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Leucine-Rich Peptide Compositions and Methods for Isolation

a technology of leucine-rich peptides and compositions, applied in the field of leucinerich peptide compositions and methods for their isolation from proteins, can solve the problems of difficult digestion and/or absorption of proteins, and achieve the effects of increasing nitric oxide production, decreasing blood pressure, and increasing blood flow

Inactive Publication Date: 2009-10-22
GLANBIA NUTRITIONALS IRELAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]The present invention relates to peptides having a leucine content of from about 12% to about 40% (“leucine-rich peptides”), those peptides being isolated from a protein source by a method comprising the steps of hydrolyzing the protein in the presence of one or more leucyl aminopeptidases, optionally in combination with additional proteases, to produce a protein hydrolysate, deactivating the enzymes, and filtering the protein hydrolysate to provide a permeate comprising peptides having a molecular weight of from about 200 to about 4,000, including sub-ranges in-between, such as, for example, a molecular weight of from about 200 to about 1,000 or a molecular weight of from about 400 to about 1,000. Optionally, the step of concentrating the leucine-rich peptides by removing the aqueous fluid from the permeate may be added. Also disclosed are method of use of such peptides for a variety of beneficial effects, such as producing vasodilation, increasing nitric oxide production, decreasing blood pressure, increasing blood flow, increasing muscle tissue, improving wound healing, and improving cognitive function.

Problems solved by technology

Whey proteins are a good source of leucine, but for many individuals who need the muscle-building amino acids dietary proteins can provide, it is difficult to digest and / or absorb proteins.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Production of Leucine Peptides

[0030]A high protein, low fat, low lactose liquid whey protein isolate product was used as the beginning substrate. This product was pumped into reaction tanks at a solids level of 17%. The temperature was raised to 45° C. and pH adjusted to 7.3 with sodium hydroxide. A blend of proteases and aminopeptidases (e.g., leucine aminopeptidase) was then added at a level of 0.35% of the solids. Hydrolysis was allowed to proceed for a 6-hour period with hourly pH adjustments to maintain the pH at 7.3. The enzymes were then deactivated by heating the solution to 65° C.

[0031]Fractionation was performed using filtration, the hydrolysate being passed through the filter to retain molecules having a molecular weight of greater than 20,000. The permeate coming from filtration system contained the leucine peptides, having approximately 52% of the peptides in the molecular weight range of less than 1,000; 41% of the peptides in the molecular weight range of 1,000 to 4,0...

example 2

Stimulation of Increased Blood Flow, Vasodilation, NO Production

[0033]Individuals were provided with 2 weeks of daily supplementation with either the peptide product of the invention, or placebo. After completion of the first 2 week supplementation period and first day of vascular testing there was an interval of 1-2 weeks, after which the individuals started the second 2 week supplementation period, each individual consuming the alternative supplement. Individuals were evaluated at four separate times during the protocol, and asked to fast for 12 hours, as well as to avoid alcohol, caffeine, and exercise for 24 hours, and to consume 36 ounces of water the night before each evaluation and an additional 12 to 16 ounces of water the morning of the visit, to ensure that each person was adequately hydrated.

[0034]The peptide product was produced by Glanbia Nutritionals, Twin Falls, Id. A single dose of 5 g was premeasured and placed in individual packets with artificial sweetener. Indivi...

example 3

ACE Inhibition Assay

[0041]The ACE inhibition assay has been previously described by Cushman and Cheung (Cushman, D. W. and Cheung, H. S., Biochem. Pharmacol. (1971) 20: 1637). Briefly, substrate was prepared by dissolving 21.475 mg of Hip-His-Leu (“HHL,” Sigma, St. Louis, Mo.) in 8 ml of phosphate buffered saline, with volume adjusted to 10 ml and final pH to 8.3. A 10% w / w solution of peptide composition was prepared using buffer as diluent. Angiotensin-converting enzyme stock solution was prepared by diluting 0.1 unit of ACE (rabbit lung, Sigma Chemical, St. Louis, Mo.) with buffer. To perform the assay, 10 microliters of sample were placed into a small glass tube with 200 μl of HHL and 70 μl of buffer. Tubes were placed in a 37° C. water bath and held for 3 minutes. Tubes were removed from the water bath and 20 μl of angiotensin-converting enzyme were added to each tube. Tubes were vortexed and returned to the water bath for 30 minutes. Tubes were then removed from the water bath...

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Abstract

Disclosed are compositions comprising isolated peptides having a leucine content of from about 12 to about 40 weight percent. Also disclosed is a method for isolating leucine-rich peptides from protein sources such as bovine whey and methods of use for these peptides to provide beneficial effects in a human and / or animal such as increasing blood flow, decreasing blood pressure, increasing muscle mass, improving cognitive function, improving cardiovascular function, etc.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of priority of earlier-filed U.S. provisional patent application No. 61 / 039,426, filed Mar. 26, 2008.FIELD OF THE INVENTION[0002]The invention relates to dietary peptide compositions for stimulating protein synthesis, decreasing protein degradation, producing vasodilation, increasing nitric oxide production, and decreasing blood flow. More specifically, the invention relates to leucine-rich peptide compositions and methods for their isolation from proteins.BACKGROUND OF THE INVENTION[0003]Loss of muscle tissue often occurs as a result of aging, malnutrition, and catabolic diseases such as burns, sepsis, and cancer. Dietary protein supplementation may be beneficial, but supplementation with the essential amino acid leucine has been shown to be especially beneficial. Dietary leucine has, for example, recently been shown to suppress the rate of myofibrillar protein degradation and muscle weight loss in rat...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/16C07K1/34A61P9/10
CPCA23L1/3051C12P21/06A61K38/018A23L1/3053A61K31/198A23L33/175A23L33/18A61P3/02A61P7/00A61P9/00A61P9/08A61P9/10A61P9/12A61P43/00A23V2002/00
Inventor WARD, LOREN S.PETERSON, BRENT L.WROBEL, STANLEYBASTIAN, ERIC D.
Owner GLANBIA NUTRITIONALS IRELAND
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