Hemicellulase supplement to improve the energy efficiency of hemicellulose-containing animal feed

a technology of hemicellulose and hemicellulose, which is applied in the direction of fruit/vegetable preservation using acids, food preparation, enzymology, etc., can solve the problem of costly storage facilities of feed components, and achieve the effect of enhancing energy content when consumed

Inactive Publication Date: 2003-03-25
ELI LILLY & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

It is therefore an object of the present invention to provide a hemicellulase-containing composition that, because it also contains a novel hemicellulase, has an enhanced energy content when consumed.

Problems solved by technology

As a result, costly storage facilities for the feed components are necessary for the blending operations.
These carbohydrates are not absorbed to any appreciable degree by monogastric animals as the animals are unable to digest them rapidly enough to obtain the appropriate monosaccharide for further biochemical oxidation.

Method used

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  • Hemicellulase supplement to improve the energy efficiency of hemicellulose-containing animal feed
  • Hemicellulase supplement to improve the energy efficiency of hemicellulose-containing animal feed
  • Hemicellulase supplement to improve the energy efficiency of hemicellulose-containing animal feed

Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation of Hemicellulase-Producing Microorganisms from Soil

Soil samples collected from both a tropical rain-forest and a temperate garden were added to selective enrichment broth in a 10% w / v concentration. The cultures were shaken in baffled Erlenmeyer shake flasks at 37.degree. C. for four days. Four dilutions [1:10, 1:20, 1:800 and 1:50,000] of the initial cultures were made with fresh selective enrichment broth and incubated at 37.degree. C. for four days. The 1:50,000 dilution was used to prepare a dilution series (10.sup.-1 to 10.sup.-8) in 0.85% NaCl which were plated out on selective enrichment agar and incubated at 34.degree. C. for 5-7 days. Following incubation, isolated colonies were selected from the plated cultures and streaked for purity three successive times on a suitable agar medium. Nine isolates from soil collected from a tropical rain-forest and 24 isolates from soil collected from a temperate garden were selected as a result of this screening process.

example 2

Screening of Soil Isolates for Hemicellulase Activity

Each purified isolate from the tropical rain-forest soil was transferred to a shake flask containing selective enrichment broth and shaken for 48-72 hours at 34.degree. C. After incubation, the culture was centrifuged for 20-30 minutes at 10,000 rpm (4.degree. C.). The resultant supernatant was filtered sequentially through a 0.8- and a 0.45-micron filter to recover crude enzyme free of microbial cells. Each purified isolate from the temperate garden soil was transferred to tubes containing selective enrichment broth and shaken 12-48 hours at 34.degree. C. After incubation, the cultures were centrifuged and filtered as described above. The crude enzyme solution was added to tubes containing 5 mL of a cross-linked guar preparation (5.0 g guar, 2.0 g (NH.sub.4).sub.2 SO.sub.4 and 0.6 g sodium tetraborate per 400 mL water, pH 9.5) and incubated for at least 1 hour in a water bath at 39.degree.-40.degree. C. To measure enzyme activity...

example 3

Production of Commercially Useful Quantities of Hemicellulase

A single, isolated colony of Bacillus circulans (CMG1240) was inoculated into a 500 mL Erlenmeyer baffled shake (pre-seed) flask containing 50 mL of brain heart infusion broth. The inoculated flask was shaken (300 rpm) while incubating for 12-16 hours at 35.degree. C. After incubation, the entire volume of the pre-seed flask was aseptically transferred to a 4 L Erlenmeyer baffled shake-flask (seed) containing one liter of seed medium. The seed-flask was incubated with shaking for 12-16 hours at 35.degree. C. The entire volume of the seed flask was aseptically transferred to a 14-liter fermenter containing 9.0 liters of fermentation broth. The fermenter was maintained at 40.degree. C., <20% dissolved oxygen and 500-1000 rpm. Guar gum was added to make a final concentration of 0.05% to the fermenter two or three times during the growth phase and once (0.5%) during the stationary phase to induce enzyme production. After 1-7.5...

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Abstract

Soil microorganisms are obtained that produce a hemicellulase which is particularly useful in increasing the available energy content of hemicellulosic foodstuffs. These microorganisms can be cultured per se or can be used as sources of genetic information with which to engineer other microorganisms to produce the enzyme. Thus, commercially useful quantities of native or recombinant hemicellulase can be produced with cultures consisting essentially of microorganisms that produce the enzyme. The hemicellulase can then be employed in a feed composition containing complex carbohydrates which the enzyme degrades, enhancing the nutritional value of the composition.

Description

BACKGROUND OF THE INVENTIONDifferent enzymes are categorized as a specific type of hemicellulase--a glucanase, a xylanase or a mannanase, for example--based on an ability to catalyze the hydrolysis of heteropolysaccharides composed of glucan, xylan or mannan, respectively. It is known that enzymes that effect hydrolysis of mannans, such as a galactan or a glucomannan, are produced by various microorganisms, including bacteria and fungi, and that they also occur in some animals and in numerous plants. Among the microorganisms that produce such mannanases are species of Aeromonas, Aspergillus, Streptomyces, Rhodococcus and Bacillus. See 160 METHODS IN ENZYMOLOGY Part A, Sect. II (1988).Hemicellulases have been employed commercially in the processing of coffee, chocolate, cocoa, tea and cereals. The primary advantage gained by using a hemicellulase in this regard is a reduction in solution viscosity which allows for more inexpensive processing of food products. Thus, hemicellulases are...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A23K1/00A23K1/165A23L1/03A23L1/052A23L1/0534C12N9/24A23K1/16A23K1/175A23L11/00A23L29/00A23L29/262C12N9/42C12R1/07
CPCC12Y302/01078C12Y302/01101C12N9/2437C12N9/2494A23K10/10A23K20/189A23L29/06A23L29/262
Inventor FODGE, DOUGLAS W.ANDERSON, DAVID M.
Owner ELI LILLY & CO
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