental pulp stone formation. The design of the study was to culture nanobacteria on a healthy tooth, without dental pulp stone, and compare the results with those obtained from a tooth having dental pulp stone. Mineral formations were observed under SEM. Additionally, an epidemiological screening was carried out on the possible correlation between dental pulp stone and kidney stone disease, and other bodily calcifications in 18 patients using a questionnaire.

Correlation between Dental Pulp Stones and Other Stone Formation in the Body.

18 patients were randomly selected from a private dental practice in Turkey based upon their periodontal problems caused by severe pulp stone formation. Collected pulp stones were stored in PBS containing 0.05% NaN3 at +4° C. The samples were demineralized in 1N HCl for 10 min at room temperature, n...

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Abstract

Nanobacteria contribute to pathological calcification in the human and animal body, including diseases such as kidney stones, salivary gland stones, dental pulp stones and atherosclerosis. The present invention provides methods for sterilizing articles contaminated with nanobacteria. The present invention also provides methods of treating patients infected with nanobacteria. In particular, the present invention provides a method for preventing the recurrence of kidney stones in a patient that has suffered from kidney stones, comprising administration of an antibiotic, a bisphosphonate, or a calcium chelator, either alone or in combination, in an amount effective to inhibit or prevent the growth and development of nanobacteria.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to methods of disinfecting articles infected with nanobacteria, and methods of treating patients infected with nanobacteria. 2. Description of the Related Art The formation of discrete and organized inorganic crystalline structures within macromolecular extracellular matrices is a widespread biological phenomenon generally referred to as biomineralization. Mammalian bone and dental enamel are examples of biomineralization involving apatite minerals. Environmental apatite stones have almost the same chemical composition as in bone and dentine. Recently, bacteria have been implicated as factors in biogeochemical cycles for mineral formation in aqueous sediments. The principal constituent of modern authigenic phosphate minerals in marine sediments is carbonate (hydroxy)fluorapatite Ca10(PO4)6−x(CO3)x(F,OH)2+x. Microorganisms are capable of depositing apatite outside thermodynamic equilibrium in sea ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N35/02A01N37/36A01N37/44A01N57/18A01N59/00A01N59/02A01N61/00A01N63/04A61K31/00A61K45/06A61L2/00A61L2/07A61L2/10A61L2/12A61L2/18
CPCA01N35/02Y10S424/06A01N37/44A01N57/18A01N59/00A01N59/02A01N61/00A01N63/04A61K31/00A61K45/06A61L2/0035A61L2/0088A61L2/07A61L2/081A61L2/10A61L2/12A61L2/186A61L2202/23A61L2202/24A01N37/36A01N41/04A01N37/42A01N33/12A01N2300/00
Inventor KAJANDER, OLAVI E.CIFTCIOGLU, NEVA
Owner KAJANDER OLAVI E
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